ABSTRACT: To investigate the effect of alcohol and/or IL-13 on the expression of collagen type I alpha 1 chain gene (COL1A1, COL3A1) mRNA and collagen production of human lung fibroblast(HFL-1).
HFL-1 was cultured and real time RT-PCR was used to determine the expression of IL-13 receptor (IL-13Rα1, IL-13Rα2, IL-4Rα) mRNA, COL1A1 mRNA and COL3A1 mRNA. ELISA was used to determine the production of collagen type I.
Low level of Alcohol (25, 50, 100, 200 mmol/L) greatly up-regulated the expression of IL-13Rα1 mRNA and IL-4Rα mRNA of HFL-1, but IL-13Rα2 mRNA expression was decreased. low level of alcohol (25, 50, 100, 200 mmol/L) had no effect on the expression of COL1A1 and COL3A1. IL-13 (10, 20, 50 μg/L) stimulated the expression of COL1A1 and COL3A1 in a dose-dependent manner. The expression of COL1A1 and COL3A1 induced by combination of alcohol (200 mmol/L) and IL-13(10 μg/L, 20 μg/L, 50 μg/L) was significantly higher than IL-13(10, 20, 50 μg/L) alone. Combination of alcohol (200 mmol/L) and IL-13(10, 20, 50 μg/L) could significantly increase collagen typeI production than IL-13(10, 20, 50 μg/L) alone.
Low level of alcohol has no effect on the mRNA expression of COL1A1 and COL3A1 of HFL-1cells, but has great effect on the mRNA expression of IL-13Rα1, IL-13Rα2 and IL-4Rα. Combination of alcohol and IL-13 significantly up-regulated the collagen production and expression of COL1A1 and COL3A1 of HFL-1 cells.
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology 04/2011; 27(4):395-8.
ABSTRACT: To observe the inhibitory effects of tyrosine kinases inhibitor A77 1726 on collagen generation induced by IL-13 in fibroblasts.
The inhibition rate of fibroblast proliferation with different concentration of A77 1726 was observed by MTT method. The fibroblasts were divided into the experimental group (A77 1726 50 micromol/L and IL-13 100 microg/L) and the control group (IL-13 100 microg/L). After 24, 48 and 72 hours, the inhibitory effects of A77 1726 on collagen secretion of fibroblasts investigated by hydroxyproline release assay. The inhibitory effects of A77 1726 on collagen type I alpha1 gene mRNA expression in fibroblasts were examinated by RT-PCR. The influence of A77 1726 on collagen type I synthesis in fibroblasts was analyzed by Western blot.
The proliferation of fibroblasts was inhibited by A77 1726. Total collagen generation was down-regulated after 48 h and 72 h stimulation of A77 1726 (P<0.05). The expression level of collagen type I alpha1 gene mRNA was obviously lower in the experimental group than in the control group after 48 h and 72 h stimulation of A77 1726 (P<0.05). Collagen type I production of fibroblasts treated with A77 1726 for 48 and 72 hours was decreased obviously in the experimental group than in the control group (P<0.05).
Tyrosine kinases inhibitor A77 1726 has an inhibitory effect on collagen protein synthesis in fibroblasts induced by IL-13.
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology 01/2009; 25(1):16-9.
ABSTRACT: The insecticidal strain 173 was isolated from marine source and its activity was explored by the bioassay of brine shrimp and Helicoverpa armigera. Based on morphological, physiological and molecular properties, the insecticidal strain 173 was identified as Streptomycetes avermitilis, which is the best insecticidal microorganism found in the terrestrial environment. The taxonomy of the strain 173, insecticidal spectrum and properties of the corresponding insecticidal antibiotics are reported.
Journal of Environmental Sciences 02/2005; 17(1):123-5. · 1.66 Impact Factor