[Show abstract][Hide abstract] ABSTRACT: Alterations in the cellular biological responses were examined in a series of LNCaP human prostate tumor cells growing under different conditions. Materials and
LNCaP cells were grown in two-dimensional monolayer cultures, three-dimensional spheroids, or as solid tumors in immune-deprived mice.
As compared with the growth in the monolayers, cell growth in the spheroids was reduced, while VEGF production was increased. Immunohistochemical analysis of the spheroids revealed that cells showing Ki-67 up-regulation were localized in the peripheral layer, and that the central core was necrotic. The gene expression profile in the solid tumor tissue was obviously different from that in the monolayers; however, it was similar to that in the spheroids. The prostate-specific antigen levels in the culture supernatants of spheroids increased with time and decreased with anticancer agent treatment.
Spheroid formation from human prostate tumor cells exhibits tissue-like features.
Anticancer research 01/2007; 27(1A):45-53. · 1.83 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Poly(ADP-ribose) polymerase (PARP 1 ; EC 2. 4. 2. 30) is a constitutively expressed enzyme that mediates the normal cel-lular response to DNA damage and interferes with cell differentiation through transcriptional control. PARP 1-deficient mice have been re-ported to exhibit teeth displasia similar to human odontoma. In the present study, we evaluated the contribution of PARP 1 to odontoma development by immunohistochemical staining of human odontoma sections with an anti-PARP 1 antibody. PARP 1 was expressed in odontoblasts, immature enamel and the epithelial component corresponding to ameloblasts. Interestingly, in odontoma, PARP 1 was ex-pressed in the cytoplasm of the ghost cells, which had no nucleus and showed positive staining for cytokeratins. Moreover, the ghost cells showed positive staining with the M30 CytoDEATH antibody, which recognizes a caspase cleavage product of cytokeratin 18 observed in the process of apoptosis, suggesting that some very early events of apoptosis have occurred in these ghost cells expressing PARP 1.