Irene Mittermann,
Renate Reininger,
Maya Zimmermann, Katharina Gangl,
Jürgen Reisinger,
Karl J Aichberger,
Elli K Greisenegger,
Verena Niederberger,
Joachim Seipelt,
Barbara Bohle,
Tamara Kopp,
Cezmi A Akdis,
Susanne Spitzauer,
Peter Valent,
Rudolf Valenta
[show abstract]
[hide abstract]
ABSTRACT: Hom s 2, the alpha-chain of the nascent polypeptide-associated complex, is an intracellular autoantigen that has been identified with IgE autoantibodies from atopic dermatitis patients. We investigated the humoral and cellular immune response to purified recombinant Hom s 2 (rHom s 2). rHom s 2 exhibited IgE reactivity comparable to exogenous allergens, but did not induce relevant basophil cell degranulation. The latter may be attributed to the fact that patients recognized single epitopes on Hom s 2 as revealed by IgE epitope mapping with rHom s 2 fragments. In contrast to exogenous allergens, rHom s 2 had the intrinsic ability to induce the release of IFN-gamma in cultured peripheral blood mononuclear cells from atopic as well as non-atopic individuals. IFN-gamma-containing culture supernatants from Hom s 2-stimulated peripheral blood mononuclear cells caused disintegration of respiratory epithelial cell layers and apoptosis of skin keratinocytes, which could be inhibited with a neutralizing anti-IFN-gamma antibody. Our data demonstrate that the Hom s 2 autoantigen can cause IFN-gamma-mediated cell damage.
Journal of Investigative Dermatology 07/2008; 128(6):1451-9. · 6.31 Impact Factor
