ABSTRACT: To describe the prevalence and risk factors of hepatitis C virus (HCV) occurred in Henan province, at the end of 2011.
Five villages round Maqiao township, including 5187 residents, were selected for the study. Five-milliliter-blood was drawn from every one of the interviewee. Clinical data including age, gender and anti-HCV antibody was recorded. Patients with positive antibodies against HCV were tested for HCV RNA.
A total number of 5187 people from five villages were studied, with age span from 1 to 97. The average age was 48 years and the sex ratio was 1:1.34. The anti-HCV result showed that the prevalence was 2.27%, with 1-9 age group the lowest (1.55%) and the ≥ 50 year-olds the highest (4.93%). Different villages seemed to have significant differences on the prevalence of HCV, with the highest as 8.68% and the lowest as 0.55%. Under risk factors analysis and distance-infection rates linear regression analysis, data showed that the prevalence might have correlated to the behavior of a certain family-run clinic.
from multivariate analysis indicated that factors as intravenous dropping, intravenous injection and the use of surgery/endoscope were associated with the HCV infection in this village.
Although the public health care system had been developed for more than ten years, iatrogenic infection was yet responsible for the infection of HCV patients in the rural areas of China that called for further attention paid to the system.
Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi 07/2012; 33(7):722-5.
Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi 06/2010; 31(6):712-3.
ABSTRACT: To investigate the epidemiological, genealogic characteristic, familial history of the families with fatal familial insomnia, its clinical and pathological features as well as the heredity rule of related genes.
135 familial members of 7 eras were studied. Vein blood samples from patients as well as from some familial members were collected. PRNP gene was studied with PCR, its serial was determined and then authenticated with Nsp I . Brain tissue was obtained for neuropathological test and PrP(Sc) test with Western blot method.
Clinical symptoms of the 2 diagnosed cases were typical. 11 familial members died of similar neural disease. 32 samples of their familial members, codon at D178N of PRNP of 11 members was mutated, with mutation rate as 34.38% while D129N showed as methionine. Brain tissue of both probands denaturalized into spongiform and the nerve fiber was absent but PrP(Sc) protein was identified.
Genealogy was described in the family with fatal familial insomnia since the patients had typical clinical symptoms and pathological characteristics. It seemed necessary to confirm cases of fatal familial insomnia and their genealogy with epidemiological data and to investigate its gene characteristics as well as with neuropathological and Western blot tests.
Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi 02/2009; 30(1):1-5.
ABSTRACT: To understand the epidemic status of Rickettsia in Xinyang areas of Henan province.
Samples including liver, spleen, kidney from mouse and chigger mites from Xinyang areas and serum samples were detected by nested-polymerase chain reaction (PCR) and indirect immunofluorescence assay (IFA).
In 62 viscus samples from mice organs, the positive rates were 16.13%, 8.06% and 6.45% for Orientia tsutsugamushi, R. typhii and Spotted fever group rickettsiae respectively. In blood clots samples from mice, the positive rates were 8.06%, 6.45% and 1.61 % for O. tsutsugamushi, R. typhii and Spotted fever group rickettsiae respectively. Three out of 26 mouse serum samples were positive for the predicted fluorexcent intensity O. tsutsugamushi.
Using nested-PCR and IFA methods, O. tsutsugamushi, R. typhii and Spotted fever group rickettsiae were detected in the captured mice living in Xinyang areas of Henan province. Results showed that there were intensive natural reserviors of Rickettsia in Henan province, suggesting that the risk of outbreak of Rickettsia in these areas was high.
Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi 07/2007; 28(6):547-9.