Are you Mohammad H. Abnosi?

Claim your profile

Publications (14)17.78 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: A new asymmetric heptaaza Schiff base macrocyclic bis(pendant donor) manganese(II) complex, [MnL(1)](ClO(4))(2).CH(3)CN (1), has been prepared and characterized by X-ray diffraction and spectroscopic methods. The antimicrobial activity of 1 and a series of its familiar symmetric heptaaza [15]pydieneN(5), [16]pydieneN(5), and [17]pydieneN(5)-based bis-(2-aminoethyl) pendant armed Schiff base macrocyclic complexes of Mn(II) were tested against Escherichia coli, Staphylococcus aureus and Candida albicans. The results showed that the symmetric heptaaza [16]pydieneN(5), and [17]pydieneN(5)-based Schiff base macrocyclic complexes of Mn(II) had remarkable inhibition zone on the culture of S. aureus and E. coli as compared with standard drugs. The optimized geometry of the prepared complex has been obtained from density functional method, DFT, using B3LYP/6-31G* basis set.
    Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy 10/2010; 77(2):342-7. · 1.98 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: This study was organized to see whether vitamin E, as a strong antioxidant, could affect the abnormalities of testis structure caused by para-nonylphenol (p-NP) during its development. A total of 32 female Wistar rats after mating were divided into four groups (n = 8): control, vitamin E (100 mg kg(-1) per day), p-NP (250 mg kg(-1) per day) and p-NP + vitamin E. The rats were treated from the seventh day of pregnancy till the twenty-first day. After weaning, the male pups were divided into the same groups and were treated orally for 90 days. Finally, the right testis was fixed, processed, stained and studied using stereological methods. The weight and volume of testis, volume of seminiferous tubules and its diameter, thickness of the basement membrane, height of the germinal epithelium, total number of types A and B spermatogonia, spermatocyte, spermatid and Sertoli cells were significantly reduced in p-NP group when compared with other groups. Co-administration of vitamin E and p-NP compensated for the adverse effects of p-NP on the above parameters. In addition, treatment with only vitamin E caused a significant increase in diameter, basement membrane thickness and height of germinal epithelium, number of spermatogonia and spermatocytes. Co-administration of vitamin E with p-NP could prevent the adverse effects of p-NP on the testis structure during its development.
    Asian Journal of Andrology 08/2009; 11(4):508-16. · 2.14 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Two new pendant armed Schiff base macrocyclic complexes, [MgL1](ClO4)2 (1), and [MgL2](ClO4)2 (2), have been prepared via cyclocondensation of 2,6-diformylpyridine and 2,6-diacetylpyridine with two hexadentate hexaamines, ten and tmen, in the presence of Mg(II) ion. The ligands are 15-membered pentaaza macrocycles having two 2-aminoethyl pendant arms. The newly prepared complexes are investigated by IR, 1H NMR, 13C{1H} NMR, DEPT(135), COSY(H, H) and HMQC spectroscopic methods. The antimicrobial screening of newly prepared complexes, 1 and 2, as well as previously prepared similar complexes, [MgL3](ClO4)2 (3) and [MgL4](ClO4)2 (4), against Escherichiacoli, Staphylococcusaureus and candidiaalbicans showed that the macrocyclic complexes of Mg(II) containing 15-membered pentaaza ring (1, 2 and 3) have no activity. Where as the compound 4, which contain 16-membered pentaaza ring, had remarkable inhibition zone on the culture of S.aureus and E.coli as compared with standard drugs. The 1H and 13C chemical shieldings of gas phase complexes were also studied by the gauge independent atomic orbital (GIAO) and continuous set of gauge transformations (CSGT) methods at the level of density functional theory (DFT). The computed 13C chemical shifts are in reasonably good agreement with the experimental data.
    Journal of Inclusion Phenomena 01/2009; 63(1):97-108. · 1.40 Impact Factor
  • Alireza Salabat, Mohammad H. Abnosi, Ahmad Motahari
    [Show abstract] [Hide abstract]
    ABSTRACT: The partitioning behavior of three amino acids, l-tryptophan, l-phenylalanine, and l-tyrosine, has been studied in aqueous two-phase systems of polyethylene glycol (PEG6000) + salts + H2O at 298.15 K. The salts used were magnesium sulfate, sodium sulfate, and ammonium sulfate. The effects of tie line length, salt, and side chain structure on the partition coefficients of amino acids have been studied. The results showed that increasing amino acid hydrophobicity and tie line length led to a corresponding increase of the partition coefficients. In addition, we also showed that the partition coefficients of the amino acids in the systems containing Na2SO4 are also significantly greater than the other two salts. The experimental data are correlated using a modified virial-type model. Comparisons between model and experimental data reveal a good agreement.
    Journal of Chemical and Engineering Data - J CHEM ENG DATA. 08/2008; 53(9).
  • [Show abstract] [Hide abstract]
    ABSTRACT: A series of new Schiff base hydrazones (compounds 1-16) were synthesized by condensation reaction of 4-amino-3-(4-pyridine)-5-mercapto-1,2,4-triazole with various aldehydes and/or dialdehydes. The structure of the prepared compounds was confirmed by means of 1H NMR, 13C NMR, UV-vis, IR and elemental analyses. The all prepared compounds were assayed for antibacterial (Escherichia coli and Staphylococcus aureus) and antifungal (Candida albicans) activities by disc diffusion method. The results indicate that all tested compounds did not show any antibacterial activity against E. coli, as gram negative bacteria, and antifungal activity against C. albicans. But the compounds 2, 3, 4, 6 and 8 containing 4-Cl, 4-Me, 4-MeO, 2,4-di-Cl and 2-OH substituted phenyl moiety, respectively, showed good inhibition against S. aureus as compare to standard drugs. The structure of all biologically active compounds has also been theoretically studied by ab initio Hartree-Fock (HF) methods.
    Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy 06/2008; 71(4):1474-80. · 1.98 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The aim of this study was to investigate the short term effect of aqueous-ethanolic extract of garlic in preventing and curing nephropathy following induced diabetes in rats using streptozotocin (STZ), with the help of stereological methods. Thirty two male wistar rats were randomly divided into control, control+extract, diabetic and diabetic+extract groups (n = 8). Diabetes was induced by intraperitoneal injection of 60 mg kg<sup>-1</sup> of streptozotocin (STZ). Treatment with aqueous-ethanolic extract of garlic (50 mg kg<sup>-1</sup> day<sup>-1</sup>) was followed for four weeks, then the left kidneys were excised and stereological studies were carried out. The results revealed that blood sugar of the diabetic rats was reduced significantly following treatment with garlic extract. The final body weight of both diabetic and diabetic+extract groups didn`t increase significantly, but in control and control+extract groups the body weight increased significantly (p<0.001). A significant increase in the kidney weight of the diabetic and diabetic+extract rats was found (p<0.001) compared to the control ones. The volume of cortex, medulla and total volume of kidney increased significantly in the diabetic and diabetic+extract groups compared to the control ones. The total glomerular volume increased significantly in diabetic rats compared to the other groups (p<0.05). In conclusion the aqueous-ethanolic extract of garlic influences the volume of total glomeruli but has no effect on the volume of other kidney components.
    Journal of Biological Sciences. 01/2008;
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Organotypic spinal cord slices from neonatal mammals could be a powerful model for evaluation of cell survival but also cell death mechanisms. The aim of this study was to establish an in vitro model for investigating cell survival and mechanism involved in cell death in neonatal spinal cord slices. The spinal cord was sliced and incubated into culture medium. The MTT assay was carried out to assess the viability of the slices and fluorescent staining was used to study morphological features of apoptosis, where as nucleosomal DNA fragmentation was detected using agarose gel electrophoresis. The results of the present study demonstrated that the slices could be maintained in culture up to 14 days. Both neurons and glial cells died by apoptosis and application of a general caspase inhibitor neither affected slice survival nor nucleosomal DNA fragmentation after 24 h in culture. In addition, the inhibitor failed to block apoptosis in neurons and glial cells in the cultured slices. Our results suggest that in the cultured slices, apoptosis is the main reason for neuron and glial cell death, which occurs by a caspase-independent mechanism.
    Iranian Journal of Science & Technology, Transaction A. 01/2008; 32.
  • Alireza Salabat, Mohammad H Abnosi, Azadeh R Bahar
    [Show abstract] [Hide abstract]
    ABSTRACT: The counter-current chromatography method using aqueous two-phase systems, which is a form of liquid-liquid partition chromatography, could be applied for separation of the amino acids. This method needs some information about the partition coefficient of the amino acids in such systems. In this work, partitioning of amino acids d-alanine, l-valine and l-leucine was investigated in aqueous two-phase system of polypropylene glycol (PPG425)+MgSO4+H2O at 298.15K. The results showed that increasing the amino acid hydrophobicity lead to a corresponding increase in the partition coefficients and increasing tie line length lead to decreasing partition coefficients. The effect of the pH on amino acids partitioning was also determined. The experimental data are correlated using a modified virial-type model. The comparisons between the correlation and the experimental data reveal a good agreement.
    Journal of Chromatography B 11/2007; 858(1-2):234-8. · 2.49 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Eight new heterodinuclear Cu(II)–M(II) (M = Pb and Zn) complexes of four new phenol based compartmental macrocyclic ligands, possessing contiguous (N2O2) and (NxO2) (x = 2, 3) coordination sites, were prepared by the template reaction of [N,N′-bis(3-formyl-5-methylsalicylidene)ethane-1,2-diaminato]copper(II), with various di- and/or tri-amines in the presence of Pb(II) and Zn(II) ions. The crystal structure of [CuZnL3(H2O)](ClO4)2, 6, was determined by X-ray diffraction and shows that the Zn(II) and Cu(II) ions reside in the N2O2 sites of the macrocyclic ligand. The fifth coordination site of the Zn centre is occupied by a water ligand. All the complexes have been characterized by elemental analysis, molar conductivity and spectroscopic methods (IR and UV). Also, all the synthesized complexes were screened for their antibacterial and antifungal activity against Escherichia coli, Staphyloccocus aureus and Candida albicans.
    Polyhedron 10/2007; 26(17):4963–4970. · 2.05 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The aim of this study is to investigate the preventing effect of wheat germ oil as a rich source of vitamin E on the serum biochemical factors in the rats exposed to para-nonylphenol. Four groups (n = 6) of male Wistar rats was orally given para-nonylphenol (200 mg kgG<sup>-1</sup>dayG<sup>-1</sup>) for 70 days. After treatment the blood samples were obtained and LH, FSH, testosterone, progesterone and estrogen, transaminases, phosphatases, LDH, triglyceride, cholesterol, HDL-cholestrol and protein analysis were carried out. Para-nonylphenol caused a significant increase in LH level where other hormones remained unchanged. In addition a significant reduction was found in AST, ALT and LDH level while alkaline phosphatase increased significantly following treatment with para-nonylphenol. There was no change in the level of lipids and proteins in this study with respect to para-nonylphenol treatment. Co-administration of wheat germ oil with para-nonylphenol compensated the imbalance of the LH, AST, ALT and LDH caused by para-nonylphenol to the control level. However treatment of the rats with only wheat germ oil caused a significant reduction in the level of the hormones except for progesterone. Administration of wheat germ oil in any case caused a reduction in triglyceride level. Co-administration of wheat germ oil with para-nonylphenol eliminated the effect of para-nonylphenol on LH hormone as well as enzymes. It seems that daily used of wheat germ oil may have some benefits to the para-nohnylphenol exposure, however more clinical studies are needed to find more information.
    Journal of Biological Sciences 08/2007;
  • [Show abstract] [Hide abstract]
    ABSTRACT: The aim of this study was to investigate the growth and survival rate of preantral follicles isolated from vitrified ovarian tissue by Cryotop and conventional methods. The ovaries of 14-day-old mice were separated and divided into four groups as following: Cryotop group, vitrified by Cryotop; CV (Conventional; CV) group, vitrified by conventional straw; toxicity test group and control group. After warming the vitrified ovaries, isolated preantral follicles from four groups were cultured for 4 days to compare survival rate and follicular growth between above-mentioned groups. Survival rate (97.3%) in toxicity test group alike the control group (98.7%) were significantly higher (P<0.05) than the Cryotop (92.7%) and CV (47.7%) groups. Increase in follicle diameters after 4 days in Cryotop and CV groups was significantly lower (P<0.05) than the control and toxicity test groups, but growth and survival rate of follicles in Cryotop group was significantly higher (P<0.05) than the CV group. These results demonstrated that ovarian tissue vitrification by Cryotop highly preserves the viability rate of preantral follicles.
    BioFactors 01/2007; 31(2):117-26. · 3.09 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: In this investigation, blood sugar and the kidney histopathological changes in diabetic rats were studied stereologically after being treated with the aqueous ethanol extract of Medicago sativa . Thirty two male Wistar rats were randomly divided into control, control+extract, diabetic and diabetic+extract groups (n=8). Diabetes was induced by intraperitoneal injection of 90 mg/kg of streptozotocin. Treatment with aqueous ethanol extract of Medicago sativa (550 mg/kg) was followed for 4 weeks. Then the left kidneys were excised and stereologically studied. The results revealed that blood sugar of the diabetic rats was reduced highly significant following treatment with aqueous ethanol extract of Medicago sativa . The final body weight in the diabetic, control and control+extract rats increased significantly in comparison with the first body weight but not in the diabetic+extract group. The kidney weight was the same in all groups except for the diabetic+extract rats which increased significantly compared to the control+extract ones. The total volume of kidney and the volume of cortex were the same in all groups while the volume of medulla increased significantly in the diabetic+extract groups compared to the control+extract ones. In addition the total glomerular volume increased significantly in diabetic rats compared to the control ones. In conclusion, although the consuming dose of the extract of Medicago sativa caused a noticeable reduction of blood sugar; however, it had no distinct effect on nephropathy side effects in this short term study.
    Indian Journal of Pharmaceutical Sciences. 01/2007;
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Arsenic salts like sodium arsenite and/or vasectomy may cause variation in sex hormones and enzymes involved in reproductive systems. Four groups of rats (vasectomy + sodium arsenite), (vasectomy), (sham + sodium arsenite) and (sham only) were considered and 8 mg/kg/body weight/day of sodium arsenite was given for a period of 64 days to the treated rats. Hormones (FSH, LH and testosterone), enzymes (aspartate transaminase, alanine transaminase and alkakinephosphatase) and electrolytes (Sodium and potassium) were evaluated using standard methods then the results analyzed using one way ANOVA. The significant reduction of testosterone level was observed in vasectomy + sodium arsenite group compared to vasectomy (p<0.05) and sham only (p<0.001) groups. Level of the LH was decreased significantly (p<0.05) in sham + sodium arsenite and vasectomy groups, when compared to sham only group. In vasectomy group treated with sodium arsenite level of ALP and AST enzymes were decreased significantly (p<0.05) as compared to sham only group. Also level of the AST was decreased significantly (p<0.05) in vasectomy group compared to sham only group. Vasectomy and sodium arsenite toxicity affect the normal level of AST, alkalinephosphatase, LH and testosterone in the rats. A cumulative effect was observed when Vasectomised rats were exposed to sodium arsenite.
    International Journal of Pharmacology 05/2006; · 1.20 Impact Factor
  • A. R. Salabat, M. H. Abnosi, A. Motahari
    [Show abstract] [Hide abstract]
    ABSTRACT: Partitioning of Bovine serum albumin (BSA), β-lactoglobulin (β-LG) and zein as model proteins in aqueous two-phase systems (S) containing polypropylene glycol (PPG425) or polyethylene glycol (PEG 6000) and salts (MgSO4, (NH4)2SO4, Na2SO4) is presented in this paper. The effects of different factors such as tie-line length, salt type and polymer type on the partition coefficient and recovery percent of proteins were analysed. The model proteins were separated by these systems (S) and directly used for gel electrophoresis without separating the target proteins from phase-forming reagents. The results revealed that the S, studied in this work could be used as a novel prefractionation method in proteomic analysis and could separate proteomic proteins in multigroup by one step extraction.
    Journal of the Iranian Chemical Society 7(1). · 1.47 Impact Factor