Eugenio Jose Garcia

University of São Paulo, San Paulo, São Paulo, Brazil

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Publications (12)9.27 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Purpose: To evaluate the effect of different test parameters on the resin-dentin microshear bond strength (μSBS). Materials and Methods: A 1.5-mm-thick dentin disk was prepared in each of 140 human molars. The disks were divided into five groups to test the following variables: time of adhesive light polymerization (n = 20), storage time (n = 40), bonding area (n = 40), Tygon tube removal (n = 20), and time of composite placement (n = 20). The adhesives were applied and each specimen was subjected to μSBS testing. All fractured specimens were observed with SEM. The data from each experiment were subjected to two-way ANOVA and Tukey's test (α = 0.05). Results: The storage time, bonding area, and Tygon tube removal did not influence the μSBS. Higher μSBS values were found when empty Tygon tubes were filled after positioning and when the adhesive was light polymerized before Tygon tube placement (p < 0.05). Conclusions: Differences in test parameters affected the μSBS of adhesives, especially the time of adhesive light polymerization and composite placement.
    The journal of adhesive dentistry 06/2014; · 0.91 Impact Factor
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    ABSTRACT: This study aims to evaluate the effect of different application modes on the immediate and 6-month resin-dentin bond strength (μTBS) and nanoleakage in the hybrid and adhesive layers. Three 1-step self-etch adhesives (One Coat 7.0 (OC), Clearfil S(3) Bond (CS), and FuturaBond NR (FB)) were applied on a flat superficial dentin surface of 30 human molars under manual mode or sonic vibration at a frequency of 170 Hz (SV). Composite build-ups were constructed incrementally; specimens were sectioned to obtain resin-dentin sticks with cross-sectional area of 0.8 mm(2) and tested in tension (0.5 mm/min) immediately (IM) or after 6 months (6M) of water storage. Two bonded sticks, from each tooth at each storage time, were immersed in a silver nitrate solution, photo-developed, and analyzed under scanning electron microscopy. The amount of nanoleakage was measured using the ImageTool 3.0 software. Data, from each adhesive, were analyzed by two-way ANOVA and Tukey's test (α = 0.05). OC and CS showed higher μTBS and lower nanoleakage in the IM period when applied with SV groups. For FB, no significant difference was observed between the two modes of application. All materials showed lower nanoleakage in the SV groups. Higher nanoleakage was observed after 6M for OC and FB. The sonic application mode at an oscillating frequency of 170 Hz can improve the resin-dentin μTBS, reduce the nanoleakage, and retard the degradation of the resin-dentin μTBS of Clearfil S(3) Bond and One Coat 7.0 adhesives. Sonic application mode at an oscillating frequency of 170 Hz can be helpful and easy to use in dental practice to guaranty long-lasting restorations.
    Clinical Oral Investigations 06/2013; · 2.20 Impact Factor
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    ABSTRACT: This in vitro study evaluated the influence of two devices for application of shear load in microshear tests on bond strength and fracture pattern of primary enamel and dentin. Eighty primary molars were selected and flat enamel (40 teeth sectioned mesio-distally) and dentin (40 teeth sectioned transversally) surfaces were obtained. Both surfaces were polished to standardize the smear layer. Two-step etch-and-rinse adhesive systems (Adper Single Bond and XP Bond) were used. Polyethylene tubes was placed over the bonded surfaces and filled with composite resin. The microshear testing was performed after storage in water (24 h/37 °C) using two devices for application of microshear loads: a notched rod (Bisco Shear Bond Tester) or a knife edge (Kratos Industrial Equipment). Failure modes were evaluated using a stereomicroscope. Bond strength data were subjected to ANOVA and chi-square test to compare the failure mode distributions (α=0.05). No significant differences were observed between the groups for dentin and enamel bond strength or fracture patterns (p>0.05). The predominant failure mode was adhesive/mixed. In conclusion, the devices for application of shear loads did not influence the bond strength values, regardless of adhesive system and substrate.
    Brazilian dental journal 02/2013; 24(6):605-9.
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    ABSTRACT: Objectives: To evaluate the effect of application time on the resin-dentin bond strength (µTBS) and etching pattern of adhesive systems applied on sclerotic dentine. Material and Methods: A total of forty-two bovine incisors had their roots removed. The 1-step self-etch GO (SDI), the 2-step self-etch Adper SE Bond (3MESPE) and the 35% phosphoric acid (3MESPE) from the 2-step etch-and-rinse Adper Single Bond 2 (3MESPE) were applied on the bovine incisal surfaces according to the manufacturer's instructions or duplicating the recommended conditioning time. After adhesive application, thirty teeth were restored with composite resin, stored for 24 h in distilled water at 37°C, and sectioned into resin-dentin bonded sticks (0.8 mm 2 ) and tested according to the µTBS at 0.5 mm/min. The etching pattern of the remaining twelve teeth (n=4 for each material) was examined under scanning electron microscopy. Each tooth was divided into a buccal-to-lingual direction into three thirds, and each third randomly assigned to the groups: control (no treatment), according to the manufacturers' instructions and duplicating the recommended application time. The µTBS and the relative percentage of the tubule area opening were evaluated by two-way repeated measures ANOVA and Tukey's tests (α=0.05). Results: The duplication of the conditioning time favored only the GO adhesive (p<0.05). Both application methods significantly increased the tubule area opening (p<0.05) compared to the controls. Conclusions: The efficacy of duplicating the conditioning time was only effective for the 1-step self-etch adhesive system tested.
    Journal of applied oral science: revista FOB 01/2013; 21(2). · 0.39 Impact Factor
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    ABSTRACT: Objectives This study aimed to compare the micro-tensile bond strength of methacrylate resin systems to a silorane-based restorative system on dentin after 24 hours and six months water storage. Material and Methods The restorative systems Adper Single Bond 2/Filtek Z350 (ASB), Clearfil SE Bond/Z350 (CF), Adper SE Plus/Z350 (ASEP) and P90 Adhesive System/Filtek P90 (P90) were applied on flat dentin surfaces of 20 third molars (n=5). The restored teeth were sectioned perpendicularly to the bonding interface to obtain sticks (0.8 mm2) to be tested after 24 hours (24 h) and 6 months (6 m) of water storage, in a universal testing machine at 0.5 mm/min. The data was analyzed via two-way Analysis of Variance/Bonferroni post hoc tests at 5% global significance. Results Overall outcomes did not indicate a statistical difference for the resin systems (p=0.26) nor time (p=0.62). No interaction between material × time was detected (p=0.28). Mean standard-deviation in MPa at 24 h and 6 m were: ASB 31.38 (4.53) and 30.06 (1.95), CF 34.26 (3.47) and 32.75 (4.18), ASEP 29.54 (4.14) and 33.47 (2.47), P90 30.27 (2.03) and 31.34 (2.19). Conclusions The silorane-based system showed a similar performance to methacrylate-based materials on dentin. All systems were stable in terms of bond strength up to 6 month of water storage.
    Journal of applied oral science: revista FOB 01/2013; 21(1):80-4. · 0.39 Impact Factor
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    ABSTRACT: To assess the influence of ozone gas and ozonated water application to prepared cavity and bonded interfaces on the resin/dentin bond strength of two-step etch-and-rinse adhesive systems (Adper Single Bond 2 [SB2] and XP-Bond [XP]). Sixty extracted human third molars were sectioned perpendicularly to their long axes to expose flat occlusal dentin surfaces. In experiment 1, dentin was treated with ozone before the bonding procedure, while in experiment 2, ozone was applied to resin/dentin bonded interfaces. In experiment 1, dentin surfaces were treated either with ozone gas (2100 ppm), ozonated water (3.5 ppm), or distilled water for 120 s, and then bonded with SB2 or XP according to manufacturers' instructions. Hybrid composite buildups were incrementally constructed and the teeth were sectioned into resin-dentin sticks (0.8 mm2). In experiment 2, dentin surfaces were first bonded with SB2 or XP, composite buildups were constructed, and bonded sticks obtained. The sticks were treated with ozone as previously described. Bonded sticks were tested under tensile stress at 1 mm/min. Silver nitrate impregnation along the resin/dentin interfaces was also evaluated under SEM. Two-way ANOVA (adhesive and ozone treatment) detected no significant effect for the cross-product interaction and the main factors in the two experiments (p > 0.05), which was confirmed by the photomicrographs. Ozone gas and ozonated water used before the bonding procedure or on resin/dentin bonded interfaces have no deleterious effects on the bond strengths and interfaces.
    The journal of adhesive dentistry 01/2012; 14(4):363-70. · 0.91 Impact Factor
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    ABSTRACT: It is generally not recommended that bonded restoration treatment should be carried out immediately after bleaching treatment. However, the use of antioxidants such as sodium ascorbate can be useful to avoid a waiting period. This article is a brief review about in vitro proposals to overcome low bond strength values to bleached dental surfaces. It shows a one-year follow-up case report of a young female presenting agenesis of maxillary lateral incisors reshaped with direct resin composite immediately after dental bleaching. Teeth were bleached with a combination of in-office and at-home whitening protocols, followed by the application of a 10% sodium ascorbate gel for one hour, to allow the immediate reshaping with direct resin composite restoration. After one year, the clinical performance of the restoration was successful. The use of sodium ascorbate gel can help the clinician to perform bonding procedures immediately after bleaching treatments.
    European journal of esthetic dentistry : official journal of the European Academy of Esthetic Dentistry, The 01/2012; 7(2):154-62.
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    ABSTRACT: The aim of this study was to assess, using the DPPH assay, the antioxidant activity of several substances that could be proposed to immediately revert the problems caused by bleaching procedures. The percentage of antioxidant activity (AA%) of 10% ascorbic acid solution (AAcidS), 10% ascorbic acid gel (AAcidG), 10% sodium ascorbate solution (SodAsS), 10% sodium ascorbate gel (SodAsG), 10% sodium bicarbonate (Bicarb), Neutralize(®) (NE), Desensibilize(®) (DES), catalase C-40 at 10 mg/mL (CAT), 10% alcohol solution of alpha-tocopherol (VitE), Listerine(®) (LIS), 0.12% chlorhexidine (CHX), Croton Lechleri (CL), 10 % aqueous solution of Uncaria Tomentosa (UT), artificial saliva (ArtS) and 0.05% sodium fluoride (NaF) was assessed in triplicate by 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) free radical assay. All substances exhibited antioxidant activity, except for CL. AAcidS, AAcidG and VitE exhibited the highest AA% (p<0.05). On the contrary, CHX, NE, LIS and NaF showed the lowest AA% (p<0.05). In conclusion, AAcidS, AAcidG, SodAsS, SodAsG and VitE presented the highest antioxidant activity among substances tested in this study. The DPPH assay provides an easy and rapid way to evaluate potential antioxidants.
    Brazilian dental journal 01/2012; 23(1):22-7.
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    ABSTRACT: The aims of this study were to evaluate the effect of resin composite (Filtek Z250 and Filtek Flow Z350) and adhesive system [(Solobond Plus, Futurabond NR (VOCO) and Adper Single Bond (3M ESPE)] on the microtensile (μTBS) and microshear bond strength (μSBS) tests on enamel, and to correlate the bond strength means between them. Thirty-six extracted human molars were sectioned to obtain two tooth halves: one for μTBS and the other one for μSBS. Adhesive systems and resin composites were applied to the enamel ground surfaces and light-cured. After storage (37(0)C/24 h) specimens were stressed (0.5 mm/min). Fracture modes were analyzed under scanning electron microscopy. The data were analyzed using two-way ANOVA and Tukey's test (α=0.05). The correlation between tests was estimated with Pearson's product-moment correlation statistics (α =0.05). For both tests only the main factor resin composite was statistically significant (p<0.05). The correlation test detected a positive (r=0.91) and significant (p=0.01) correlation between the tests. The results were more influenced by the resin type than by the adhesives. Both microbond tests seem to be positive and linearly correlated and can therefore lead to similar conclusions.
    Journal of applied oral science: revista FOB 12/2010; 18(6):591-8. · 0.39 Impact Factor
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    ABSTRACT: To determine whether the adverse interaction between a two-step/acidic etch-and-rinse adhesive (One-Step Plus [OS], Bisco) and chemically cured resin luting cement [Variolink II, Ivoclar Vivadent] can improve adhesive coupling by reducing the dentin permeability with an oxalate desensitizer (BisBlock, Bisco). After exposing dentin on the occlusal surfaces of human third molars, bonding was performed on either oxalate treated (BB) or nontreated (NB) demineralized dentin. A resin luting cement was placed in the format of a crown following the light-curing mode (only with the base syringe [LC]) or the chemically curing mode (mixture of base and catalyst syringes [CC]). The activation of the LC or CC cements was either immediately [IM], meaning soon after the placement of LC and the initial set of CC cement (5 min), or after a delay of 20 min [DP] for both modes of polymerization. Five teeth were assigned to each experimental condition. Teeth were sectioned to obtain sticks with a cross-sectional area of 0.95 mm2, which were tested using the microtensile bond strength test soon after the specified periods of polymerization. The bond strength values of each adhesive were analyzed by three-way repeated measures ANOVA and Tukey's tests (α = 0.05). Fractographic analysis of the specimens was performed using SEM. The delayed polymerization (for both LC and CC cements) produced low bond strength values compared to IM activation. When the BB was employed, the bond strength values of the CC cement was approximately doubled, while the BB did not affect the bond strength of the LC cement. Bond strength values of LC cements were higher than CC. The use of BB significantly improved the bond strength of CC cement only. The morphological observations confirmed the bond strength results. A myriad of voids could be detected in the luting cement side when BB was not applied, except for the immediately light-cured group. The use of an oxalate desensitizer (BisBlock) reduced the incompatibility between a two-step etch-and rinse adhesive and chemically cured resin cements and light-cured systems in the delayed polymerization mode.
    The journal of adhesive dentistry 11/2009; 12(5):373-9. · 0.91 Impact Factor
  • Acta odontológica venezolana 03/2009; 47(1):38-45.
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    ABSTRACT: This study evaluated the effect of a warm or cold air-dry stream for solvent evaporation on the microtensile resin-dentin bond strength (muTBS), nanoleakage pattern (SEM), degree of conversion (DC) and solvent evaporation rates (SE) of an ethanol/water- (Adper Single Bond, [SB] 3MESPE) and an acetone-based (Prime & Bond 2.1, [PB] Dentsply), two-step etch-and-rinse adhesive system. Adhesives were applied on demineralized dentin surfaces. For SE, a warm or cold air-dry stream (10 s) was applied prior to light-activation (10 s). Bonded sticks (0.8mm2) were tested in tension (0.5 mm/min). Two bonded sticks from each tooth were immersed in a 50% (w/v) solution of silver nitrate (24 h), photodeveloped (8 h) and analyzed by SEM. The DC and solvent evaporation rate of the adhesives were evaluated under FTIR and analytical balance, respectively. Data were analyzed by two-way ANOVA and Tukey test (alpha=0.05). Higher muTBS and lower nanoleakage were observed when the SE step was performed with warm air-dry stream. However, the DC of the adhesives was not altered by the use of a warm air-dry. The use of a warm air-dry stream seems to be a clinical tool to improve the bond strength and the quality of the hybrid layer (less nanoleakage infiltration), since it might reduce the number of pores within the adhesive layer.
    Journal of Dentistry 08/2008; 36(8):618-25. · 3.20 Impact Factor

Publication Stats

59 Citations
9.27 Total Impact Points

Institutions

  • 2010–2014
    • University of São Paulo
      • School of Dentistry (FO) (São Paulo)
      San Paulo, São Paulo, Brazil
  • 2008–2013
    • State University of Ponta Grossa
      • Department of Dentistry
      Ponta Grossa, Estado do Parana, Brazil
    • Universidade Luterana do Brasil
      Canoas, Rio Grande do Sul, Brazil