Li Zhang,
Zhang Li,
Hua Yu,
Yu Hua,
Wen Ming Li,
Li Wen Ming,
Man Chun Cheung,
Cheung Man Chun,
Yuan Ping Pang,
Pang Yuan Ping,
Ge Lin,
Lin Ge,
Yi Tao Wang,
Wang Yi Tao,
Zhong Zuo,
Zuo Zhong, Yi Fan Han,
Han Yi Fan
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ABSTRACT: The current study aims to develop a specific and sensitive LC-MS/MS method for determination of bis(7)-tacrine (B7T) in rat plasma. A 100 microL plasma sample was extracted with ethyl acetate. B7T and the internal standard (IS), pimozide, in the samples were then analyzed with LC-MS/MS in positive electrospray ionization condition. Chromatographic separation of B7T and IS was achieved in a C(18) reversed-phase HPLC column (150 x 2.1 mm i.d.) by isocratic elution with a mobile phase consisting of 0.05% formic acid in water and acetonitrile (1:1, v/v) at a flow rate of 0.35 mL/min. Multiple-reaction monitoring (MRM) mode was employed to measure the ion transitions: m/z 247 to 197 for B7T and m/z 462 to m/z 328 for IS, respectively. The method was linear over the studied ranges of 100-5000 and 10-100 ng/mL. The intra-day and inter-day variations of the analysis were less than 6.8% with standard errors less than 9.0%. The detection limit of B7T in rat plasma was 1 ng/mL. The developed method was successfully applied to the pharmacokinetic study of B7T after intravenous administration of 1 mg/kg B7T and further proved to be readily utilized for determination of B7T in rat plasma samples.
Biomedical Chromatography 05/2008; 22(4):414-20. · 1.97 Impact Factor
