[show abstract][hide abstract] ABSTRACT: The effects of various estrogen replacement protocols to prevent bone loss following ovariectomy have been the subject of many studies in rats. This study was designed to determine the effects of early intermittent high-dose estrogen replacement therapy, which has hitherto not been studied, on bone structure of ovariectomized rats.
Bilateral ovariectomies were performed in 20 female mature non-pregnant Wistar rats. All the animals were randomly assigned to two groups to receive either subcutaneous 17 beta-estradiol (25 mg/kg) or only sesame oil on days 15 and 22 after ovariectomy. Fourteen days after the last injection, the rats were sacrificed and proximal femurs were removed for both light and electron microscopic analyses.
In the light microscopic analysis, control femurs exhibited a marked destruction in the structure of the cancellous bone, whereas estradiol-treated rats had almost normal cancellous bone. Ultrastructural analysis showed degeneration and increased turnover in bone cells of the control femurs, whereas the bone cells and the bone matrix appeared almost normal in the treatment group. A statistically significant increase in serum estrogen levels was found in estradiol-treated rats (580+/-124 pg/ml versus 62+/-16 pg/ml, p<0.001).
Intermittent high-dose estrogen treatment prevents cancellous bone loss in the proximal femurs of ovariectomized rats through inhibition of bone turnover and results in significantly increased serum estrogen levels.
[show abstract][hide abstract] ABSTRACT: It is known that Adriamycin, which is widely used in the treatment of various neoplastic conditions, exerts toxic effects in several organs. In this study, we have established that vitamin E has some beneficial effects on the kidney by protecting it from some of the toxicity induced by Adriamycin. A study was carried out which comprised one control group and two experimental groups of guinea pigs. In the experiment Adriamycin was administered either alone (group II) or together with vitamin E (group III). The results of groups II and III were compared with controls (group I). The kidneys were subsequently removed and examined by routine electron microscopic techniques. We found that vitamin E administered together with Adriamycin could reverse some of the degenerative changes caused by Adriamycin.
[show abstract][hide abstract] ABSTRACT: SUMMARY The aim this study was to determine the presence and localisation of insulin like growth factor-I (IGF-I) which had inducable effect on fibroblast proliferation and collagen synthesis, in diabetic and nondiabetic rats' wound healing process, by using comperative immunohistochemical techniques. In the study, Wistar type rats were randomly divided into control and experimental groups. 60 mg / kg Streptozotocine that was solved in 0,9% NaCl solution was administered intraperitoneally as a single dose in the experimental group. Two days later, the plasma glucose level was measured in the blood obtained from tail vein. If the blood glucose level was 15 mmol/L and more, the group was then called the diabetic group. 4cm full thickness skin incision was done in both groups, under ether anesthesia. Immunohistochemical staining was made to the samples, obtaind from the wound on the 1st, 3rd, 10th and 14th days. ILGF-1 appears later in the diabetic wound tissue, when compared with normal wound tissue. This was considered to be one of the reasons that delays wound healing in diabetes.