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ABSTRACT: BACKGROUND: The distribution and characterization of OXA-type carbapenemases in Acinetobacter sp in Taiwan has less been reported. The aim of the study was to investigate the molecular epidemiology and OXA-type carbapenemase genes in a regional hospital in Taiwan. METHODS: Imipenem-resistant Acinetobacter sp were collected between 2005 and 2007 in a regional hospital. Genotyping was performed by pulsed-field gel electrophoresis. OXA-type carbapenemase genes were determined by multiplex PCR and gene sequencing. RESULTS: A total of 136 isolates were collected. Fifty-six pulsotypes were identified. None of the pulsotypes established predominance throughout the 3-year period. Multiplex PCR of bla(OXA) genes showed that 99% (135/136) of the Acinetobacter sp possessed bla(OXA51-like) genes. The coexistences of bla(OXA51-like)/bla(OXA-23-like) and bla(OXA51-like)/bla(OXA-24-like) were detected in 19% (26/136) and 1% (2/136) of the isolates, respectively. Among bla(OXA-23-like) gene-carrying isolates, two isolates (Pulsotypes 18 and 20) were found in 2006 and the remainder (n=24), including Pulsotypes 27 (n=18), 29 (n=1), 52 (n=3), and 53 (n=2), were found in 2007. Sequencing performed on the 26 representative isolates confirmed the presence of the bla(OXA-23) carbapenemase gene. Analysis of the genetic content of bla(OXA-23) showed that these genes were presumably chromosomal and associated with the upstream-located insertion sequence ISAba1. CONCLUSIONS: The emergence and imminent widespread of bla(OXA-23)-carrying imipenem-resistant Acinetobacter sp appeared in Taiwan during the period from 2006 to 2007.
Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi 01/2011; 44(1):39-44. · 1.63 Impact Factor