[Show abstract][Hide abstract] ABSTRACT: Endothelial nitric oxide synthase (eNOS) has an important role in erection, and it also affects aspects of sexual behavior. In this experiment, we determined whether a compound enhancing the activity of eNOS, Impaza, could stimulate any aspect of sexual behavior and increase penis length in rats with a high baseline of sexual activity. For comparison, the PDE5 inhibitor sildenafil was included. Male rats were orally treated with Impaza or sildenafil for 28 days. Impaza (3 ml kg(-1)) was given daily while sildenafil (3 mg kg(-1)) was given twice weekly. Tests for sexual incentive motivation and copulatory behavior were performed just before drug treatment and at days 7, 14 and 28 of treatment. In addition, the length of the protruding penis at mount, intromission and ejaculation was measured. Impaza but not sildenafil increased penis length at mount after 14 and 28 days of treatment. The compounds failed to modify sexual incentive motivation or copulatory behavior. It is suggested that Impaza enhanced intracavernous pressure, as such a pressure increase is the most likely explanation for enhanced penis length at mount. This effect, together with an absence of motivational actions, suggests that Impaza may be the most valuable treatment for erectile dysfunction.International Journal of Impotence Research advance online publication, 14 March 2013; doi:10.1038/ijir.2013.12.
International journal of impotence research 03/2013; 26(1). DOI:10.1038/ijir.2013.12 · 1.76 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: We estimated the efficacy of Brizantin preparation in suppressing nicotine dependence in rats. It was shown that nicotine-dependent rats in the situation of choice between the chamber with smoke or the chamber with food more frequently entered the chamber with tobacco smoke and stayed there longer. The rats that received Brizantin demonstrated significantly fewer visits to the chamber with smoke and spent there less time. Reduced locomotor activity and orientation and exploratory behavior in rats against the background of Brizantin administration also suggest reduced motivation for smoke inhalation. Thus, Brizantin effectively diminished nicotine dependence in rats in the model of nicotine addiction.
Bulletin of Experimental Biology and Medicine 12/2012; 154(3):339-342. DOI:10.1007/s10517-013-1945-3 · 0.36 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: The capacity of a new drug containing ultra-low doses of antibodies to cannabinoid receptor type 1 (Dietressa) to reduce body weight gain in mice on a high-calorie diet was evaluated, possible mechanisms of drug action were analyzed, and its safety (abuse potential in the reaction of self-stimulation) was evaluated. Dietressa was not inferior to sibutramine in reducing body weight gain in mice and exhibited no abuse potential.
Bulletin of Experimental Biology and Medicine 07/2012; 152(3):321-4. DOI:10.1007/s10517-012-1518-x · 0.36 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Antidiabetic activity of Subetta was revealed on the model of streptozotocin-induced diabetes mellitus in rats. Intragastric administration of this preparation in a dose of 5 ml/kg for 50 days reduced blood glucose levels, urine levels of glucose and ketone bodies, restored glucose tolerance in the oral glucose test, improved general condition and increased the survival rate of animals. The effectiveness of the drug was not inferior to that of rosiglitazone (8 mg/kg).
Bulletin of Experimental Biology and Medicine 05/2012; 153(1):54-6. DOI:10.1007/s10517-012-1641-8 · 0.36 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: We compared two modifications of Vogel conflict test and assessed anxyolitic activity of two drugs: diazepam (benzodiazepine anxiolitic) and tenoten (ultra-low doses of antibodies to S-100 protein) in both modifications of the test. It was found that the intensity of anxiolitic effect of the drugs depends on the conditions of Vogel test.
Bulletin of Experimental Biology and Medicine 07/2011; 151(3):336-9. DOI:10.1007/s10517-011-1324-x · 0.36 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Experiments on different models for sexual behavior (seasonal and age-related inhibition of sexual function, animals with initially reduced sexual function) showed that ultra-low doses of anti-NO-synthase antibodies (Impaza) stimulate sexual motivation and copulative behavior in rats. The effects of the drug on different aspects of sexual behavior depend on the chosen model.
Bulletin of Experimental Biology and Medicine 02/2011; 150(4):500-3. DOI:10.1007/s10517-011-1178-2 · 0.36 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Therapeutic and preventive treatment of mice intranasally infected with a lethal dose of A/Aichi/2/68 (H3N2) influenza virus with anaferon (pediatric formulation) demonstrated an antiviral effect of the drug (increased percent of survivors and prolonged lifespan).
Bulletin of Experimental Biology and Medicine 12/2009; 146(6):763-5. DOI:10.1007/s10517-009-0378-5 · 0.36 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Course administration of ultralow doses of antibodies to S-100 protein restores cognitive functions and neurological status, improves emotional state, and reduces anxiety in animals with modeled Alzheimer disease based on cholinergic system deficiency caused by subchronic treatment with scopolamine.
Bulletin of Experimental Biology and Medicine 09/2009; 148(3):533-5. DOI:10.1007/s10517-010-0757-y · 0.36 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Kardos, a preparation containing ultralow doses of antibodies to C-terminal fragment of type 1 receptor of angiotensin II, intragastrically administered to SHR rats with hereditary hypertension for 28 days reduced blood pressure by 14.8%. Kardos was not inferior to losartan and, in contrast to the latter reduced HR by 9.4%.
Bulletin of Experimental Biology and Medicine 09/2009; 148(3):520-3. DOI:10.1007/s10517-010-0753-2 · 0.36 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: The antiamnestic effects of tenoten (pediatric formulation) was demonstrated on the model of scopolamine-induced amnesia of passive avoidance reflex and the nootropic effect of this preparation was demonstrated on the model of incomplete conditioning and in rat pups with experimental attention deficit syndrome. The efficiency of the preparation was comparable to that of piracetam and phenibut and even surpassed it by some parameters.
Bulletin of Experimental Biology and Medicine 09/2009; 148(3):524-6. DOI:10.1007/s10517-010-0754-1 · 0.36 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Experiments on rats demonstrated antiaggressive activity of ultralow doses of antibodies to S-100 protein in tests of motivated and unmotivated aggression. The effect of ultralow doses of antibodies to S-100 protein in single and course treatment was not inferior to that of benzodiazepine anxiolytic diazepam.
Bulletin of Experimental Biology and Medicine 09/2009; 148(3):527-9. DOI:10.1007/s10517-010-0755-0 · 0.36 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Ultralow doses of antibodies to S-100 protein increased rat survival, reduced neurological deficit, eliminated myorelaxation, and improved movement coordination and cognitive functions in rats with experimental hemorrhagic stroke; the efficiency of the preparation was not inferior to that of nimodipine. In contrast to nimodipine, ultralow doses of antibodies to S-100 protein exhibited pronounced anxiolytic properties.
Bulletin of Experimental Biology and Medicine 09/2009; 148(3):530-2. DOI:10.1007/s10517-010-0756-z · 0.36 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Introduction. Earlier studies have shown that sildenafil may modify some aspects of male rat sexual behavior and sexual incentive motivation. Stimulation of endothelial nitric oxide synthase (eNOS) has also been reported to affect sexual motivation in old rats.
Aim. To determine the effects of sildenafil and a compound stimulating eNOS on copulatory behavior and sexual incentive motivation in young adult Fisher 344 and Wistar male rats.
Methods. The rats were selected for a low intromission ratio, and then treated with Impaza (stimulator of eNOS), sildenafil, or Impaza + sildenafil for 28 days. Tests for copulatory behavior and sexual incentive motivation were performed before the beginning of treatment and at days 7, 14, and 28 of treatment.
Main Outcome Measures. Standard parameters of copulatory behavior and sexual incentive motivation. Measurements of penis length at mount, intromission, and ejaculation.
Results. The Fisher 344 rats displayed a higher level of sexual incentive motivation than the Wistar rats, while the copulatory behavior was similar in both strains. Impaza and sildenafil enhanced the sexual incentive motivation after 28 days of treatment in the Wistar rats, but failed to do so in the Fisher 344 rats. The copulatory behavior was unaffected in the Wistar strain, while the Fisher 344 males had an enhanced intromission ratio after treatment with Impaza and sildenafil for 28 days.
Conclusions. The nitric oxide-guanylyl cyclase pathway seems to be of importance for sexual incentive motivation in animals with a modest baseline level. The different drug effects in the Wistar and Fisher 344 rats can be attributed to baseline differences. The importance of eNOS for sexual functions should not be overlooked. Chu X, Zhavbert ES, Dugina JL, Kheyfets IA, Sergeeva SA, Epstein OI, and Ågmo A. Sildenafil and a compound stimulating endothelial NO synthase modify sexual incentive motivation and copulatory behavior in male Wistar and Fisher 344 rats. J Sex Med 2008;5:2085–2099.
Journal of Sexual Medicine 08/2008; 5(9):2085 - 2099. DOI:10.1111/j.1743-6109.2008.00937.x · 3.15 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Antibodies to endothelial NO synthase in ultralow doses exhibited anxiolytic and antidepressant effects and their efficiency after single and course treatment is not inferior to that of amitriptyline and diazepam. The psychotropic activity of ultralow-dose antibodies to endothelial NO-synthase is presumably one of important mechanisms of their efficiency in the treatment of erectile dysfunction.
Bulletin of Experimental Biology and Medicine 07/2008; 145(6):735-7. DOI:10.1007/s10517-008-0193-4 · 0.36 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Parallel treatment with haloperidol and ultralow-dose haloperidol significantly increased the psychotropic neuroleptic effect of traditional doses of the drug under conditions of preliminary of simultaneous administration, which attests to a bipathic effect of this preparation. Combination of ultralow and therapeutic doses of haloperidol significantly reduced its cataleptogenic side effect.
Bulletin of Experimental Biology and Medicine 06/2008; 145(5):620-2. DOI:10.1007/s10517-008-0156-9 · 0.36 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: S100 proteins are small calcium-binding proteins interacting with numerous intra- and extra cellular targets involved in diverse physiological functions. In particular, S100 proteins may be involved in the regulation of anxiety-related behaviour. In the present study, the effects of affinity-purified antibodies to S100 proteins administered orally at ultra-low doses were evaluated in pre-clinical tests for anxiolytic-like activity in the adult rat. In the Vogel conflict test in the rat, antibodies to S100 proteins increased punished drinking (anti-conflict effect) at 5 and 7.5 mL kg(-1), but not at 2.5 or 10 mL kg(-1). Antibodies to S100 proteins increased the percentage of entries into the open arms of an elevated plus-maze at 10 mL kg(-1), but not at lower doses. Taken together, these results indicate the presence of anxiolytic-like activity for antibodies to S100 proteins over the dose range 5-10 mL kg(-1) in the adult rat.
Journal of Pharmacy and Pharmacology 04/2008; 60(3):309-16. DOI:10.1211/jpp.60.3.0005 · 2.26 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Afala (ultralow-dose antibodies to prostate-specific antigen) injected for 60 days to rats with hormone-induced prostatitis caused by sulpiride prevented the development of prostatic hyperplasia and reduced the severity of histological changes. The effect of Afala was superior to that of the reference drug (Serenoa repens extract).
Bulletin of Experimental Biology and Medicine 12/2007; 144(5):699-701. DOI:10.1007/s10517-007-0409-z · 0.36 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Phenazepam in ultralow doses significantly potentiated anxiolytic and anticonvulsant effects of therapeutic doses of the same drug both after preliminary or simultaneous administration, which attests to bipathic action of phenazepam. The combination of ultralow and therapeutic doses of phenazepam prevented the development of its specific myorelaxant and sedative side effects.
Bulletin of Experimental Biology and Medicine 10/2007; 144(4):536-8. DOI:10.1007/s10517-007-0371-9 · 0.36 Impact Factor