São Paulo State University, San Paulo, São Paulo, Brazil

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Publications (3)4.17 Total impact

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    ABSTRACT: Milk is an important nutritional source to man and water buffalo raw milk is used to produce mozzarella cheese. Products from unpasteurized milk have been associated with certain infectious diseases and can carry pathogenic mycobacteria. Nontuberculous mycobacteria (NTM) are emerging pathogens causing opportunistic infections in humans and animals. The objectives of this study were to demonstrate the presence of mycobacteria in water buffaloes' milk and to determine their role as possible sources of NTM infections. In this study, raw milk samples from dairy water buffaloes (Bubalus bubalis) (N = 23) were decontaminated by Petroff method and inoculated on to Löwenstein-Jensen and Stonebrink medium. After confirming positive colonies for acid fast bacilli (AFB) by Ziehl-Neelsen technique, the isolated mycobacteria were identified by PCR-Restriction Enzyme Analysis (PRA) and mycolic acids analysis by thin-layer chromatography (TLC). Mycobacterium simiae (2 isolates), Mycobacterium kansasii (2 isolates), Mycobacterium flavescens (2 isolates), Mycobacterium gordonae (3 isolates) and Mycobacterium lentiflavum (1 isolate) were identified by these techniques. The isolation of opportunistic pathogens such as M. kansasii, M. simiae and M. lentiflavum from raw milk represent a risk for the consumers of mozzarella cheese made by this milk.
    Food Microbiology 10/2009; 26(6):658-61. · 3.37 Impact Factor
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    ABSTRACT: O leite é importante como fonte nutricional ao homem, bem como possível via de transmissão de micobactérias, principalmente de Mycobacterium bovis. Neste estudo padronizou-se uma técnica de PCR eficaz para detectar, diretamente no leite, o M. bovis. Para tal, leite tipo A foi inoculado artificialmente com número conhecido de M. bovis AN5, e diluições sucessivas foram submetidas a dois protocolos de PCR para avaliar o limite de detecção de M. bovis. Em um dos protocolos foi empregado o par de primers INS1 e INS2 para detecção do Mycobacterium spp e no outro, o JB21 e JB22 específico para M. bovis. As diluições foram também semeadas no meio de Stonebrink e estes incubados por até 90 dias a 37°C, para determinar o número de bacilos viáveis inoculados no leite. Pelos resultados de PCR, o protocolo empregando o par de primer INS1 e INS2 foi menos sensível detectando bacilos até a diluição 10-3 (800 UFC/mL). O par JB-21 e JB-22 detectou M. bovis até a diluição 10-4 (80 UFC/ mL), sendo portanto mais sensível. Este método pode servir como ferramenta diagnóstica de M. bovis em amostras de leite.
    Alimentos e Nutrição. 01/2008;
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    ABSTRACT: Plants have contributed in a significant way to the treatment of most of the diseases. Considering the therapeutic importance of the medicinal plants, the immunological activity of the methanolic and ethanolic extract of Davilla elliptica was evaluated. In every stage of the immune response macrophages are involved and they can release many compounds such as hydrogen peroxide (H2O2), nitric oxide (NO) and tumor necrosis factor-a (TNF-a). Macrophages stimulation was evaluated by the determination of H2O2, NO and TNF-a in peritoneal macrophages cultures of mice in the presence of the D. elliptica extracts. IC50 was determined by MTT assay. The phytochemical study showed flavonoids derived from quercetin and myricetin and other compounds. The production of H2O2 was not very expressive in both extracts, but they presented a significant effect on NO production. The two extracts induced TNF-a production, although the methanolic liberated almost five times more TNF-a than the ethanolic one. A relationship among the synthesis of NO and TNF-a was observed. The increase of NO production is related with the induction of proinflammatory cytokines like TNF-a. Analyzing the results, it is suggested that methanolic and ethanolic extract of D. elliptica can modulate macrophage activation.
    Revista Brasileira de Farmacognosia 03/2005; 15(1):44-50. · 0.80 Impact Factor