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ABSTRACT: This unit provides protocols for isolating intestinal brush-border membranes from rat, pig, and cow. These membranes can be used for immunoblotting or other analytical techniques. They will also spontaneously form closed vesicles which allow for flux assays to be performed using rapid filtration techniques. Overall the isolation procedures take approximately 3.5 hr. The resulting isolated membranes can be stored under liquid nitrogen or at -70 degrees C for a week or more depending upon the species.Current protocols in cell biology / editorial board, Juan S. Bonifacino ... [et al.] 05/2006; Chapter 3:Unit 3.21.