Debby M Klomberg

Canisius-Wilhelmina Ziekenhuis, Nijmegen, Provincie Gelderland, Netherlands

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Publications (2)2.09 Total impact

Article: Rapid and reliable real-time PCR assay for detection of the macrolide efflux gene and subsequent discrimination between its distinct subclasses mef(A) and mef(E).

Debby M Klomberg, Hanneke A de Valk, Johan W Mouton, Corné H W Klaassen

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ABSTRACT: A real-time PCR assay is described for detection of the macrolide efflux gene, mef. Following amplification, unambiguous discrimination between the two mef subclasses, mef(A) and mef(E), is easily established using a melting curve analysis. The results of this novel assay were 100% concordant with a conventional PCR-RFLP approach but requires far less hands-on time. Furthermore, the real-time format offers semiquantitative results allowing identification of contaminated cultures and/or DNA preparations.

Journal of Microbiological Methods 03/2005; 60(2):269-73. · 2.09 Impact Factor
Article: Rapid and reliable real-time PCR assay for detection of the macrolide efflux gene and subsequent discrimination between its distinct subclasses mef(A) and mef(E)

Debby M. Klomberg, Hanneke A. de Valk, Johan W. Mouton, Corné H.W. Klaassen

[show abstract] [hide abstract]
ABSTRACT: A real-time PCR assay is described for detection of the macrolide efflux gene, mef. Following amplification, unambiguous discrimination between the two mef subclasses, mef(A) and mef(E), is easily established using a melting curve analysis. The results of this novel assay were 100% concordant with a conventional PCR-RFLP approach but requires far less hands-on time. Furthermore, the real-time format offers semiquantitative results allowing identification of contaminated cultures and/or DNA preparations.

Journal of Microbiological Methods.