[Show abstract][Hide abstract] ABSTRACT: The Hawaiian Drosophila offer a unique opportunity to examine evolutionary questions because of the known ages of the Hawaiian Islands and the large number of species endemic to this archipelago. One of the more well studied groups of Hawaiian Drosophila is the planitibia species group, a long-standing population genetic model system. Here we present a molecular phylogenetic hypothesis of all 17 taxa in the planitibia group based on nucleotide sequences from two mitochondrial (16S and COII) and four nuclear (Adh, Gpdh, Yp1, and Yp2) loci, accounting for over 4kb of sequence per taxon. We use these data to estimate major divergence times within this group. Our results suggest that the basal diversification within this group, calculated at 6.1 +/- 0.47 MY, predates the oldest high island of Kauai. The older diversifications in this group took place on Kauai, with subsequent colonization and speciation events occurring as new islands became available to Drosophila. Understanding of the phylogenetic relationships of this important group will place the existing population genetic work in a macroevolutionary context and stimulate additional work, particularly on those taxa endemic to the Maui Nui complex of islands.
[Show abstract][Hide abstract] ABSTRACT: In Drosophila melanogaster and the endemic Hawaiian species D. grimshawi three Yolk protein (Yp) genes are expressed in a similar sex- and tissue-specific pattern. In contrast, DNA sequence comparisons of promoter/enhancer regions show low levels of similarity. We tested the functional significance of these observations by transforming D. melanogaster with the genomic region that includes the divergently transcribed D. grimshawi DgYp1 and DgYp2 genes; we found that the introduced genes were expressed in female fat body and in ovaries but not in males. Moreover, we found D. grimshawi proteins in the hemolymph and accumulating in ovaries. Using reporter constructs we showed that the intergenic region from D. grimshawi was sufficient to drive accurate expression, but some low level of ectopic expression was seen in males. Transforming D. melanogaster with constructs bearing deletions within the D. grimshawi intergenic region revealed only subtle effects in the overall level of expression, suggesting a high level of redundancy. Testing mutants in the sex-specific regulator doublesex revealed that it is capable of repressing the DgYp genes in males. Together, these data show that D. melanogaster trans-acting factors can regulate the in vivo pattern of DgYp expression and support the notion of a redundant and complex system of cis-acting elements.
[Show abstract][Hide abstract] ABSTRACT: A new picture-winged Hawaiian Drosophila species from the islands of Kaua'i and O'ahu that is morphologically indistinguishable from Drosophila grimshawi Oldenberg from the Maui Nui islands is described, based on differentiation in ecological, behavioral, cytological, and molecular characters as well as ultrastructural features of the chorion. The new species, D. craddockae, and D. grimshawi represent the first clear case of an allopatric sibling species pair among Hawaiian Drosophilidae (i.e., there is strong evidence for a profound set of intrinsic, genetically determined differences that are not easily diagnosable by the usual morphological methods). Ecologically, D. craddockae is a strict specialist, with oviposition restricted to the decaying bark of Wikstroemia. Drosophila grimshawi, on the other hand, is a generalist that breeds in the decaying parts of 10 families of plants. Data from cytological, behavioral, and molecular analyses are consistent with the geological evidence that species on the older islands are usually more ancestral than those that evolved on the younger islands. Thus, although long-standing ecological theory states that specialization is a derived condition, the biological and genetic evidence all indicate that specialism in D. craddockae is the ancestral condition and that generalism evolved in D. grimshawi on Maui Nui as a derived trait.
[Show abstract][Hide abstract] ABSTRACT: The entomologist R. C. L. Perkins pioneered observations of breeding site ecology for the endemic Hawaiian Drosophilidae, a renowned group of flies that has undergone explosive speciation and adaptive radiation into a wide variety of breeding niches. Females of the various species groups and subgroups oviposit their eggs in either fungi, flowers, fruits, leaves, stems, bark, sap fluxes, or other novel substrates. Varied selective forces in these alternative breeding sites have apparently molded female reproductive characters and strategies into diverse outcomes; some species mature and oviposit only one egg at a time, whereas others oviposit hundreds. Here, we have analyzed the pattern of shifts in breeding substrate, and the associated evolution of selected ovarian, egg, and ovipositor traits, by mapping the various ecological and female reproductive character states on an independently derived phylogenetic hypothesis based on nuclear and mitochondrial DNA sequences. This comparative phylogenetic approach demonstrates a number of strong historical associations among female reproductive traits and between particular traits and the breeding substrate, although the overall pattern is complex and more data are needed. Identification of certain apomorphic traits associated with shifts in breeding substrate suggests an adaptational origin for some of the changes in egg load per fly, in the length of the respiratory filaments of the egg, and in the length and shape of the ovipositor. Although these hypotheses need further testing, it appears that the ecological diversification in breeding substrates has been an integral component in the radiation of drosophilids in Hawai'i.
[Show abstract][Hide abstract] ABSTRACT: The picture-winged species Drosophila grimshawi is unique among Hawaiian Drosophila in its wide geographic range, having populations on several islands of the Hawaiian archipelago. This distribution contrasts with the pattern of single-island endemism observed in most of the picture-winged group; significantly, it does not concur with predictions of the founder theory, where speciation is the typical outcome of founder events involving colonization of a new island. To examine this anomalous situation, we have taken a phylogenetic approach in an attempt to resolve the relationships among taxa and decipher the most probable colonization scenario. We have obtained both morphological and molecular data for all the D. grimshawi populations as well as the closely related species D. pullipes, and two outgroup species, using scanning electron microscopy to score ultrastructural features of the chorion or eggshell, and PCR amplification and nucleotide sequencing to acquire sequence data on Yp1, one of the three Yolk protein genes. In addition, we have used available data on Yolk Protein electrophoretic pattern and jousting, oviposition, and mating behavioral characters. Analyses of these data sets, either individually or in combination, indicate that there are two separate and ecologically distinct clades within this species complex. One clade includes the Kauai and Oahu populations of grimshawi, as well as the closely related species D. pullipes from Hawaii, all of which are classified as ecological specialists with respect to their oviposition and breeding substrate. The other clade includes all the ecologically generalist grimshawi populations of the Maui Nui island complex. The phylogenetic results do not concur with the previously proposed hypothesis that D. pullipes originated from a founder derived from the Maui Nui complex and further suggest that these taxa are in need of taxonomic revision.
[Show abstract][Hide abstract] ABSTRACT: Nucleotide sequence analysis has demonstrated that interspecific size variation in the YP2 yolk protein among Hawaiian Drosophila is due to in-frame insertions and deletions in two repetitive segments of the coding region of the Yp2 gene. Sequence comparisons of the complex repetitive region close to the 5' end of this gene across 34 endemic Hawaiian taxa revealed five length morphs, spanning a length difference of 21 nucleotides (nt). A phylogenetic character reconstruction of the length mutations on an independently derived molecular phylogeny showed clade-specific length variants arising from six ancient events: two identical insertions of 6 nt, and four deletions, one of 6 nt, one of 12 nt, and two identical but independent deletions of 15 nt. These mutations can be attributed to replication slippage with nontandem trinucleotide repeats playing a major role in the slipped-strand mispairing. Geographic analysis suggests that the 15 nt deletion which distinguishes the planitibia subgroup from the cyrtoloma subgroup occurred on Oahu about 3 million years ago. The homoplasies observed caution against relying too heavily on nucleotide insertions/deletions for phylogenetic inference. In contrast to the extensive repeat polymorphisms within other Drosophila and the human species, the more complex 5' Yp2 repetitive region analyzed here appears to lack polymorphism among Hawaiian Drosophila, perhaps due to founder effects, low population sizes, and hitchhiking effects of selection on the immediately adjacent 5' region.
[Show abstract][Hide abstract] ABSTRACT: The endemic Hawaiian drosophilids, a unique group that are remarkable for their diversity and rapid proliferation, provide a model for analysis of the process of insular speciation. Founder events and accompanying random drift, together with shifts in sexual selection, appear to explain the dramatic divergence in male morphology and mating behaviour among these flies, but these forces do not account for their spectacular ecological diversification into a wide array of breeding niches. Although recognized as contributing to the success of this group, the precise role of adaptive shifts has not been well defined.
To delineate the pattern of ecological diversification in the evolution of Hawaiian Drosophila, we generated a molecular phylogeny, using nucleotide sequences from the yolk protein gene Yp1, of 42 endemic Hawaiian and 5 continental species. By mapping ecological characters onto this phylogeny, we demonstrate that monophagy is the primitive condition, and that decaying leaves were the initial substrate for oviposition and larval development. Shifts to decaying stems, bark and tree fluxes followed in more derived species. By plotting female reproductive strategies, as reflected in ovarian developmental type, on the molecular tree, we also demonstrate a phylogenetic trend toward increasing fecundity. We find some statistical support for correlations between ecological shifts and shifts in female reproductive strategies.
Because of the short branches at the base of the phylogram, which lead to ecologically diverse lineages, we conclude that much of the adaptive radiation into alternate breeding substrates occurred rapidly, early in the group's evolution in Hawaii. Furthermore, we conclude that this ecological divergence and the correlated changes in ovarian patterns that adapt species to their ecological habitats were contributing factors in the major phyletic branching within the Hawaiian drosophilid fauna.
Current Biology 11/1995; 5(10):1129-39. DOI:10.1016/S0960-9822(95)00229-6 · 9.57 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Formation of the egg shell (chorion) inDrosophila and Scaptomyza (Diptera : Drosophilidae) is a complex developmental process involving coordinated synthesis and secretion of multiple proteins by the monolayer of follicle cells surrounding the egg. Using scanning electron microscopy, the ultrastructure of the chorion in 37 endemic Hawaiian drosophilids, representing the genera Drosophila and Scaptomyza, were analyzed and compared with 7 representative species of continental Drosophila. The detailed structure of the chorion was described for 8 chorionic regions: the respiratory filaments, follicle imprints, operculum, micropyle, dorsal ridge, ventral rim, posterior pole, and the chorion cross-section. The morphology of each region is similar among related species, but strikingly different among groups. The main functions of the chorion are to protect the developing embryo from the vicissitudes of the environment and to provide channels for gas exchange during embryogenesis. Adaptation to the diverse ovipositional substrates used by Drosophila in general, and the Hawaiian species in particular, has resulted in extraordinary diversity in the various chorionic structures. The respiratory filaments differ in number and have evolved to different lengths and degrees of porosity. Furthermore, other regions also involved in respiratory exchange (the operculum, follicle imprints, the pole region, and the dorsal ridge) have diverged in parallel to the ecological divergence. The thickness and complexity of the outer endochorion are dramatically different in various groups, providing varying degrees of mechanical strength to the eggshell, which promotes embryonic survival in the diverse microenvironments. These varied chorionic structures have been found to provide useful morphological characters for phylogenetic analyses of the drosophilids.
International Journal of Insect Morphology and Embryology 04/1993; 22(2-4-22):417-446. DOI:10.1016/0020-7322(93)90022-S
[Show abstract][Hide abstract] ABSTRACT: In 35 Hawaiian Drosophilaspecies collected from natural populations, all reproductively mature females were inseminated. Onset of receptivity to insemination broadly correlated with the vitellogenic stage of ovarian development, but species and species groups varied in the exact stage of vitellogenesis at which earliest insemination took place. A striking exception was observed in two picture-winged species of the adiastolasubgroup, which were precociously inseminated in previtellogenesis, close to the time of adult eclosion. For a given species, the range of ovarian stages from earliest receptivity to insemination of all females, termed the “insemination period,” also varied among species. This variability may be due to physiological variation between females in hormonal levels, low population densities, and lek behavior of males, or it may reflect variation in degree of female discrimination toward male courtship attempts. Consideration of the quantities of sperm observed stored in the female sperm storage organs, relative to the numbers of eggs produced, indicates obligatory multiple insemination for some species, notably the fungus breeders. In other species groups, there is no apparent necessity for remating, although it may occur.
[Show abstract][Hide abstract] ABSTRACT: Egg and female hemolymph proteins were resolved via SDS-polyacrylamide gel electrophoresis in a diverse array of 33 endemic Hawaiian drosophilids, encompassing 17 picture-winged species, 3 of the antopocerus species group, 9 fungus feeders, 1 species from each of the modified mouthparts, crassifemur and ciliated tarsus groups, and 1 Scaptomyza species. Molecular weights of the two (10 species) or three vitellogenin bands (22 species) were highly variable, spanning a 7-kD range. The largest vitellogenin, V1, was the most variable, showing a change of some 10% in its mean size of 47.6 kD. The smallest V3 vitellogenin, mean size 44.1 kD, was evolutionarily the most conservative in size. The species Drosophila hawaiiensis was found to be polymorphic for two/three vitellogenin bands and, also, polymorphic with respect to the size of the V1 protein. No inter- or intrapopulation variability in vitellogenin size was detected in 10 other species examined. The major features of vitellogenin protein evolution in the Hawaiian Drosophila are change in molecular weight and regulatory differences that result in quantitative differences between species in patterns of vitellogenin protein production.
[Show abstract][Hide abstract] ABSTRACT: The temporal pattern of protein production and, in particular, vitellogenin protein synthesis during the sexual maturation of Drosophila grimshawi females has been studied in vivo by briefly feeding the flies with 35S-methionine and 3H-amino acids. The overall level of incorporation was very low in young flies; it then progressively increased to reach a maximum with the onset of sexual maturity at 13-15 days. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analyses revealed three classes of proteins: those synthesized throughout the age spectrum, which constitute the majority of protein species; proteins synthesized primarily or only in young flies; and proteins synthesized only by the older flies. In this Drosophila species, the three vitellogenins (V1, V2, and V3) appeared to be synthesized in a two-phase pattern. In the first phase, small quantities of V1 and V2 were detected immunologically in the fat body and hemolymph of newly emerged and 1 day-old flies. These proteins did not accumulate in the hemolymph or the ovaries, apparently being unstable proteins. The second phase commenced in early vitellogenesis (7-9 days of age) with synthesis in the fat body of small quantities of V1 and V2, followed by V3 proteins. These proteins were secreted and accumulated in the hemolymph and 24 h later were found in the ovaries. Their quantities increased rapidly and a steady state of synthesis, release into the hemolymph, and uptake by the ovaries was reached by days 13-15. We have estimated that during the steady state of vitellogenin synthesis, a fly can synthesize in 24 h at least 152 micrograms of vitellogenins, which is more than 2% of its body weight, at an average rate of about 6.3 micrograms vitellogenins/h. About 2 micrograms of this are synthesized in the fat body, and about 4 micrograms in the ovaries. These findings are discussed in terms of their physiological implications and contrasted with the available data on Drosophila melanogaster.
Journal of Experimental Zoology 09/1989; 251(3):339-48. DOI:10.1002/jez.1402510310
[Show abstract][Hide abstract] ABSTRACT: Two V3 vitellogenin clones isolated from genomic libraries ofDrosophila grimshawi (G1, Auwahi, Maui) were found to differ in length. Structural comparison of the two clones established that the length difference could be attributed to two insertions/deletions of about 200 bp each, both within the 3 flanking sequences of the gene. The two length variants appeared to be polymorphic in the G1 laboratory strain, as demonstrated by analysis of genomic DNA isolated from single flies. The deleted variant sequence was traced by further analysis to two otherD. grimshawi strains (PK9 and S10G1) which originated from the island of Molokai. The existence of this morph in the Maui strain appears to have resulted from a laboratory stock contamination at the Drosophila Stock Center. In the course of a few generations of culture of this G1 strain at New York University, the deleted morph increased its frequency surprisingly rapidly, almost replacing the original morph, while at the Bowling Green Stock Center, the original morph still predominates. These frequency changes are most likely consequences of genetic drift due to bottlenecks in the maintenance and propagation of this stock.
[Show abstract][Hide abstract] ABSTRACT: 1. The three female-specific vitellogenin proteins, namely V1, V2 and V3, have been isolated and characterized from Drosophila grimshawi. Their mol. wt, as determined by SDS-polyacrylamide gel electrophoresis are 46,000, 45,000 and 43,000 which are in agreement with those determined by Ferguson plot analysis. 2. All three vitellogenins appear to be monomers in the ovarian extracts and they have very similar biochemical and immunological properties. 3. Ion-exchange chromatography, double immunodiffusion tests and partial digestion with Staphylococcus aureus V8 protease indicated more physicochemical and structural similarities between the V1 and the V2 polypeptides. 4. The distribution pattern of the proteolytic polypeptides resulting from limited chymotrypsin digestion suggested partial homology in the primary structure of the three vitellogenin proteins.
[Show abstract][Hide abstract] ABSTRACT: The three vitellogenin transcripts from Drosophila grimshawi have an average size of 1,600 nucleotides as determined using denaturing electrophoretic conditions. Southern analysis showed that the large quantity of vitellogenin mRNAs in adult female fat body cells is not a reflection of specific gene amplification. The quantitative differences in mRNA accumulation between fat body and follicle cells, which are in concert with the onset of their translation, indicate that vitellogenin synthesis entails the regulated expression of individual genes. The expression of the vitellogenin genes during follicle development is stage specific: V1 and V2 expression starts at late stage 7, while V3 is delayed by one stage. Maximum transcription of all three genes occurs at stage 10 whereas at stage 12 none of the transcripts is present. These results suggest that, either there is more than one regulatory signal, or there is one to which each gene reacts differently. Surprisingly, in male fat body cells a V2 transcript has been detected which is also present in the poly(A)+RNA fraction: the function and the purpose of this particular vitellogenin mRNA in male fat body cells are unknown. Neither of the other two vitellogenin transcripts have been detected in male fat body cells.
MGG - Molecular and General Genetics 11/1987; 210(3):564-571. DOI:10.1007/BF00327213
[Show abstract][Hide abstract] ABSTRACT: We isolated recombinant genomic DNA clones containing sequences coding for the female-specific vitellogenin proteins of Drosophila grimshawi. By screening with cDNA vitellogenin clones derived from female fat body mRNA we were able to isolate all three genes, namely V1, V2 and V3. The identity of these genes was established first by cell-free translation of the hybrid-selected mRNA followed by protease digestion of the in vitro translation products and second by hybridization of the three genes to electrophoretically separated mRNAs. The transcriptional orientation of these genes was determined. The V1 and V2 genes have opposite orientations with their 5-ends 1.75 kb apart. S1 analysis demonstrated that the V1 gene has three exons of 310, 400 and 980 bp in length and two introns of about 120 bp. The V2 gene has two exons of 300 and 1260 bp in length and an intron 100 bp long. The V3 gene has three exons of 250, 375 and 820 bp in length and two introns of about 120 bp. The homology, in both sequence and structure, of the vitellogenin genes indicates that they have arisen by duplication events from an ancestral gene. Moreover, the similarity of the V1 and V2 gene positions within the genome of the two distant species D. melanogaster and D. grimshawi suggests a functional coupling of these two genes during vitellogenin gene expression.
MGG - Molecular and General Genetics 02/1987; 206(3):475-484. DOI:10.1007/BF00428888
[Show abstract][Hide abstract] ABSTRACT: In analyzing the in vitro pattern of protein synthesis by the fat body and ovaries of the Hawaiian species Drosophila grimshawi, we have found that the ovaries synthesize much more protein than the female fat body and that the majority of the synthesized proteins are retained by the ovarian tissues. In contrast, the fat body secrets most of the proteins into the culture medium. Vitellogenins are the major class of proteins synthesized and released into the medium by both tissues. The synthesis of the three vitellogenin proteins (V1, V2, V3) is noncoordinate in the two tissues. Ovaries synthesize much more of the V2 protein, less V1 and very little V3, whereas fat body synthesizes more V1 protein with lesser quantities of the other two. The follicle cells were identified as the site of ovarian vitellogenin synthesis in D. grimshawi, confirming the findings in D. melanogaster. In D. grimshawi, the three vitellogenins are synthesized by the follicle cells in a noncoordinate and developmentally regulated manner. V2 and V1 are the predominant proteins at the onset of vitellogenesis (S8-9); their production peaks together with that of V3 a few hours later (S10) and then decreases to quantities equal to that of V3 during early choriogenesis (S11). During active choriogenesis (S12), V2 and V1 cease to be synthesized, but V3 synthesis continues. The vitellogenins synthesized by the follicles in vitro are released into the medium and not incorporated into the oocyte.
[Show abstract][Hide abstract] ABSTRACT: The eggshell of the Hawaiian speciesDrosophila grimshawi was analyzed in terms of its structure and biochemical composition. It is generally similar toD. melanogaster in radial structure except that the inner endochorion is relatively compact and the outer endochorion is thick (1.9 m) and traversed by numerous 50-nm-wide aeropyles (not present inD. melanogaster). Regionally the eggshell consists of the various specialized regions, which are: the holey posterior pole; the dorsal ridge; the operculum with very tall imprint borders; the collar with discontinuous outer endochorion; the micropyle with an external eccentric hole and an internal eccentric paracrystalline region; and the four very long respiratory appendages which exhibit tightly covered surface features for underwater plastron respiration. On the basis of the above structural data we suggest that the entireD. grimshawi eggshell (various layers and specialized regions) is the product of at least eight follicle cell subpopulations. In addition, in vitro culturing with3H-proline indicates that the eggshell is made according to a strict developmental program (analogous toD. melanogaster) and consists of several proteins ranging in molecular weight from 14,000 to 37,000 daltons.
Development Genes and Evolution 10/1983; 192(6):303-316. DOI:10.1007/BF00848810 · 2.44 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: The three major yolk proteins (YP-1, YP-2, YP-3) of Drosophila melanogaster, have been isolated from mature eggs. Their molecular weights as determined by SDS-polyacrylamide gel electrophoresis, were found to be for YP-1, YP-2, and YP-3, respectively. Their molecular weights, as determined by gel filtration on Sephadex G-200, were found to be 4.7 × 104 for YP-1 and YP-3, and 13.5 × 104 for YP-2. Their isoelectric points, as determined by isoelectric focusing on polyacrylamide gels, were found to be 6.5, 6.65 and 6.9 for YP-1, YP-2 and YP-3, respectively. Amino acid analysis, indicated peptides rich in acidic amino acids with substantially lower levels of basic amino acids. Lysine, aspartic acid, and serine were identified as the residues of YP-1, YP-2 and YP-3, respectively. YP-2 was found to contain 7.5% carbohydrates while no detectable amounts of carbohydrates could be found in YP-1 and YP-3. The data reported here indicate pronounced similarities between these three proteins.
[Show abstract][Hide abstract] ABSTRACT: The rare O'ahu picture-winged fly Drosophila ambochila Hardy & Kaneshiro is endemic to two windward ravines in the Wai'anae Mountains that harbor its host plant. Drosophila ambochila is an ecological specialist that breeds on Pisonia stems and trunks in an intermediate stage of decay. By providing field-collected females with suitable substrate material, we have been able to observe the oviposition behavior of this species in the laboratory and obtain F 1 larvae. In nature, females oviposit each batch of mature eggs ("'4050) in a single cluster, by repeatedly inserting their long ovipositor into the same crack or beetle hole in the decaying Pisonia bark. Ovipositor, ovary, and egg morphology are characteristic of bark-breeding Hawaiian Drosophila, but SEM studies revealed a distinctive chorionic ultrastructure for the eggs of this species. Larval salivary chromosome analyses indicated that the O'ahu D. ambochila is most closely related to D. alsophila from the island of Hawai'i and have helped to resolve the phylogenetic relationships among six of the nine species belonging to the vesciseta subgroup of the glabriapex species group.