Publications (2)3.83 Total impact
-
Article: Improved assay method for phospholipase C.
[show abstract] [hide abstract]
ABSTRACT: A lecithin sol dispersed with deoxycholate was found to be attacked by phospholipase C in the presence of calcium ion more rapidly than were any other lecithin sols. The inorganic phosphate could be released quantitatively from the acid soluble phosphate liberated from lecithin by an excess amount of alkaline phosphatase present in phospholipase C reaction mixture. A simple and accurate assay method for phospholipase C was developed with the sol and the alkaline phosphatase.Applied microbiology 06/1967; 15(3):551-5. -
Article: Effect of the hemolysin of Pseudomonas aeruginosa on phosphatides and on phospholipase c activity.
[show abstract] [hide abstract]
ABSTRACT: The hemolysin of Pseudomonas aeruginosa was found to function as a detergent in solubilizing various phosphatides. Incorporation of the hemolysin into reaction mixtures containing phosphatides and phospholipase c significantly increased the rates of enzyme activities. Stimulation of enzyme activity was most likely due to improved dispersion of the substrates.Journal of Bacteriology 03/1967; 93(2):670-4. · 3.83 Impact Factor