Haruhiko Madarame

Publications (6)12.21 Total impact

• Article: Haemostatic and inflammatory responses to blood flow-restricted exercise in patients with ischaemic heart disease: a pilot study.

  Haruhiko Madarame, Miwa Kurano, Kazuya Fukumura, Taira Fukuda, Toshiaki Nakajima
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  ABSTRACT: Low-intensity resistance exercise can effectively induce muscle hypertrophy and increases in strength when combined with moderate blood flow restriction (BFR). As this type of exercise does not require lifting heavy weights, it might be a feasible method of cardiac rehabilitation, in which resistance exercise has been recommended to be included. Although previous studies with healthy subjects showed relative safety of BFR exercise, we cannot exclude the possibility of unfavourable effects in patients with cardiovascular disease. We therefore aimed to investigate haemostatic and inflammatory responses to BFR exercise in patients with ischaemic heart disease (IHD). Nine stable patients with IHD who were not taking anticoagulant drugs performed four sets of knee extension exercise at an intensity of 20% one-repetition maximum (1RM) either with or without BFR. Blood samples were taken before, immediately after and 1 h after the exercise session and analysed for noradrenaline, D-dimer, fibrinogen/fibrin degradation products (FDP) and high-sensitive C-reactive protein (hsCRP). Plasma noradrenaline concentration increased after the exercise, and the increase was significantly larger after the exercise with BFR than without BFR. On the other hand, increases in concentrations of plasma D-dimer and serum hsCRP were independent of the condition. However, increases in D-dimer and hsCRP were no longer observed after plasma volume correction, suggesting that hemoconcentration was responsible for these increases. Plasma FDP concentration did not change after the exercise. These results suggest that applying BFR during low-intensity resistance exercise does not affect exercise-induced haemostatic and inflammatory responses in stable IHD patients.
  Clinical Physiology and Functional Imaging 01/2013; 33(1):11-7. · 1.33 Impact Factor
• Article: Blood flow-restricted training does not improve jump performance in untrained young men.

  Haruhiko Madarame, E Ochi, Y Tomioka, K Nakazato, N Ishii
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  ABSTRACT: The purpose of this study was to investigate the effect of blood flow-restricted training (BFRT) on jump performance in relation to changes in muscle strength. Seventeen untrained young men were assigned into either BFRT or normal training (NORT) groups and performed low-intensity [30-40% of one-repetition maximum (1RM)] resistance exercise (horizontal squat, 3-4 sets × 15-30 repetitions) twice a week for 10 weeks. The BFRT performed the exercise with their proximal thighs compressed by air-pressure cuffs for the purpose of blood flow restriction. Squat 1RM, muscle cross-sectional area (CSA) of quadriceps femoris, and countermovement jump (CMJ) height were measured before and after the 10-wk training period. Squat 1RM increased greater in BFRT than in NORT (19.3% vs. 9.7%, P < 0.01). Although the CSA increase was independent of groups, it tended to be larger in BFRT than in NORT (8.3% vs. 2.9%, P = 0.094). On the other hand, CMJ height did not change after the training (P = 0.51). In conclusion, the present study showed that BFRT induced muscle hypertrophy and strength increase, whereas it did not increase CMJ height in previously untrained young men. It is suggested that BFRT is ineffective in improving jump performance.
  Acta Physiologica Hungarica 12/2011; 98(4):465-71. · 0.82 Impact Factor
• Article: Increase in serum growth hormone induced by electrical stimulation of muscle combined with blood flow restriction.

  Yoshitaka Inagaki, Haruhiko Madarame, Mitsuo Neya, Naokata Ishii
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  ABSTRACT: The purpose of this study was to investigate the role of muscle metaboreflex on exercise-induced growth hormone (GH) secretion. In order to accumulate metabolites within exercised muscle with minimized central motor activity, electromyostimulation (EMS) was performed combined with blood flow restriction (BFR). Seven men performed one-legged isometric knee extension evoked by EMS (frequency, 20 Hz; pulse duration, 400 μs; on-off ratio, 3-1 s). Just before the exercise, proximal portion of either a stimulated thigh (ST) or a non-stimulated thigh (NT) was compressed at 150 mmHg with an air-pressure cuff for the purpose of BFR. The compression was kept throughout the exercise session, and was released 2 min after the end of the exercise. Two exercise sessions (ST(BFR), BFR for ST; NT(BFR), BFR for NT) were separated by 1 week. ST(BFR) was aimed to accumulate metabolites within exercised muscle, whereas NT(BFR) was aimed to match mechanical stress with ST(BFR) without accumulating metabolites. Blood samples for hormonal measurements were taken from the antecubital vein before and after the exercise. Blood lactate increased immediately after the exercise in the NT(BFR), whereas it increased after the cuff deflation in the ST(BFR), suggesting that locally produced metabolites were retained and accumulated within the exercised muscle in the ST(BFR). Although serum cortisol and plasma noradrenaline increased in a similar manner in two conditions, serum immunoreactive GH (irGH) increased only in the ST(BFR). These results suggest that muscle metaboreflex plays an important role in the exercise-induced GH secretion, at least in terms of irGH secretion.
  Arbeitsphysiologie 03/2011; 111(11):2715-21. · 2.15 Impact Factor
• Article: Pentraxin3 and high-sensitive C-reactive protein are independent inflammatory markers released during high-intensity exercise.

  Toshiaki Nakajima, Miwa Kurano, Takaaki Hasegawa, Haruhito Takano, Haruko Iida, Tomohiro Yasuda, Taira Fukuda, Haruhiko Madarame, Kansei Uno, Kentaro Meguro, Taro Shiga, Mina Sagara, Taiji Nagata, Koji Maemura, Yasunobu Hirata, Tatsuya Yamasoba, Ryozo Nagai
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  ABSTRACT: High-intensity exercise shares similarities with acute phase responses of inflammatory diseases. We investigated the influences of acute exercise on inflammatory markers, plasma pentraxin3 (PTX3) and serum high-sensitive C-reactive protein (CRP) (hsCRP). Nine healthy male subjects (41 ± 3 years old) participated. Each subject performed three types of exercise; ergometer exercise at 70% workload of anaerobic threshold (AT) for 30 min (70% AT exercise), peak ergometer exercise (peak EX, 20 watt increase/min until fatigue) and resistance exercises of 70% 1 RM (70% RE) until exhaustion. We measured plasma PTX3, serum hsCRP, lactate, noradrenaline (NOR), white blood cells (WBC), interleukin-6 (IL-6) and myeloperoxidase (MPO), a marker of neutrophil degranulation. The effects of exercise on intracellular PTX3 and MPO in neutrophils were also investigated, by using flow cytometry analysis. Circulating PTX3 and hsCRP significantly increased immediately after 70% RE and peak EX, while they did not increase after 70% AT exercise. The exercise-induced fold increase in PTX3 and hsCRP relative to the resting level was positively correlated with the changes in WBC, NOR, lactate and MPO. The exercise-induced fold increase in IL-6 was positively correlated with that in NOR, but not with that in PTX3 and hsCRP. Neutrophils isolated immediately after 70% RE, but not 70% AT exercise, exhibited lower mean fluorescence for PTX3 and MPO than those from pre-exercise blood. These results provide the evidence that high-intensity exercises significantly increase circulatory PTX3 as well as hsCRP. The release from peripheral neutrophils is suggested to be involved in the exercise-induced plasma PTX3 increase.
  Arbeitsphysiologie 11/2010; 110(5):905-13. · 2.15 Impact Factor
• Article: Effects of low-intensity resistance exercise with blood flow restriction on coagulation system in healthy subjects.

  Haruhiko Madarame, Miwa Kurano, Haruhito Takano, Haruko Iida, Yoshiaki Sato, Hiroshi Ohshima, Takashi Abe, Naokata Ishii, Toshihiro Morita, Toshiaki Nakajima
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  ABSTRACT: Recent studies have demonstrated that even a low-intensity resistance exercise can effectively induce muscle hypertrophy and strength increase when combined with moderate blood flow restriction (BFR) into the exercising muscle. Although serious side effects of low-intensity resistance exercise with BFR have not been reported, a concern of thrombosis has been suggested, because this type of exercise is performed with restricted venous blood flow and pooling of blood in extremities. Thus, the purpose of this study was to investigate the effects of low-intensity resistance exercise with BFR on coagulation system in healthy subjects. Ten healthy men (25.1 +/- 2.8 year) performed four sets of leg press exercises with and without BFR (150-160 mmHg) at an intensity of 30% of one-repetition maximum (1RM). In each exercise session, one set with 30 repetitions was followed by three sets with 15 repetitions. Blood samples were taken before, and 10 min, 1, 4 and 24 h after the exercise. Prothrombin fragment 1 + 2 (PTF) and thrombin-antithrombin III complex (TAT) were measured as markers of thrombin generation, whereas D-dimer and fibrin degradation product (FDP) were measured as markers of intravascular clot formation. Changes in plasma volume (PV) were calculated from haemoglobin and hematocrit values. PV reduction was significantly greater after the exercise with BFR than without (P<0.05). However, neither markers of thrombin generation nor intravascular clot formation increased after the exercises. These results suggest that low-intensity resistance exercise with BFR does not activate coagulation system in healthy subjects.
  Clinical Physiology and Functional Imaging 02/2010; 30(3):210-3. · 1.33 Impact Factor
• Article: Cross-transfer effects of resistance training with blood flow restriction.

  Haruhiko Madarame, Mitsuo Neya, Eisuke Ochi, Koichi Nakazato, Yoshiaki Sato, Naokata Ishii
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  ABSTRACT: This study investigated whether muscle hypertrophy-promoting effects are cross-transferred in resistance training with blood flow restriction, which has been shown to evoke strong endocrine activation. Fifteen untrained men were randomly assigned into the occlusive training group (OCC, N = 8) and the normal training group (NOR, N = 7). Both groups performed the same unilateral arm exercise (arm curl) at 50% of one-repetition maximum (1RM) without occlusion (three sets, 10 repetitions). Either the dominant or nondominant arm was randomly chosen to be trained (OCC-T, NOR-T) or to serve as a control (OCC-C, NOR-C). After the arm exercise, OCC performed leg exercise with blood flow restriction (30% of 1RM, three sets, 15-30 repetitions), whereas NOR performed the same leg exercise without occlusion. The training session was performed twice a week for 10 wk. In a separate set of experiments, acute changes in blood hormone concentrations were measured after the same leg exercises with (N = 5) and without (N = 5) occlusion. Cross-sectional area (CSA) and isometric torque of elbow flexor muscles increased significantly in OCC-T, whereas no significant changes were observed in OCC-C, NOR-T, and NOR-C. CSA and isometric torque of thigh muscles increased significantly in OCC, whereas no significant changes were observed in NOR. Noradrenaline concentration showed a significantly larger increase after leg exercise with occlusion than after exercises without occlusion, though growth hormone and testosterone concentrations did not show significant differences between these two types of exercises. The results indicate that low-intensity resistance training increases muscular size and strength when combined with resistance exercise with blood flow restriction for other muscle groups. It was suggested that any circulating factor(s) was involved in this remote effect of exercise on muscular size.
  Medicine &amp Science in Sports &amp Exercise 03/2008; 40(2):258-63. · 4.43 Impact Factor