Geórgia D M Marques

Publications (3)4.43 Total impact

• Article: Expression of TLR-4 and -2 in peripheral mononuclear cells in renal transplant patients with TLR-4 gene polymorphism.

  Eliana Nogueira, Reinaldo Salomao, Milena Karina Colló Brunialti, Kikumi S Ozaki, Geórgia D M Marques, Marcos A Cenedeze, Niels Olsen Saraiva Câmara, Alvaro Pacheco-Silva
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  ABSTRACT: TLR-4 has also been identified as a receptor for endogenous alarmins, which are increased post transplantation. TLR-4 has also been associated with a polymorphism that could impact graft outcome. To assess the expression of TLR-4 in kidney transplant patients carrying or not a polymorphism. TLR-4 polymorphism (A299G/T399I) was studied in 200 renal transplant patients. Healthy volunteers were also enrolled as control group. The polymorphism analysis was performed using restriction enzymes technique (RFLP). Functionality of TLR-4 polymorphism was assessed in samples from controls by quantification of TNF-α after LPS stimulus. TLR-4 and -2 expressions were also analyzed by flow cytometry. TLR-4 polymorphism was present in 8.5% of renal transplant patients. This polymorphism was associated with impairment in TNF-α secretion. In general, in renal transplant patients, TLR-4 expression in monocytes and in neutrophils was lower than in health volunteers. TLR-2 and TLR-4 expressions in healthy volunteers with A299G/T399I TLR-4 polymorphism was higher than in wild-type genotype healthy volunteers (p<0.01 and p<0.05, respectively), and also higher than A299G/T399I TLR-4 polymorphism renal transplant patients (p<0.05). TLR-2 expression on neutrophils in wild-type genotype renal transplant patients was higher compared to wild-type genotype healthy volunteers, and was also higher in relation to A299G/T399I kidney transplanted patients (p<0.01). Stable renal transplant patients with TLR-4 polymorphism have a lower expression of TLR-4 and TLR-2 receptors in peripheral mononuclear cells, which ultimately indicate a less responsiveness for alarmins.
  International immunopharmacology 10/2010; 10(12):1481-5. · 2.21 Impact Factor
• Article: Toll-like receptors-related genes in kidney transplant patients with chronic allograft nephropathy and acute rejection.

  Eliana Nogueira, Viviane Campos Ponciano, Erika L Naka, Geórgia D M Marques, Marcos A Cenedeze, Niels Olsen Saraiva Câmara, Alvaro Pacheco-Silva
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  ABSTRACT: Toll-like receptors (TLR) comprehend an emerging family of receptors that recognize pathogen-associated molecular patterns and promote the activation of leukocytes. Surgical trauma and ischemia-reperfusion injury are likely to provide exposure to endogenous ligands for TLR in virtually all kidney transplant recipients. Macroarray (GEArray OHS-018.2 Series-Superarray) analyses of 128 genes involved in TLR signaling pathway were performed in nephrectomy samples of patients with chronic allograft nephropathy (CAN) and acute rejection (AR, vascular and non vascular). The analysis of each membrane was performed by GEArray Expression Analysis Suite 2.0. Macroarray profile identified a gene expression signature that could discriminate CAN and AR. Three genes were significantly expressed between CAN and vascular AR: Pellino 2; IL 8 and UBE2V1. In relation to vascular and non-vascular AR, there were only two genes with statistical significance: IL-6 and IRAK-3. Vascular and non-vascular AR and CAN showed different expression of a few genes in TLR pathway. The analysis of nephrectomy showed that activation of TLR pathway is present in AR and CAN.
  International immunopharmacology 01/2009; 9(6):673-6. · 2.21 Impact Factor
• Article: Toll-like receptors-related genes in kidney transplant patients with chronic allograft nephropathy and acute rejection

  Eliana Nogueira, Viviane Campos Ponciano, Érika L. Naka, Geórgia D.M. Marques, Marcos A. Cenedeze, Niels Olsen Saraiva Câmara, Alvaro Pacheco-Silva
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  ABSTRACT: IntroductionToll-like receptors (TLR) comprehend an emerging family of receptors that recognize pathogen-associated molecular patterns and promote the activation of leukocytes. Surgical trauma and ischemia–reperfusion injury are likely to provide exposure to endogenous ligands for TLR in virtually all kidney transplant recipients.MethodsMacroarray (GEArray OHS-018.2 Series-Superarray) analyses of 128 genes involved in TLR signaling pathway were performed in nephrectomy samples of patients with chronic allograft nephropathy (CAN) and acute rejection (AR, vascular and non vascular). The analysis of each membrane was performed by GEArray Expression Analysis Suite 2.0.ResultsMacroarray profile identified a gene expression signature that could discriminate CAN and AR. Three genes were significantly expressed between CAN and vascular AR: Pellino 2; IL 8 and UBE2V1. In relation to vascular and non-vascular AR, there were only two genes with statistical significance: IL-6 and IRAK-3.ConclusionVascular and non-vascular AR and CAN showed different expression of a few genes in TLR pathway. The analysis of nephrectomy showed that activation of TLR pathway is present in AR and CAN.
  International Immunopharmacology.