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Lucindo Quintans-Júnior,
Ricardo Fagundes da Rocha,
Fernanda Freitas Caregnato,
José Claudio Fonseca Moreira, Francilene Amaral da Silva,
Adriano Antunes de Souza Araújo,
João Paulo Almeida dos Santos,
Mônica Santos Melo,
Damião Pergentino de Sousa,
Leonardo Rigoldi Bonjardim,
Daniel Pens Gelain
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ABSTRACT: Citronellal (CT) is a monoterpenoid and the major constituent of the mixture of terpenoids that give the citronella oil its lemon scent. Citronella oil is widely used around the world for various purposes and is mainly obtained from plants of the Cymbopogon genus, which are known as "lemongrass." Considering these plants have been used worldwide for various medicinal purposes, the interest of researchers to understand the biological activities of monoterpenoids related to the Cymbopogon genus has been increasing. In the present work, we investigated the antinociceptive action and the redox properties of CT. Our results indicate that intraperitoneal injection of CT was effective in reducing nociceptive face-rubbing behavior in both phases of the formalin test, which was also naloxone-sensitive. CT also evoked antinociceptive response in the capsaicin and glutamate tests. The total radical-trapping antioxidant parameter and total antioxidant reactivity assays indicate that CT at doses of 0.1 and 1 mg/mL exerts a significant antioxidant activity, which is probably related to its ability to scavenge superoxide and nitric oxide, but not H(2)O(2) or hydroxyl radicals, as evaluated separately by specific in vitro tests. These results show for the first time the antinociceptive potential of CT and indicate that the antioxidant properties of this compound may rely on its mechanism of biological actions because CT-containing natural products are used to treat various diseases related to oxidative stress and reactive species.
Journal of medicinal food 06/2011; 14(6):630-9. · 1.39 Impact Factor
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Marcelia Garcez Dória Melo,
João Paulo Almeida dos Santos,
Mairim Russo Serafini,
Fernanda Freitas Caregnato,
Matheus Augusto de Bittencourt Pasquali,
Thallita Kelly Rabelo,
Ricardo Fagundes da Rocha,
Lucindo Quintans,
Adriano Antunes de Souza Araújo, Francilene Amaral da Silva,
José Cláudio Fonseca Moreira,
Daniel Pens Gelain
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ABSTRACT: Atranorin (ATR) is a lichenic secondary metabolite with potential uses in pharmacology. Antinociceptive and antiinflammatory actions have been reported, and the use of atranorin-enriched lichen extracts in folk medicine is widespread. Nonetheless, very few data on ATR biological actions are available. Here, we evaluated free radical scavenging activities and antioxidant potential of ATR using various in vitro assays for scavenging activity against hydroxyl radicals, hydrogen peroxide, superoxide radicals, and nitric oxide. The total reactive antioxidant potential (TRAP) and total antioxidant reactivity (TAR) indexes and in vitro lipoperoxidation were also evaluated. Besides, we determined the cytoprotective effect of ATR on H(2)O(2)-challenged SH-SY5Y cells by the MTT assay. ATR exerts differential effects towards reactive species production, enhancing hydrogen peroxide and nitric oxide production and acting as a superoxide scavenger; no activity toward hydroxyl radical production/scavenging was observed. Besides, TRAP/TAR analysis indicated that atranorin acts as a general antioxidant, although it demonstrated to enhance peroxyl radical-induced lipoperoxidation in vitro. ATR was not cytotoxic, and also protected SH-SY5Y cells against H(2)O(2)-induced cell viability impairment. Our results suggest that ATR has a relevant redox-active action, acting as a pro-oxidant or antioxidant agent depending on the radical. Also, it will exert cytoprotective effects on cells under oxidative stress induced by H(2)O(2).
Toxicology in Vitro 03/2011; 25(2):462-8. · 2.78 Impact Factor
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Evandro Gomes da Silva,
Guilherme Antonio Behr,
Alfeu Zanotto-Filho,
Rodrigo Lorenzi,
Matheus Augusto de Bittencourt Pasquali,
Luis Gustavo Ravazolo,
Celso Luis Bordignon, Francilene Amaral da Silva,
Ana Lúcia Aboy,
Valquiria Linck Bassani,
Amélia Teresinha Henriques,
Flávio Henrique Reginatto,
Felipe Dal-Pizzol,
José Cláudio Fonseca Moreira
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ABSTRACT: Aqueous and hydro-ethanolic extracts of Bauhinia microstachya leaves (AEBM and HEBM) were investigated for their phenolic content and phytochemical profile (by spectrophotometry and HPLC), and for their antioxidant activities and free radical scavenging potential in different in vitro systems (TRAP, TEAC, TBARS, nitric oxide, superoxide and hydroxyl radical). HEBM presented a 27.4% higher content of phenolics when compared to AEBM and a distinct phytochemical profile was observed. Our work suggests that both extracts have potent antioxidant activities and that their antioxidant capacity and efficiency vary according to the radical-generating system. In general, HEBM was more effective than AEBM in avoiding ROS-generating damage and in scavenging the various radicals formed. Nevertheless, when results were normalized to total phenolic content, a different profile of antioxidant activities and free radical scavenging potential was observed, particularly against oxidative lipid damage and superoxide radical. B. microstachya extracts may be considered an interesting source of natural antioxidants as well as other phenolic-rich plants.
Biological & Pharmaceutical Bulletin 09/2007; 30(8):1488-96. · 1.66 Impact Factor
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Journal of Liquid Chromatography & Related Technologies 01/2007; 30(20):3119-3131. · 0.71 Impact Factor
