Münir Aktaş

Firat University, Mezreh, Elazığ, Turkey

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Publications (12)5.58 Total impact

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    ABSTRACT: This study was carried out to determine the presence and frequency of Anaplasma ovis and Anaplasma phagocytophilum in small ruminants from Bingol, Elazig, Malatya and Mus provinces. A total of 422 (291 sheep and 131 goats) blood samples were collected from apparently healthy animals. To determine of A. ovis and A. phagocytophilum in small ruminants, species-specific PCRs were set up using 60 kDA chaperonin gene (cpn60, also known as hsp60 or groEL) and 16S SSU rRNA gene primer sets, respectively. A total of 301 (71.32%) animals were found infected with A. ovis and/or A. phagocytophilum. The percentages of positive animals for A. ovis and A. phagocytophilum were 67.06% (283/422) and 19.66% (83/422), respectively. The rate of concurrent infections was 15.40% (65/422). Four PCR products from positive samples were purified from agarose gel and sequenced. These sequences were identical to the reported nucleotide sequences of A. ovis and A. phagocytophilum. This is the first molecular based study on the detection of A. phagocytophylum and A. ovis in small ruminants from East Anatolia Region. Further studies are needed on the determination of the genotypes and vectors of the species. Doğu Anadolu Bölgesinde Koyun ve Keçilerde Anaplasma Enfeksiyonlarının Araştırılması Özet Bu çalışma Bingöl, Elazığ, Malatya ve Muş yöresindeki koyun ve keçilerde Anaplasma ovis ve Anaplasma phagocytophilum'un araştırılması amacıyla yapılmıştır. Rastgele seçilen 422 sağlıklı (291 koyun 131 keçi) hayvandan kan örneği alınmıştır. A. ovis için 60 kDA chaperonin gen (cpn60, hsp60 veya gro EL olarak da bilinir) ve Anaplasma phagocytophilum için 16S SSU rRNA genlerine spesifik primerler kullanılarak tür spesifik PCR yapılmıştır. PCR sonucunda toplam 301 (%71.32) hayvan A. ovis ve/veya A. phagocytophilum ile enfekte bulunmuştur. Örneklerin %67.06 (283/422)'sı A. ovis, %19.66 (83/422)'sı A. phagocytophilum ve %15.40 (65/422)'ı her iki tür yönünden pozitif olarak tespit edilmiştir. A. ovis ve A. phagocytophilum yönünden pozitif olan PCR ürünleri agaroz jelden purifiye edilerek sekanslanmıştır. Elde edilen DNA dizilimlerin daha önce bildirilen A. ovis ve A. phagocytophilum'a ait dizilimlerle aynı olduğu görülmüştür. Bu çalışma Doğu Anadolu Bölgesinde koyun ve keçilerde A. ovis ve A. phagocytophilum'un moleküler yöntemlerle teşhisi üzerine yapılan ilk araştırmadır. Türlerin genotipleri ve vektörlerinin belirlenmesine yönelik çalışmaların gerektiği düşünülmektedir. Makale Kodu (Article Code): KVFD-2013-9189 Anaplasma species are known to be important tick-borne pathogens of humans and animals. The genus Anaplasma comprises A. phagocytophilum (previously recognised as Ehrlichia equi and E. phagocytophila), A. centrale, A. marginale, A. bovis, A. ovis and A. platys. The species are mainly transmitted by ixodid ticks [1-4] .
    Kafkas Üniversitesi Veteriner Fakültesi Dergisi 01/2014; · 0.46 Impact Factor
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    ABSTRACT: This study was carried out to determine the presence and frequency of Anaplasma ovis and Anaplasma phagocytophilum in small ruminants from Bingol, Elazig, Malatya and Mus provinces. A total of 422 (291 sheep and 131 goats) blood samples were collected from apparently healthy animals. To determine of A. ovis and A. phagocytophilum in small ruminants, species-specific PCRs were set up using 60 kDA chaperonin gene (cpn60, also known as hsp60 or groEL) and 16S SSU rRNA gene primer sets, respectively. A total of 301 (71.32%) animals were found infected with A. ovis and/or A. phagocytophilum. The percentages of positive animals for A. ovis and A. phagocytophilum were 67.06% (283/422) and 19.66% (83/422), respectively. The rate of concurrent infections was 15.40% (65/422). Four PCR products from positive samples were purified from agarose gel and sequenced. These sequences were identical to the reported nucleotide sequences of A. ovis and A. phagocytophilum. This is the first molecular based study on the detection of A. phagocytophylum and A. ovis in small ruminants from East Anatolia Region. Further studies are needed on the determination of the genotypes and vectors of the species. Doğu Anadolu Bölgesinde Koyun ve Keçilerde Anaplasma Enfeksiyonlarının Araştırılması Özet Bu çalışma Bingöl, Elazığ, Malatya ve Muş yöresindeki koyun ve keçilerde Anaplasma ovis ve Anaplasma phagocytophilum'un araştırılması amacıyla yapılmıştır. Rastgele seçilen 422 sağlıklı (291 koyun 131 keçi) hayvandan kan örneği alınmıştır. A. ovis için 60 kDA chaperonin gen (cpn60, hsp60 veya gro EL olarak da bilinir) ve Anaplasma phagocytophilum için 16S SSU rRNA genlerine spesifik primerler kullanılarak tür spesifik PCR yapılmıştır. PCR sonucunda toplam 301 (%71.32) hayvan A. ovis ve/veya A. phagocytophilum ile enfekte bulunmuştur. Örneklerin %67.06 (283/422)'sı A. ovis, %19.66 (83/422)'sı A. phagocytophilum ve %15.40 (65/422)'ı her iki tür yönünden pozitif olarak tespit edilmiştir. A. ovis ve A. phagocytophilum yönünden pozitif olan PCR ürünleri agaroz jelden purifiye edilerek sekanslanmıştır. Elde edilen DNA dizilimlerin daha önce bildirilen A. ovis ve A. phagocytophilum'a ait dizilimlerle aynı olduğu görülmüştür. Bu çalışma Doğu Anadolu Bölgesinde koyun ve keçilerde A. ovis ve A. phagocytophilum'un moleküler yöntemlerle teşhisi üzerine yapılan ilk araştırmadır. Türlerin genotipleri ve vektörlerinin belirlenmesine yönelik çalışmaların gerektiği düşünülmektedir. Makale Kodu (Article Code): KVFD-2013-9189 Anaplasma species are known to be important tick-borne pathogens of humans and animals. The genus Anaplasma comprises A. phagocytophilum (previously recognised as Ehrlichia equi and E. phagocytophila), A. centrale, A. marginale, A. bovis, A. ovis and A. platys. The species are mainly transmitted by ixodid ticks [1-4] .
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    ABSTRACT: Crimean-Congo hemorrhagic fever virus (CCHFV) is the causative agent of a tick-borne disease with high mortality rates in humans. The distribution of CCHFV includes over 30 countries in Asia, the Middle East, southeastern Europe, and Africa. It was first recognized in Turkey in 2002, with an increasing number of cases reported between 2002 and 2009. Recent analysis of complete genome sequences of CCHFV isolates has revealed that the genomic plasticity of the virus is surprisingly high for an arthropod-borne virus. We have determined the complete nucleotide and deduced amino acid sequences of strain CCHFV Turkey-Kelkit06 isolated from the blood of a patient in an endemic region of Turkey in 2006. The complete sequence length of the CCHFV Turkey-Kelkit06 strain is 19,186 nt, consisting of a 1673 nt S segment, a 5364 nt M segment, and a 12,149 nt L segment. Based on the analysis of S, M, and L segments, CCHFV Turkey-Kelkit06 clustered in Group V, which represents the Europe/Turkey geographic lineage. Although glycoproteins encoded by the M gene are the most variable part of the CCHFV Turkey-Kelkit06 strain, some functional domains of the glycoproteins are well conserved. Here, we report the complete sequence and genome organization of the CCHFV Turkey-Kelkit06 strain and its phylogenetic relationship to other strains of CCHFV. Collecting data on viral sequences among isolates from CCHF epidemics may provide valuable information regarding the molecular basis of the epidemic potential of the virus.
    Virus Research 11/2009; 147(2):288-93. · 2.75 Impact Factor
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    ABSTRACT: The species causing theileriosis in cattle in Turkey are Theileria annulata and T. buffeli. While T. buffeli is low in pathogenicity or non-pathogenic , T. annulata is very pathogenic and causes tropical theileriosis with high morbidity and mortality in cattle. In this study, a multiplex PCR was used for a simultaneous diagnosis of these species. Genes for the merozoite surface antigen (Tams 1) and the major piroplasm surface protein (MPSP) were amplified with PCR for T. annulata and T. buffeli, respectively. It was found that both single and mixed infection with T. annulata and T. buffeli could be diagnosed with multiplex PCR.
    Turkiye parazitolojii dergisi / Turkiye Parazitoloji Dernegi = Acta parasitologica Turcica / Turkish Society for Parasitology 02/2008; 32(1):1-3.
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    ABSTRACT: The genomic region spanning the two ribosomal RNA internal transcribed spacers (ITS1 and ITS2) and the 5.8S rRNA gene was cloned and sequenced from sixteen Theileria isolates. Each Theileria species possessed ITS1 and ITS2 of unique size(s) and species specific nucleotide sequences. Varying degrees of ITS1 and ITS2 intra- and inter-species sequence polymorphism were found among ruminant Theileria species. The spacers were most polymorphic in the agent of tropical theileriosis, Theileria annulata, and were more conserved in two benign species, Theileria buffeli and Theileria sergenti Chitose. Phylogenetic analysis of the rDNA ITS1-5.8S rRNA gene-ITS2 region clearly separated each taxon, placing them in three clusters. One held T. annulata, Theileria parva, and Theileria mutans, with the latter two most closely related. The second held T. sergenti Ikeda, T. sergenti Chitose, and T. buffeli, with the latter two most closely related. The third cluster held the Theileria ovis isolates.
    Veterinary Parasitology 08/2007; 147(3-4):221-30. · 2.38 Impact Factor
  • Kürşat Altay, Münir Aktaş, Nazir Dumanli
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    ABSTRACT: This study was carried out to investigate Theileria annulata and T. buffeli/orientalis in cattle in the region of Erzincan using reverse line blotting (RLB) and microscopical examination. A total of 123 blood samples and thin blood smears were collected from cattle in distinct locations. Thin blood smears were microscopically examined for Theileria piroplasms. The 18S SSU rRNA gene in the DNA of Theileria spp extracted from blood was amplified and used in RLB. For this purpose, PCR products were hybridized with specific probes for over-all Theileria spp., T. annulata and T. buffeli/orientalis as well as Babesia spp. While Theileria spp. were observed in 14 out of 123 cattle, (11.38 %) during microscopical examination, T. annulata was detected in 19 (15.45%) cattle and T. buffeli/orientalis, in 12 (9.76%) by RLB, respectively. Mixed infection was also detected in three samples.
    Turkiye parazitolojii dergisi / Turkiye Parazitoloji Dernegi = Acta parasitologica Turcica / Turkish Society for Parasitology 02/2007; 31(2):94-7.
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    Kürşat Altay, Münir Aktaş, Nazir Dumanli
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    ABSTRACT: This study was carried out to determine the prevalence of Theileria (T.) ovis and to investigate the presence of T. lestoquardi in small ruminants by microscopic examination (ME) and polymerase chain reaction (PCR) in the East and Southeast Anatolia. Whole blood samples (677 sheep and 142 goats) and thin blood smears (656 sheep and 139 goats) were collected from Malatya, Muş, Erzincan, Erzurum, Iğdir, Diyarbakir and Mardin. Piroplasms of Theileria spp. were detected in 18.29% (120/656) of sheep and 2.88% (4/139) of goats by ME. T. ovis was detected in 58.79% (398/677) of sheep and 11.27% (16/142) of goats by PCR whereas T. lestoquardi was not detected in the same animals.
    Turkiye parazitolojii dergisi / Turkiye Parazitoloji Dernegi = Acta parasitologica Turcica / Turkish Society for Parasitology 02/2007; 31(4):268-71.
  • Kürşat Altay, Münir Aktaş, Nazir Dumanli
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    ABSTRACT: Tams1 is a merozoite surface antigen of Theileria annulata. Genetical variations of Tams1 make studies of vaccine and diagnostic tests like ELISA difficult. In this study, Tams1 genes of 89 T. annulata isolates obtained from natural infected cattle in Elazig and Bingöl regions were tested with PCR-RFLP. Six different restriction profiles (a, b, c, d, e, f) were detected. The number of restriction profiles of 89 samples was found to be as follows: 78(a), 2(b), 2(c), 5(d), 1(e), and 1(f).
    Turkiye parazitolojii dergisi / Turkiye Parazitoloji Dernegi = Acta parasitologica Turcica / Turkish Society for Parasitology 02/2007; 31(3):173-5.
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    ABSTRACT: ZET: Tams1, Theileria annulata'nın merozoit yüzey antijeni olup, bu antijendeki genetik farklılıklar ELISA gibi tanı amaçlı test gel-iştirme ve rekombinant aşı çalışmalarını zorlaştırmaktadır. Bu çalışmada, Elazığ ve Bingöl ilerinde, doğal enfekte sığırlardan elde edilen 89 T. annulata izolatının, Tams1 geninin PCR-RFLP analizi yapıldı. PCR-RFLP sonucunda, 6 farklı restriksiyon profili (a, b, c, d, e, f) tespit edildi. 89 örneğin restriksiyon profillerinin dağılımının 78(a), 2(b), 2(c), 5(d), 1(e), 1(f) olduğu belirlendi. SUMMARY: Tams1 is a merozoite surface antigen of Theileria annulata. Genetical variations of Tams1 make studies of vaccine and diagnostic tests like ELİSA difficult. In this study, Tams1 genes of 89 T. annulata isolates obtained from natural infected cattle in Elazig and Bingöl regions were tested with PCR-RFLP. Six different restriction profiles (a, b, c, d, e, f) were detected. The number of restriction profiles of 89 samples was found to be as follows: 78(a), 2(b), 2(c), 5(d), 1(e), and 1(f).
    Türkiye Parazitol Derg. 01/2007; 31:173-175.
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    ABSTRACT: : This study was carried out to determine the prevalence of Theileria (T.) ovis and to investigate the presence of T. lestoquardi in small ruminants by microscopic examination (ME) and polymerase chain reaction (PCR) in the East and Southeast Anatolia. Whole blood samples (677 sheep and 142 goats) and thin blood smears (656 sheep and 139 goats) were collected from Malatya, Muş, Erzincan, Erzurum, Iğdır, Diyarbakır and Mardin. Piroplasms of Theileria spp. were detected in 18.29% (120/656) of sheep and 2.88% (4/139) of goats by ME. T. ovis was detected in 58.79% (398/677) of sheep and 11.27% (16/142) of goats by PCR whereas T. lestoquardi was not detected in the same animals. Doğu ve Güneydoğu Anadolu Bölgelerinde Küçük Ruminantlarda Theileria Enfeksiyonları ÖZET: Bu çalışma, mikroskopik bakı ve polimeraz zincir reaksiyonu (PCR) ile Doğu ve Güneydoğu Anadolu bölgelerinde koyun ve keçilerde Theileria ovis'in yaygınlığının belirlenmesi ve T. lestoquardi'nin varlığının araştırılması amacıyla yapıldı. Malatya, Muş, Erz-incan, Erzurum, Iğdır, Diyarbakır ve Mardin illerindeki koyun ve keçilerden kan örnekleri (677 koyun ve 142 keçi) ve kan frotileri (656 koyun ve 139 keçi) alındı. Kan frotilerinin mikroskopik muaynesinde koyunların %18,29 (120/656)'unda, keçilerin %2,88 (4/139)'unda Theileria spp. piroplasmları belirlendi. PCR ile koyunların %58,79 (398/677)'sinde, keçilerin %11,27 (16/142)'sinde T. ovis tespit edilirken, T. lestoquardi bulunamadı.
    Türkiye Parazitol Derg. 01/2007; 31:268-271.
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    ABSTRACT: This study was carried out in order to investigate the presence of Neospora caninum in cattle in the Elaziğ, Malatya, Muş and Bingöl provinces from January 2003- May 2004. Blood samples were collected from 513 cattle and 32 aborting cows that were of various breeds and ages in all provinces. Sera were obtained from these animals and antibodies against N. caninum were investigated using the enzyme-linked immunosorbent assay (ELISA). A commercially available competitive ELISA (cELISA) kit (VMRD, Inc., Veterinary Medical Research and Development, Pullman, WA, USA. Product Code 5NO5.20) was used to detect the N. caninum antibodies in the sera. Out of 513 cattle in the region, 36 (7.01 %) were found to be seropositive by cELISA. Seropositivity rates obtained by cELISA were 15.00% in Elaziğ, 4.00% in Malatya, 4.86% in Muş and 4.69% in Bingöl. One of the 32 aborting cows (3.12 %) was found to be seropositive against N. caninum.
    Turkiye parazitolojii dergisi / Turkiye Parazitoloji Dernegi = Acta parasitologica Turcica / Turkish Society for Parasitology 02/2005; 29(1):22-5.
  • Münir Aktaş, Nazir Dumanli, Kürşat Altay
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    ABSTRACT: This study was carried out in order to investigate the presence of Theileria ovis in small ruminants in the Elazig region between April-October 2004. A total of 164 whole blood and thin blood smears (from 103 sheep and 61 goats) were collected from 15 flocks in different locations. T. ovis piroplasm DNA extracted from sheep and goats' blood was amplified by the polymerase chain reaction (PCR) using specific primers. Thin blood smears were examined for Theileria piroplasms by microscopic examination. In the examination of DNA extracted from 103 sheep and 61 goats, amplification with the molecular length 520 base pair was obtained in 67.96% (70/103) and 1.63% (1/61), respectively. In the microscopic examination of thin blood smears, Theileria spp. were observed in 40 out of 103 sheep (38.83%), but Theileria spp. were not seen in goats. The difference between microscopic examination and the PCR results was statistically significant (p < 0.01).
    Turkiye parazitolojii dergisi / Turkiye Parazitoloji Dernegi = Acta parasitologica Turcica / Turkish Society for Parasitology 02/2005; 29(1):17-21.