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ABSTRACT: The aim of the study was to follow the development of microchimerism after allogeneic vascularized bone marrow transplantation (VBMT) versus conventional bone marrow transplantation (BMT). In one group, a VBMT model consisted of donor Brown Norway rat hind limb heterotopic transplanted on recipient Lewis rats. An intravenous infusion of donor bone marrow cells in suspension equivalent to that grafted in the vascularized femur limb was administered intravenously to recipient rats in the second group. Cellular microchimerism was investigated in recipients of VBMT versus BMT. Donor-derived cells could be detected in VBMT recipients at 30 and 60 days but not in recipients of intravenous suspension of BMC. VBMT provides a theoretical alternative to conventional cellular bone marrow transplantation by addressing crucial clinical problems such as failure of engraftment or graft-versus-host disease.
Transplantation Proceedings 11/2011; 43(9):3549-51. · 1.00 Impact Factor
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Plastic & Reconstructive Surgery 07/2011; 128:622. · 3.38 Impact Factor
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D G Zamfirescu,
E Owen,
I Lascar,
M Molitor, I Zegrea,
M Popescu,
G A Bishop,
C A Lauer,
M Simionescu,
M Climov,
M Lanzetta
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ABSTRACT: Previous studies have demonstrated that composite tissue transplants such as limbs reject more slowly than skin transplants. This has led to the hypothesis that a simultaneous skin graft may act as an effective marker of limb rejection. The aim of this study was to test the predictive value of a sentinel skin graft as a marker of rejection, using a hind limb transplantation model in rats. Lewis rat recipients received hind limb transplants alone from a Brown Norway donor (control; n = 15) or combined with a full-thickness 15 cm(2) sentinel skin graft (n = 45). All animals received drug therapy (tacrolimus, mycophenolate mofetil, and prednisone) for 6 weeks; then, treatment was ceased entirely. Rejection of the skin graft and limb skin was assessed both by visual and histologic grading systems. Detectable visual rejection (grade 1) was observed earlier in the sentinel skin graft than in the limb skin (P < .0005); the clearest visual rejection (grade 2) appeared earlier in the sentinel skin graft (P < .005). The average histologic grade for early rejection of the skin graft was 1.46 and 1.08 for the limb skin (P < .05). These findings confirmed a visual and histologic delay in the rejection of limb skin compared with a distant sentinel skin graft. Skin grafts transplanted simultaneously with hind limbs may be a useful marker of early rejection.
Transplantation Proceedings 04/2009; 41(2):503-8. · 1.00 Impact Factor
