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ABSTRACT: The aim of this study is to evaluate impact of a new polymeric contraceptive SMA-Fe<sub>3</sub>O<sub>4</sub>-Cu-DMSO called Smart RISUG (acronym for Smart Reversible Inhibition of Sperm under Guidance), a colloidal suspension of styrene maleic anhydride co-polymer (SMA), nano-micro iron oxide (Fe<sub>3</sub>O<sub>4</sub>) and copper powder (Cu) (one milligram) dissolved in dimethylsulphoxide (DMSO) (1:30); on Albino rat`s sperm solution (one milliliter). Experiments to assess the morphology and viability of control as well as treated sperm cells were performed by using microscopic techniques like High Resolution Transmission Electron Microscopy (HRTEM), Field Emission Scanning Electron Microscopy (FESEM), Atomic Force Microscopy (AFM), Scanning Electron Microscopy (SEM)-X ray microanalysis, phase contrast microscopy and Fluorescent Activated Cell Sorting (FACS). Treated cells indicate uniform adhesion of Smart RISUG particles to the sperm cell membrane, topological alteration, decrease in the cell count, reduced cell motility and viability, increased sperm abnormality and complete cell inactivation in about 72 h. This study suggests use of smart RISUG as a potential non-invasively reversible male/female contraceptive in future.
International Journal of Pharmacology. 01/2009;