Gui Li Song

Publications (2)16.38 Total impact

• Article: Developmental changes in functional expression and beta-adrenergic regulation of I(f) in the heart of mouse embryo.

  Gui Li Song, Ming Tang, Chang Jin Liu, Hong Yan Luo, Hua Min Liang, Xin Wu Hu, Jiao Ya Xi, Lin Lin Gao, Bernd Fleischmann, Jürgen Hescheler
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  ABSTRACT: The hyperpolarization-activated current (I(f)) plays an important role in determining the spontaneous rate of cardiac pacemaker cells. The automatic rhythmicity also exists in working cells of embryonic heart, therefore we studied developmental changes in functional expression and beta-adrenergic regulation of I(f) in embryonic mouse heart. The expression of I(f) is high in early developmental stage (EDS) (10.5 d after coitus) ventricular myocytes, low in intermediate developmental stage (IDS) (13.5 d) atrial or ventricular myocytes and even lower in late developmental stage (LDS) (16.5 d) atrial or ventricular myocytes, indicating that these cells of the EDS embryonic heart have some properties of pacemaker cells. Beta-adrenergic agonist isoproterenol (ISO) stimulates I(f) in LDS but not in EDS cardiomyocytes, indicating that the beta-adrenergic regulation of I(f) is not mature in EDS embryonic heart. But forskolin (a direct activator of adenylate cyclase) and 8-Br-cAMP (a membrane-permeable analogue of cAMP) increase the amplitude of I(f) in EDS cells, indicating that adenylate cyclase and cAMP function fairly well at early stage of development. Furthermore, the results demonstrate that I(f) is modulated by phosphorylation via cAMP dependent PKA both in EDS and LDS cells.
  Cell Research 01/2003; 12(5-6):385-94. · 8.19 Impact Factor
• Article: Developmental changes in functional expression and -adrenergic regulation of If in the heart of mouse embryo

  Gui Li SONG, Ming TANG, Chang Jin LIU, Hong Yan LUO, Hua Min LIANG, Xin Wu HU, Jiaoya XI, Lin Lin GAO, Bernd FLEISCHMANN, J|[uuml]|rgen HESCHELER
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  ABSTRACT: The hyperpolarization-activated current (If) plays an important role in determining the spontaneous rate of cardiac pacemaker cells. The automatic rhythmicity also exists in working cells of embryonic heart, therefore we studied developmental changes in functional expression and -adrenergic regulation of If in embryonic mouse heart. The expression of If is high in early developmental stage (EDS) (10.5 d after coitus) ventricular myocytes, low in intermediate developmental stage (IDS) (13.5 d) atrial or ventricular myocytes and even lower in late developmental stage (LDS) (16.5 d) atrial or ventricular myocytes, indicating that these cells of the EDS embryonic heart have some properties of pacemaker cells. -adrenergic agonist isoproterenol (ISO) stimulates If in LDS but not in EDS cardiomyocytes, indicating that the b-adrenergic regulation of If is not mature in EDS embryonic heart. But forskolin (a direct activator of adenylate cyclase) and 8-Br-cAMP (a membrane-permeable analogue of cAMP) increase the amplitude of If in EDS cells, indicating that adenylate cyclase and cAMP function fairly well at early stage of development. Furthermore, the results demonstrate that If is modulated by phosphorylation via cAMP dependent PKA both in EDS and LDS cells.Keywords: embryonic cardiomyocyte, development, If, -adrenergic
  Cell Research 11/2002; 12(5):385-394. · 8.19 Impact Factor