Kuan Li

Publications (2)6.16 Total impact

• Source

  Available from: ricedata.cn

  Article: Involvement of OsSPX1 in phosphate homeostasis in rice.

  Chuang Wang, Shan Ying, Hongjie Huang, Kuan Li, Ping Wu, Huixia Shou
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  ABSTRACT: Arabidopsis thaliana SPX (SYG/PHO81/XPR1) domain genes have recently been shown to be involved in the phosphate (Pi) signaling pathway. We show here that a rice (Oryza sativa) SPX gene, OsSPX1, is specifically induced by Pi starvation in roots. Suppression of OsSPX1 by RNA interference resulted in severe signs of toxicity caused by the over-accumulation of Pi, similar to that found in OsPHR2 (phosphate starvation response transcription factor 2) overexpressors and pho2 (phosphate-responsive mutant 2). Quantitative RT-PCR showed that expression of OsSPX1 was strongly induced in OsPHR2 overexpression and pho2 mutant plants, indicating that OsSPX1 occurs downstream of OsPHR2 and PHO2. The expression of 10 genes associated with the phosphate-starvation signal pathways was analyzed. Expression of OsPT2 (phosphate transporter 2) and OsPT8 was significantly induced in OsSPX1-RNAi (OsSPX1-Ri) plants, suggesting that over-accumulation of Pi in OsSPX1-Ri plants results from an increase in Pi transport. In contrast, overexpression of OsSPX1 suppressed the induction of expression by Pi starvation of all 10 phosphate starvation-induced genes tested: IPS1 (induced by phosphate starvation 1), IPS2, OsPAP10 (purple acid phosphatase 10), OsSQD2 (sulfoquinovosyldiacylglycerol 2), miR399d and miR399j (microRNA 399), OsPT2, OsPT3, OsPT6 and OsPT8. This suggests that OsSPX1 acts via a negative feedback loop to optimize growth under phosphate-limited conditions.
  The Plant Journal 12/2008; 57(5):895-904. · 6.16 Impact Factor
• Article: Comparing Genetic Characteristics of Retrotransposon TOS17 During Different Tissue Culture Processes in the Rice Cultivars Nipponbare and Shishoubaimao

  Kuan LI, Chuang WANG, Shan YING, Hui-xia SHOU
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  ABSTRACT: As a retrotransposon, TOS17 was a useful tool for rice genetic and functional genomic research. To ascertain the feasibility of constructing a TOS17 insertion mutation library in the rice cultivar Shishoubaimao, the genetic and expression characteristics of TOS17 were analyzed. We made solid and suspension tissue cultures and confirmed the copy numbers of TOS17 at different time points in both tissue culture processes by real-time quantitative PCR (RT-qPCR). Three primary copies of TOS17 were detected in naturally grown Shishoubaimao. TOS17 was activated by tissue culture, and the copy numbers of TOS17 increased along with a prolonged tissue culture time in both the Nipponbare and the Shishoubaimao cultivars. Therefore, Shishoubaimao is a potential candidate for constructing a TOS17 insertion mutant library. Compared with Nipponbare, TOS17 was more active in Shishoubaimao during tissue culture. Higher copy numbers of TOS17 were obtained with the suspension tissue culture process than with the solid tissue culture process over the same time courses. We concluded that 3–4 months of suspension tissue culture time is suitable for constructing a TOS17 insertion mutant library in Shishoubaimao.
  Agricultural Sciences in China.