Naglaa Fathy Abbas

Publications (2)1.5 Total impact

• Article: Clinical utility of Glycan-3 in Hepatocellular carcinoma

  Mona Fathy Youssef, Sonia Labib El-Sharkawy, Naglaa Fathy Abbas, El-Shinnawy Samah, El-Hadidi Eman, Shahin Rania, Shalabi Hend
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  ABSTRACT: Early diagnosis of hepatocellular carcinoma (HCC) is critical for a good prognosis. This study aimed to evaluate the clinical utility of serum and tissue glypican-3 (GPC3) as well as its messenger RNA (mRNA) as diagnostic and prognostic marker in HCC in comparison to alpha fetoprotein (AFP). Forty HCC patients, 20 cirrhotic patients and 20 healthy controls were subjected to measurement of serum AFP (sAFP) and serum GPC3 (sGPC3) by enzyme immunoassay, GPC3mRNA assay in peripheral blood samples using reverse transcription polymerase chain reaction (RT-PCR), in addition to immunohistochemical analysis of GPC3 in liver resection specimens. GPC3 was detected immunohistochemically in 97.5% of HCC cases. The positive rate of GPC3 mRNA in blood samples was 95% in HCC patients. The positive rate of GPC3 mRNA in blood samples was 100% in HCC patients. Detection of GPC3 (by immunohistochemistry and PCR) was associated with clinical markers of HCC. Each of sGPC3 and sAFP showed a statistical significant difference between HCC patients and controls. It is concluded that GPC3 is a promising diagnostic and prognostic marker of HCC. However simultaneous assay of sGPC3 and sAFP is important for diagnosis of HCC and to follow-up high risk patients especially those with detectable levels of sGPC3.
  International Journal of Integrative Biology. 01/2010;
• Article: Immunohistochemical study of p53 and angiogenesis in benign and preneoplastic oral lesions and oral squamous cell carcinoma.

  Naglaa Fathy Abbas, Sonia Labib El-Sharkawy, Effat Ahmed Abbas, Marwa Abdel Monem El-Shaer
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  ABSTRACT: Mutation of the p53 gene and overexpression of its protein are frequent in human cancer, suggesting that these events play a critical role in the onset or progression of many types of tumors. Angiogenesis also has been demonstrated to be associated with tumor progression, aggressiveness, and metastases. This study aimed to investigate-in normal oral epithelium, hyperplasia, dysplasia, and invasive oral squamous cell carcinoma-the prevalence of p53 protein immunoreactivity and of angiogenesis, and to determine the correlation between them. The study was performed on tissue sections of hyperplasia (n = 14), dysplasia (n = 10), and invasive squamous cell carcinoma (n = 21). Seven normal samples of oral mucosa were used as control. Angiogenesis and p53 protein expression were investigated by means of immunohistochemistry. This study showed that p53 protein was confined to the basal cell layer in normal oral mucosa and in the hyperplastic group. In the dysplastic group, it was expressed in the basal and suprabasal layer, whereas in invasive carcinoma, it was detected in central and peripheral regions. The percentage of p53-positive cells was evaluated, and statistically significant differences were found between normal oral mucosa and severe dysplasia, between normal mucosa and invasive carcinoma, and between mild and severe dysplasia. p53 expression showed no significant correlation with tumor grading. Angiogenesis was assessed using the endothelial cell marker von Willebrand's factor. The number and size of blood vessels increased from normal oral epithelium through dysplastic epithelium to reach a maximum in invasive carcinoma. Angiogenesis and p53 protein immunoreactivity increased with progression from normal mucosa to invasive carcinoma, and both factors were directly correlated to each other suggesting that they may play a critical role in oral carcinogenesis.
  Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics 04/2007; 103(3):385-90. · 1.50 Impact Factor