Ming-Fang Luo

Tsinghua University, Beijing, Beijing Shi, China

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Publications (3)0 Total impact

  • Ming-fang Luo, Hao Wu, Lei Wang, Xin-hui Xing
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    ABSTRACT: From an agricultural sample taken in Chongqing, a stable methane-oxidizing mixed microbial consortium was established by enrichment culture with methane as a sole source of carbon and energy. The mixed consortium showed high capability of phenol degradation and 1,2-epoxypropane production from propene. More than 99% of phenol at an initial concentration of 600mg/L could be degraded by the mixed microbial consortium after 11 h of cultivation. The productivity of 1, 2-epoxypropane could be increased with the decrease of phosphate concentration. The concentration of 1, 2-epoxypropane produced could reach to 5.0mmol/L. The bacterial structure of the methane-oxidizing mixed microbial consortium was analyzed by pure culture isolation combining with 16S rRNA and PCR of the related MMO functional genes. The results showed that the methane-oxidizing mixed microbial consortium was composed of a type 1U methanotroph identified as Methylosinus trichosporium and at least 4 kinds of heterotrophs ( Comamonas testosteroni, Cupriavidus metallidurans, Acinetobacter junii and Stenotrophomonas maltophilia ). M. trichosporium Y9, isolated from the mixed consortium, harbored both sMMO and pMMO genes.
    ACTA MICROBIOLOGICA SINICA 03/2007; 47(1):103-9.
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    ABSTRACT: Effects of organic chemicals on the growth rate and cell density of Methylosinus trichosporium OB3b, a type II methanotroph, were studied. The examined organic chemicals were vitamins, amino acids and organic acids, including folic acid, riboflavin, tetrahydrofuran, cysteine, tyrosine, glycine, pyruvic acid, malate, maleic acid, and citrate. The results showed that M. trichosporium OB3b could not utilize the added organics as the sole carbon source for cell growth without the presence of methane. Riboflavin and organic acids such as malate, citrate, succinate and maleate could obviously improve the cell concentration of this strain. Among them, citrate exhibited the most significant effect on cell growth enhancement. When citrate was added at an optimal concentration of 0.015 mmol/L, the maximal cell concentration could reach 0.75 g dry cell weight/L, about 3.5 times that of the control without citrate addition. Furthermore, the improvement of the cell growth of M. trichosporium OB3b by the addition of citrate was confirmed in a bioreactor with a continuous supply of air and methane.
    Biochemical Engineering Journal. 01/2006;
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    ABSTRACT: Microbial risk in drinking water has attracted more and more attention in recent years. Rapid identification and the behavior of waterborne pathogens in drinking water sources are of significant importance for the effective control of the microbial safety. In this study, the bacteriological quality of the drinking water and the biofilm inside a water purifying filter had been characterized by using heterotrophic plate counts (HPC), commercial API assay kits, scanning electron microscopy (SEM), total direct counts (TDC) and fluorescence in situ hybridization (FISH) approaches. Ten different species of bacteria had been identified by API kits in tap water, eight of which were human pathogens. Correspondingly, six bacterial species were found in the biofilm, and four from the filtered water, which revealed that the filter could reject some species of bacteria in water. And there were four species common in the tap water and the biofilm, which probably pertained to the formation of the biofilm. FISH had been used to confirm the API identification results, showing about 83% consistency. The number of TDC of the tap water was 3.6×10 5 cells/ml, more than 20,000 times of CFU (colony formation unit).

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7 Citations

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  • 2007
    • Tsinghua University
      • Department of Chemical Engineering
      Beijing, Beijing Shi, China