Daniela Adorni

University Hospital of Parma, Parma, Emilia-Romagna, Italy

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Publications (5)11.09 Total impact

  • Maria Sassi · Gino Bernuzzi · Daniela Adorni · Massimo Franchini ·

    Blood transfusion = Trasfusione del sangue 01/2012; 10(4):1. DOI:10.2450/2012.0151-11 · 2.37 Impact Factor
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    Blood transfusion = Trasfusione del sangue 07/2011; 10(1):108-9. DOI:10.2450/2011.0019-11 · 2.37 Impact Factor
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    ABSTRACT: Platelet gel is being ever more frequently used to promote healing of cutaneous ulcers. However, the factors that determine the often variable clinical outcome of this procedure are still incompletely understood. The aims of this study were to demonstrate that platelet gel, even when obtained under strictly controlled conditions, produces highly variable outcomes in patients with cutaneous ulcers and to propose a method for in vitro standardisation of the biological properties of platelet gel. MATERIAL AND METHODS.: Patients were enrolled on the basis of a pre-defined protocol. Platelet concentrate was produced with standard methods, with a variability in platelet count among the different samples of less than 10%. The platelet gel for clinical use was obtained, under strictly standardized conditions, by adding thrombin and calcium gluconate to the concentrates. For in vitro studies, platelet gel, obtained from platelet-rich plasma from four donors, was frozen and thawed twice so as to increase gel contraction. The supernatant was used to modify cell proliferation, protein synthesis, and the expression of selected genes in cultures of human diploid fibroblasts. Seventeen patients (aged 44-78 years) with ulcers (4 diabetic, 11 vascular, 1 post-traumatic, 1 decubitus) were treated with platelet gel (4 autologous, 13 homologous). Complete re-epithelialisation of four ulcers (1 diabetic, 1 post-traumatic, 2 vascular) was obtained after applications of platelet gel (2 autologous, 2 homologous); in 11 other cases there was a greater than 50% reduction in the size of the ulcer. Two patients had no benefit. The supernatant of the platelet gel was able to promote dose-dependent proliferation and changes in gene expression as well as in metabolic activities related to protein synthesis. Although the use of platelet gel in the treatment of cutaneous ulcers is increasing, and conditions for its production are better standardised, very considerable variability of clinical outcomes is still observed, even within single centres, suggesting that there are differences in biological properties of platelet concentrates from individual patients which cannot be readily controlled with current techniques. The biological effects of the platelet gel supernatant described in this article may provide the basis for a simple biological validation of platelet preparations before their clinical use, so as to reduce this potentially important source of variability.
    Blood transfusion = Trasfusione del sangue 10/2010; 8(4):237-47. DOI:10.2450/2009.0118-09 · 2.37 Impact Factor
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    ABSTRACT: Cardiovascular disease, which is one of the main causes of mortality in industrialised countries, is ever increasingly the focus of prevention. In this study, called "Cardiorisk", we evaluated cardiovascular risk in the population of blood donors at the Service of Immunohaematology and Transfusion Medicine in Parma. Between January 2007 and December 2008, 6,172 consecutive blood donors (aged 35-65 years) were enrolled in this project which entailed calculating each subject's cardiovascular risk score, based on an evaluation of both unalterable risk factors (age and gender) and modifiable risk factors (total cholesterol, HDL, LDL, triglycerides, glycaemia, smoking, hypertension) as well as anti-hypertensive and/or cholesterol-lowering therapy. Of the 6,172 donors enrolled in the study, 5,039 (81.7%) had a low cardiovascular risk (score from 0-10), 774 (12.5%) had a moderate cardiovascular risk (score from 11-19) and 359 (5.8%) donors had a high cardiovascular risk (score from 20-28). In our opinion, the calculation of cardiovascular risk is an important instrument for preventive medicine in blood donors.
    Blood transfusion = Trasfusione del sangue 07/2010; 8(3):155-8. DOI:10.2450/2009.0097-09 · 2.37 Impact Factor
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    ABSTRACT: Chronic venous leg ulcers (CVLU) are chronic wounds, associated with long-standing venous hypertension, which have a poor prognosis for healing. In the process of wound healing the first step is represented by platelet aggregation and subsequent release of growth factors and other mediators, which play a key role in the repair response. Platelet gel (PG), a hemocomponent obtained by mixing platelets, thrombin, and calcium, is able, when applied topically, to release platelet mediators that likely favor CVLU healing. However, unstandardized protocols have been described in studies utilizing PG for the regeneration of a number of tissues, including CVLU; the relative clinical outcomes were hence highly variable. In our experience the topical use of PG, together with the strict adherence to the principles of good wound care, quickly promoted increased granulation tissue, followed by a complete CVLU epithelization. Although further studies and trials are needed to establish the major outcome affecting rules for optimal indications, preparation, and use of PG for CVLU treatment, PG can be undoubtedly considered a useful tool, able to improve the management of CVLU.
    Dermatologic Therapy 08/2008; 21 Suppl 1(s1):S13-7. DOI:10.1111/j.1529-8019.2008.00196.x · 1.60 Impact Factor