Kisan M Kodam

Publications (11)35.52 Total impact

• Article: Simultaneous reduction of Cr(VI) and oxidation of As(III) by Bacillus firmus TE7 isolated from tannery effluent.

  Sachin P Bachate, Vinod S Nandre, Niraj S Ghatpande, Kisan M Kodam
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  ABSTRACT: Hexavalent chromium [Cr(VI)] and arsenite [As(III)] are the most toxic forms of chromium and arsenic respectively, and reduction of Cr(VI) to Cr(III) and oxidation of As(III) to As(V) has great environmental implications as they affect toxicity and mobility of these toxic species. Bacillus firmus strain TE7, resistant to chromium and arsenic was isolated from tannery effluent. The strain exhibited ability to reduce Cr(VI) and oxidize As(III). It reduced 100mgL(-1) Cr(VI) within 60h in nutrient broth and oxidized 150mgL(-1) As(III) within 10h in minimal medium. It also completely reduced 15mgL(-1) Cr(VI) and oxidized 50mgL(-1) of As(III) simultaneously in minimal medium. To the best of our knowledge, this is the first bacterial strain showing simultaneous reduction of Cr(VI) and oxidation of As(III) and is a potential candidate for bioremediation of environments contaminated with these toxic metal species.
  Chemosphere 11/2012; · 3.21 Impact Factor
• Article: Synthesis and biological evaluation of novel 2,4,6-triazine derivatives as antimicrobial agents.

  Sandip N Gavade, Vijay L Markad, Kisan M Kodam, Murlidhar S Shingare, Dhananjay V Mane
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  ABSTRACT: A series of 2,4,6-trisubstituted [1,3,5]triazines were synthesized and evaluated for their antimicrobial activity against two representative Gram-positive, Gram-negative bacteria and two fungi. Biological data revealed that among all the compounds screened, compounds 3f, 3g, 3h, 3i, 3m, 3o and 3p found to have promising antimicrobial activity against all the selected pathogenic bacteria and fungi. Out of the synthesized compounds seven analogues have shown MIC in the range of 6.25-12.5 μg/mL. These compounds were generally nontoxic and may prove useful as antimicrobial agents.
  Bioorganic & medicinal chemistry letters 06/2012; 22(15):5075-7. · 2.65 Impact Factor
• Article: Effective bioremoval and detoxification of textile dye mixture by Alishewanella sp. KMK6.

  Yogesh M Kolekar, Pallavi D Konde, Vijay L Markad, Sharwari V Kulkarni, Ashvini U Chaudhari, Kisan M Kodam
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  ABSTRACT: Alishewanella sp. strain KMK6 was able to degrade mixture of textile dyes (0.5-2.0 g l(-1)) within 8 h. An initial 28 % reduction in COD was observed immediately after decolorization at static anoxic conditions which on further incubation at shaking conditions reduced by 90 %. Partially purified azoreductase was able to utilize different azo dyes as substrates. The HPLC profile of dye degradation showed formation of metabolic products. Further FTIR analysis showed significant changes in the peaks corresponding to functional groups present in dye mixture and its degradation products. The genotoxicity assessment showed that the dye degradation products were non-toxic compared to dye mixture.
  Applied Microbiology and Biotechnology 03/2012; · 3.42 Impact Factor
• Article: Oxidation of arsenite by two β-proteobacteria isolated from soil.

  Sachin P Bachate, Rashmi M Khapare, Kisan M Kodam
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  ABSTRACT: Two heterotrophic As(III)-oxidizing bacteria, SPB-24 and SPB-31 were isolated from garden soil. Based on 16S rRNA gene sequence analysis, strain SPB-24 was closely related to genus Bordetella, and strain SPB-31 was most closely related to genus Achromobacter. Both strains exhibited high As(III) (15 mM for SPB-24 and 40 mM for SPB-31) and As(V) (>300 mM for both strains) resistance. Both strains oxidized 5 mM As(III) in minimal medium with oxidation rate of 554 and 558 μM h(-1) for SPB-24 and SPB-31, respectively. Washed cells of both strains oxidized As(III) over broad pH and temperature range with optimum pH 6 and temperature 42°C for both strains. The As(III) oxidation kinetic by washed cells showed K (m) and V (max) values of 41.7 μM and 1,166 μM h(-1) for SPB-24, 52 μM and 1,186 μM h(-1) for SPB-31. In the presence of minimal amount of carbon source, the strains showed high As(III) oxidation rate and high specific arsenite oxidase activity. The ability of strains to resist high concentration of arsenic and oxidize As(III) with highest rates reported so far makes them potential candidates for bioremediation of arsenic-contaminated environment.
  Applied Microbiology and Biotechnology 03/2012; 93(5):2135-45. · 3.42 Impact Factor
• Article: Effect of fly ash on biochemical responses and DNA damage in earthworm, Dichogaster curgensis.

  Vijaykumar L Markad, Kisan M Kodam, Vikram S Ghole
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  ABSTRACT: Fly ash is receiving alarming attention due to its hazardous nature, widespread usage, and the manner of disposal; leading to environmental deterioration. We carried out bio-monitoring and risk assessment of fly ash in earthworms as a model system. Dichogaster curgensis were allowed to grow in presence or absence of fly ash (0-40%, w/w) for 1, 7, and 14 d. The biochemical markers viz. catalase (CAT), superoxide dismutase (SOD), glutathione reductase (GR), glutathione peroxidase (GPx), glutathione S-transferase (GST), and malondialdehyde (MDA) level were measured. The comet and neutral red retention assays were performed on earthworm coelomocytes to assess genetic damages and lysosomal membrane stability. The results revealed increased activities of SOD, GPx, GST, and MDA level in a dose-response manner while GR activity was decreased with increasing concentrations of fly ash. No obvious trend was observed in the CAT activity and fly ash concentration. Lysosomal membrane destabilization was noted in the earthworms exposed to 5% and more fly ash concentration in a dose and time dependent manner. The comet assay demonstrated that the fly ash induced DNA damage and DNA-protein crosslinks in earthworm coelomocytes.
  Journal of hazardous materials 02/2012; 215-216:191-8. · 4.14 Impact Factor
• Article: Effective bioremoval and detoxification of textile dye mixture by Alishewanella sp. KMK6

  Yogesh M. Kolekar, Pallavi D. Konde, Vijay L. Markad, Sharwari V. Kulkarni, Ashvini U. Chaudhari, Kisan M. Kodam
  Applied Microbiology and Biotechnology 01/2012; · 3.42 Impact Factor
• Article: Decolorization and biodegradation of azo dye, reactive blue 59 by aerobic granules.

  Yogesh M Kolekar, Harshal N Nemade, Vijay L Markad, Sunil S Adav, Milind S Patole, Kisan M Kodam
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  ABSTRACT: The present study deals with development of aerobic granules from textile wastewater sludge and challenged with different concentration of reactive blue 59 (RB59) to test their dye degradation potential. The granules efficiently degraded reactive blue 59 and also sustained higher dye loading of up to 5.0 g l(-1). The significant induction of enzymes azoreductase and cytochrome P-450 indicated their prominent role in the dye degradation while genotoxicity studies demonstrated that the biotransformed product of the dye as non-toxic. The microbial community of the textile dyes degrading aerobic sludge granules analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), revealed significantly diverse dye degrading microbial community belonging to alpha-, beta-, and gamma-proteobacteria.
  Bioresource technology 11/2011; 104:818-22. · 4.25 Impact Factor
• Article: Decolorization of textile dyes by Alishewanella sp. KMK6.

  Yogesh M Kolekar, Kisan M Kodam
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  ABSTRACT: Alishewanella sp. strain KMK6 was isolated from textile dye-contaminated soil. The strain was able to decolorize and degrade different azo dyes and displayed high dye degradation ability and tolerance. The bacterium could completely degrade 2.5 g l(-1) dye, Reactive Blue 59 within 6 h. The induction in the level of cytochrome P-450 and activities of azoreductase and NADH-dichlorophenolindophenol reductase were observed in the cells after dye decolorization indicating the role of these enzymes. The intermediates of Reactive Blue 59 degradation were identified by high-performance liquid chromatography, gas chromatography and mass spectroscopy, and Fourier transform infrared spectroscopy. The ecotoxicity has been evaluated for dye and its metabolites by 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (a yellow tetrazole) and comet assay, and it revealed that the dye degradation products were nontoxic.
  Applied Microbiology and Biotechnology 11/2011; 95(2):521-9. · 3.42 Impact Factor
• Article: Effect of a novel biphenyl compound, VMNS2e on ob/ob mice.

  Sucheta B Kurundkar, Narsingh Sachan, Kisan M Kodam, Vithal M Kulkarni, Subhash L Bodhankar, Vikram S Ghole
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  ABSTRACT: VMNS2e is a novel biphenyl compound, which in previous studies had showed most favourable interactions with the active site of protein tyrosine phosphatase 1B (PTP1B). The effect of acute and chronic treatment of VMNS2e (30mg/kg) was investigated in ob/ob mice. Plasma glucose was measured after acute administration of VMNS2e (30mg/kg) in both lean and ob/ob mice. In the chronic study, VMNS2e (30mg/kg) was given orally, once daily for 60days. Metformin (300mg/kg) was taken as standard therapy. Body weight, food intake and blood glucose was measured weekly while glycosylated hemoglobin A(1c) (HbA(1c)), insulin, triglyceride, total cholesterol, low density lipoprotein (LDL), fructosamine, non esterified fatty acid and organ weight were estimated after the completion of treatment period. Oral glucose tolerance test was performed on the last day of treatment. Liver and epididymal fat weights were taken. Acute dose of VMNS2e elicited an anti hyperglycemic effect. It reduced blood glucose by 14% (0.5h) and 35.6% (6h). Chronic VMNS2e treatment improved glucose tolerance by 25.3%. It decreased blood glucose levels. Hyperinsulinemia was reduced (19.6%). VMNS2e treatment had no significant effect on body weight and food consumption. VMNS2e treatment exhibited significant reduction (28.2%) in HbA(1c), plasma triglyceride (49%), LDL (24%) and fructosamine (13%) levels. VMNS2e treatment did not alter total cholesterol and non esterified fatty acid levels. Epididymal fat/body weight ratio was reduced (26.3%). VMNS2e exhibited both acute and chronic anti hyperglycemic effect, insulin sensitivity along with improvement in various lipid parameters and glycemic control.
  European journal of pharmacology 10/2010; 650(1):472-8. · 2.59 Impact Factor
• Article: Novel biphenyl compound, VMNS2e, ameliorates streptozotocin-induced diabetic nephropathy in rats.

  Sucheta B Kurundkar, Narsingh Sachan, Kisan M Kodam, Vithal M Kulkarni, Subhash L Bodhankar, Serena D'Souza, Geeta Vanage, Vikram S Ghole
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  ABSTRACT: To study the effect of a new biphenyl synthetic compound showing interactions with the active site of protein tyrosine phosphatase 1B by docking and molecular dynamics, VMNS2e in streptozotocin-induced diabetic nephropathy in rats with various renal function parameters and renal ultrastructure. Streptozotocin (55 mg/kg)-induced diabetic rats were orally treated once daily with VMNS2e (30, 60, and 120 mg/kg) for 8 weeks. The body weight and blood glucose levels of the rats were recorded during the study period. After 8 weeks of treatment creatinine clearance, urinary protein, blood urea nitrogen, urinary albumin excretion rate, and insulin levels were measured. An ultrastructure study of the kidney tissue was performed and the glomerular basement membrane thickness was measured. Eight weeks of VMNS2e treatment significantly reduced the fasting blood glucose level, attenuated elevating blood urea nitrogen levels, and reduced glomerular basement membrane thickness. It is concluded that VMNS2e treatment at 30 and 60 mg/kg, when given for 8 weeks, partly ameliorated early diabetic nephropathy in diabetic rats.
  Journal of Diabetes 09/2010; 2(4):282-9.
• Article: Decolorization and degradation of Disperse Blue 79 and Acid Orange 10, by Bacillus fusiformis KMK5 isolated from the textile dye contaminated soil.

  Yogesh M Kolekar, Shrikant P Pawar, Kachru R Gawai, Pradeep D Lokhande, Yogesh S Shouche, Kisan M Kodam
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  ABSTRACT: The release of azo dyes into the environment is a concern due to coloration of natural waters and due to the toxicity, mutagenicity and carcinogenicity of the dyes and their biotransformation products. The dye degrading bacterial strain KMK 5 was isolated from the textile dyes contaminated soil of Ichalkaranji, Maharashtra, India. It was identified as Bacillus fusiformis based on the biochemical and morphological characterization as well as 16S rDNA sequencing. KMK 5 could tolerate and degrade azo dyes, Disperse Blue 79 (DB79) and Acid Orange 10 (AO10) under anoxic conditions. Complete mineralization of DB79 and AO10 at the concentration of 1.5g/l was observed within 48h. This degradation potential increased the applicability of this microorganism for the dye removal.
  Bioresource Technology 07/2008; 99(18):8999-9003. · 4.98 Impact Factor