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Bin Li,
Haofei Liu,
Kongwang He,
Rongli Guo,
Yanxiu Ni,
Luping Du, Libin Wen,
Xuehan Zhang,
Zhengyu Yu,
Junming Zhou,
Aihua Mao,
Lixin Lv,
Yiyi Hu,
Yang Yu,
Haodan Zhu,
Xiaomin Wang
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ABSTRACT: A field porcine epidemic diarrhea virus (PEDV) strain, JS2008, was isolated from stool samples of a piglet with acute diarrhea on a vaccinated farm in eastern China. We sequenced and analyzed the complete genome of strain JS2008, which will help increase our understanding of the molecular characteristics of the epidemic PEDV in China.
Genome announcements. 01/2013; 1(2).
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Libin Wen,
Kongwang He,
Bin Li,
Xiaomin Wang,
Rong Li Guo,
Zhengyu Yu,
Aihua Mao,
Yanxiu Ni,
Xuehan Zhang,
Junming Zhou,
Lixin Lv,
Jieyuan Jiang
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ABSTRACT: Here, we present the first report of a novel rearranged porcine circovirus type 2 (PCV2) strain named BIV, isolated from both in vitro and in vivo sources. The complete circular genome of BIV is 896 nucleotides in length. The data will help us to update current knowledge of the replication of PCV2 viruses in cell culture and of their molecular evolution, as well as their diagnosis.
Journal of Virology 12/2012; 86(23):13120. · 5.40 Impact Factor
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Libin Wen,
Kongwang He,
Yanxiu Ni,
Xuehan Zhang,
Bin Li,
Xiaomin Wang,
Rong Li Guo,
Zhengyu Yu,
Aihua Mao,
Junming Zhou,
Lixin Lv,
Jieyuan Jiang
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ABSTRACT: We report here for the first time the genome sequence of a rearranged porcine circovirus type 2 (PCV2) strain, CH-IVT1, isolated from PCV2-infected PK-15 cells. The complete circular genome of the CH-IVT1 is 605 nucleotides (nt) in length. The finding will help us to understand the molecular evolution of PCV2 and the relationship between PCV2 and PCV-associated diseases.
Journal of Virology 08/2012; 86(16):8911. · 5.40 Impact Factor
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Libin Wen,
Kongwang He,
Yanxiu Ni,
Xuehan Zhang,
Bin Li,
Xiaomin Wang,
Rong Li Guo,
Zhengyu Yu,
Aihua Mao,
Junming Zhou,
Lixin Lv,
Jieyuan Jiang
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ABSTRACT: We first report here the genome sequences of 4 rearranged porcine circovirus type 2 strains, JSTZ, ZJQDH1, ZJQDH2, and JSHM, isolated from porcine sera in China. The complete circular genomes of these isolates are 578, 483, 574, and 772 nucleotides in length, respectively. They are predicted to be defective interfering particles of porcine circovirus type 2. The findings will help us to understand molecular evolution of porcine circovirus type 2 and the relationship between porcine circovirus type 2 and diseases.
Journal of Virology 05/2012; 86(10):5963. · 5.40 Impact Factor
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Bin Li,
Junjie Ma,
Yanling Liu, Libin Wen,
Zhengyu Yu,
Yanxiu Ni,
Xuehan Zhang,
Junming Zhou,
Rongli Guo,
Xiaomin Wang,
Zhixin Feng,
Yiyi Hu,
Lixin Lv,
Kongwang He
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ABSTRACT: Porcine circovirus type 2 (PCV2) is the etiologic agent of porcine circovirus-associated disease. Here, we first report the complete genome sequence of PCV2 strain JSTZ, which was isolated from piglet stool samples and is highly prevalent in China. It will help in understanding the epidemiology and molecular characteristics of PCV2.
Journal of Virology 04/2012; 86(8):4716. · 5.40 Impact Factor
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ABSTRACT: Partetravirus is a novel defined genus of animal parvoviruses. Here, we first report the genome sequence of porcine partetravirus strain JSNJ62, which is highly prevalent in mainland China. It will help in understanding the epidemiology and molecular characteristics of the porcine partetravirus.
Journal of Virology 02/2012; 86(3):1899. · 5.40 Impact Factor
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Libin Wen,
Kongwang He,
Qi Xiao,
Zhengyu Yu,
Aihua Mao,
Yanxiu Ni,
Xuehan Zhang,
Bin Li,
Xiaomin Wang,
Rongli Guo,
Junming Zhou,
Lixin Lv,
Jieyuan Jiang
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ABSTRACT: A novel porcine pathogen tentatively named P1, which was obtained from the sera of the pigs exhibiting clinical signs of postweaning multisystemic wasting syndrome (PMWS) experimentally caused the classical clinic signs and pathologic lesions of the disease in pigs by direct in vivo injection with P1 DNA plasmids. Twenty colostrum-fed (CF) pigs that were free of PCV2 and P1 at 1 month of age were randomly designated equally to two groups. Group 1 pigs were each injected with 400 µg of the cloned P1 plasmid DNA into the superficial inguinal lymph nodes and Group 2 were injected with same amount of the empty pSK vector DNA and served as controls. Viremias were positively detected in 8 of 10 P1 infected pigs from 14-21 days post-inoculation (dpi). The 8 infected animals showed pallor of skin and diarrhea. Gross lesions in the pigs euthanized on 35 dpi were similarly characterized by encephalemia, haemorrhage of the bladder mucosa, haemorrhage of the superficial inguinal lymph nodes, lung atrophy and haemorrhage. Histopathological lesions were arteriectasis and telangiectasia of the cavitas subarachnoidealis, interstitial pneumonia, mild atrophy of the cardiac muscle cells, histiocytic hyperplasia of the follicles in the tonsils, and haemorrhage of the inguinal lymph nodes. P1 DNA and antigens were confirmed by PCR and immunohistochemistry in the tissues and organs of the infected pigs, including the pancreas, bladders, testicles/ovaries, brains, lungs and liver. There were no obvious clinical signs and pathological lesions in the control pigs. This study demonstrated that P1 infection is one of the important pathologic agents on pig farms.
PLoS ONE 01/2012; 7(8):e41565. · 4.09 Impact Factor
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LiBin Wen,
KongWang He,
ZhengYu Yu,
AiHua Mao,
YanXiu Ni,
XueHan Zhang,
RongLi Guo,
Bin Li,
XiaoMin Wang,
JunMing Zhou,
LiXin Lv
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ABSTRACT: We report here the genome sequence of a porcine circovirus-like agent. The sequenced genome of this porcine circovirus-like agent is composed of a 648-nucleotide circular DNA that includes three predicted protein-coding genes, which means the agent should be a novel member of the family Circoviridae.
Journal of Virology 01/2012; 86(1):639. · 5.40 Impact Factor
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Bin Li,
Junjie Ma,
Shaobo Xiao,
Liurong Fang,
Songlin Zeng, Libin Wen,
Xuehan Zhang,
Yanxiu Ni,
Rongli Guo,
Zhengyu Yu,
Junming Zhou,
Aihua Mao,
Lixin Lv,
Xiaoming Wang,
Kongwang He
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ABSTRACT: Porcine bocavirus 5 is a novel porcine bocavirus species found in a pig with clinical diarrhea from a farm in China. Here, we report the complete genome sequence of strain PBoV5/JS677, which will help toward understanding the molecular and evolutionary characteristics of the porcine bocavirus.
Journal of Virology 01/2012; 86(2):1286-7. · 5.40 Impact Factor
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Libin Wen,
Kongwang He,
Hanchun Yang,
Zhengyu Yu,
Aihua Mao,
Shulin Zhong,
Yanxiu Ni,
Xuehan Zhang,
Bin Li,
Xiaomin Wang,
Junming Zhou,
Rongli Guo,
Lixin Lv,
Jieyuan Jiang
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ABSTRACT: Recently, we identified a novel porcine circovirus type 2-like agent P1 isolate from swine. The present study represents the first survey of P1 prevalence in swine herds from Jiangsu, China, by using PCR targeting the complete genome of P1. Prevalences of 50% and 19% were found among 6 herds and 248 animals, respectively. The results indicate a high prevalence of P1 in China pig populations.
Virology Journal 12/2011; 8:543. · 2.34 Impact Factor
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Bin Li,
Shaobo Xiao,
Junjie Ma,
Yanling Liu,
Li Mao, Libin Wen,
Aihua Mao,
Xuehan Zhang,
Yanxiu Ni,
Rongli Guo,
Junming Zhou,
Zhengyu Yu,
Lixin Lv,
Xiaomin Wang,
Liurong Fang,
Huanchun Chen,
Kongwang He
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ABSTRACT: The recently discovered porcine boca-like virus (Pbo-likeV) is a member of the Parvoviridae family, genus Bocavirus, and it is potentially associated with swine disease. Several studies have associated Pbo-likeV with postweaning multisystemic wasting syndrome in pigs, but the full spectrum of clinical disease and the epidemiology of Pbo-likeV infection remain unclear. The availability of rapid and reliable molecular diagnostics would aid future studies of this novel virus. Thus, we developed a sensitive and specific TaqMan-based real-time PCR assay to target the Pbo-likeV NP1 gene. The assay reproducibly detected 20 copies of a recombinant DNA plasmid containing the NP1 gene, with a dynamic range of six orders of magnitude (10(2)-10(7) copies). The assay did not cross-react with other animal viruses. Clinical evaluation found that Pbo-likeV was present in Chinese swine herds at a frequency of 44.2% (114/258). Higher infection rates were found in diseased pigs (56.1%, 101/180) compared with healthy pigs (16.7%, 13/78) (P < 0.05). Our assay for the diagnosis and quantification of Pbo-likeV was highly sensitive and specific, and should provide a reliable real-time tool for epidemiological and pathogenetic study of Pbo-likeV infection.
Virology Journal 01/2011; 8:357. · 2.34 Impact Factor
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ABSTRACT: A novel agent (hence termed as P2) was isolated from pig sera in China, which contained covalently bound circular genomic DNAs of 993 nucleotides. Sequence analyses indicated that the agent was closely related to the porcine circovirus (PCV). The molecular clone of P2 was constructed subsequently and used for the following studies. Intracytoplasmic inclusions and intranuclear inclusions were only found in PK-15 cells transfected with the tandem dimer of P2 molecular DNA clone. Intracytoplasmic inclusions were round or irregular in shape and 0.1-0.4 microm in diameter, and intranuclear inclusions were electronically denser than intracytoplasmic inclusions and had two general shapes: round/small (0.1 microm in diameter) and hexagonal/large (0.5-1.4 microm in diameter). The inclusions were not membranously bound. The cells transfected with the tandem dimer of P2 molecular DNA clone were tested positive for P2 DNA at passages 5. The P2 antigen could be detected in both transfected and passaged PK-15 cells. This is the first report regarding the complete nucleotide sequence of a small DNA genome in a circovirus-like infectious agent in vitro.
Science in China Series C Life Sciences 06/2008; 51(5):453-8. · 1.61 Impact Factor
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ABSTRACT: Genotypes of porcine circovirus type 2 (PCV2) in clinical tissue specimens collected from pigs of different region in China between 2001 and 2003 were analyzed by PCR and restriction fragment length polymorphism (RFLP) analysis of PCV2 genomic DNA encompassing the complete ORF2. The results showed that nine different genotypes (A-I) were identified and designated CHN-2A, CHN-2B, CHN-2C, CHN-2D, CHN-2E, CHN-2F, CHN-2G, CHN-2H and CHN-2I, respectively. Amongst the genotypes, 0.6% were CHN-2A (1/173), CHN-2B (1/173) and CHN-2C (1/173) RFLP profile; 2.3% were CHN-2F (4/173) and CHN-2G (4/173); 5.8% were CHN-2D (10/173); 8.6% were CHN-2E (15/173); 18.5% were CHN-2I (32/173) and 60.7% were CHN-2H (105/173). Therefore, our results suggest that CHN-2H is the dominant genotype of PCV2 prevailing in China. Sequence analysis revealed that ORF2 genes of different genotypic PCV2 exhibited the variation extent of 90.5-99.5% and 88-100% in nucleotide and amino acid respectively. The deduced amino acid sequences alignment of the capsid protein encoded by ORF2 of PCV2 presented that three major regions with greater heterogeneity existed at residues 57-90, 121-136 and 180-191 among nine genotypic PCV2. It was concluded that there exist variation in ORF2 genes of different genotypic PCV2 prevailing in China.
Veterinary Microbiology 10/2005; 110(1-2):141-6. · 3.33 Impact Factor
