Feng-Rui WU

Publications (2)2.02 Total impact

• Source

  Available from: swu.edu.cn

  Article: Duplication and distinct expression patterns of two thrombospondin-1 isoforms in teleost fishes.

  Feng-Rui Wu, Lin-Yan Zhou, Yoshitaka Nagahama, De-Shou Wang
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  ABSTRACT: Two types of thrombospondin-1 (named TSP-1a and TSP-1b) were cloned from two species of teleosts, the Nile tilapia and medaka. Phylogenetic analysis of these TSP-1 sequences, together with those available from other vertebrates further demonstrated that two types of TSP-1 exist only in teleosts, extending the finding in fugu and tetraodon to two additional fish species. The expression of both genes was examined using tilapia at various developmental stages. Tilapia TSP-1a and TSP-1b were each expressed in a wide range of tissues examined. The early expression of TSP-1b in both XX and XY gonads from 5dah (day after hatching) onwards suggested an important role in the formation of gonads, while the expression of TSP-1a only in ovaries during later stages of development (from 120dah onwards) may suggest that TSP-1a is involved in oogenesis. During the 14-day spawning cycle, the expression of both types of TSP-1 exhibited distinct peaks at day 5 (peak of vitellogenesis) and day 12 (oocyte maturation). In situ hybridization analyses revealed differential expression, with TSP-1a occurring in granulosa cells and TSP-1b in theca cells. Furthermore, both TSP-1a and -1b were expressed in skeletal tissues but with clear temporal and spatial differences. In contrast, only TSP-1b was found in the myosepta. The positive signals of both TSP-1a and TSP-1b were also detected in the heart and spleen, and TSP-1a in brain and intestine by both RT-PCR and in situ hybridization.
  Gene Expression Patterns 07/2009; 9(6):436-43. · 2.02 Impact Factor
• Article: Molecular cloning and expression of two isoforms of Vasa gene in Southern catfish Silurus meridionalis

  Chong-Jiang HU, Feng-Rui WU, Zhi-Hao LIU, Bao-Feng HUANG, Yao-Guang ZHANG, De-Shou Wang
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  ABSTRACT: Two isoforms of Vasa cDNA, derived from the 5′alternative splicing of the same gene, were isolated and characterized in Southern catfish, Silurus meridionalis, by reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) methods. Analysis of the nucleotide sequences revealed that the full length cDNA of Southern catfish Vasa (scVasa) comprises 2525 base pairs (bp) with an open reading frame (ORF) of 1989 bp, encoding 662 amino acids, while that of Vasa short form (scVasa-s) comprises 2438 bp with an ORF of 1926 bp, encoding 641 amino acids. Except the difference in 5′-untranslated region, scVasa-s also lacks a part of the 5′-ORF region found in the scVasa. Both of the two deduced amino acid sequences contain all the eight conserved motifs characteristic to the DEAD-box protein family and four arginine-glycine-glycine motifs unique to Vasa proteins. scVasa showed the highest similarity to Vasa homolog of giebel carp (73.3%). Tissue distribution analysis by RT-PCR revealed that these two isoforms were exclusively expressed in the gonads of both sexes. In adult fish, scVasa was found to be mainly expressed in the primary oocytes at phase Ⅰ and Ⅱ in the ovary while in the spermatogonia and primary spermatocytes in the testis by in situ hybridization. Semi-quantitative RT-PCR analysis showed that the expressions of both scVasa isoforms were much higher in ovarian recrudescent stage (mainly with phase Ⅱ oocytes) than in vitellogenic stage (mainly with phase Ⅲ and Ⅳ oocytes) [Acta Zoologica Sinica 54（6）：1051–1060, 2008].
  Acta Zoologica Sinica. 01/2008;