DONG Qianze

Publications (2)3.01 Total impact

• Source

  Available from: PubMed Central

  Article: Expression of delta-catenin is associated with progression of human astrocytoma.

  MingHao Wang, Qianze Dong, Di Zhang, YunJie Wang
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  ABSTRACT: δ-Catenin (CTNND2), which encodes a scaffold protein in humans, has been found in a few malignancies. However, the expression pattern and contribution of δ-catenin to astrocytoma progression are unclear. We investigated δ-catenin expression in human astrocytoma samples and its function in astrocytoma cell lines using immunohistochemistry, siRNA knockdown, transfection, MTT, transwell migration and Rac1 pulldown techniques. δ-Catenin protein expression was detected in cytoplasm of astrocytoma cells by immunohistochemistry. Analysis showed that grade I astrocytoma (0%, 0/11) and glial cells from normal brain tissue exhibited negative staining. δ-Catenin expression was significantly higher in grade III-IV (35%, 29/84) compared to grade II astrocytoma cells (18%, 11/61); p < 0.01). In addition, CTNND2 overexpression promoted proliferation, invasion and Rac1 activity of U251 astrocytoma cells. Treatment of δ-catenin-transfected cells with a Rac1 inhibitor decreased Rac1 activity and invasion. δ-Catenin knockdown in U87 glioblastoma cell decreased cell proliferation, invasion and Rac1 activity. The results suggest that δ-catenin expression is associated with the malignant progression of astrocytoma and promotes astrocytoma cell invasion through upregulation of Rac1 activity. δ-Catenin expression levels may serve as a useful marker of the biological behavior of astrocytoma cells.
  BMC Cancer 12/2011; 11:514. · 3.01 Impact Factor
• Article: Reduction of p120ctn isoforms is significantly associated with lymph node metastasis of lung cancer

  DONG Xinjun, LIU Yang, ZHANG Yong, DONG Qianze, MIAO Yuan, ZHANG Junyi, ZHANG Di, WANG Enhua
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  ABSTRACT: Background and objective p120ctn plays an important role in cell adhesion and has a significant association with tumorigenesis. To investigate the expression of p120ctn (p120 cateinin) isoforms in lung squamous cell carcinomas and adenocarcinomas, and analyze the correlation between p120ctn and clinicopathological parameters. Methods The expression patterns of p120ctn in lung cancer tissues and the corresponding normal lung tissues were examined by reverse transcription-polymerase chain reaction (RT-PCR). Immunofluorescence, RT-PCR, and Western blot were used to investigate the expression of p120ctn isoforms in lung cancer cell lines BE1 and LH7. Results Compared with corresponding normal lung tissues, lung cancer tissues have significantly lower levels of total mRNA, isoform1 and 3 mRNA (P<0.001, P<0.001, P=0.001). Furthermore, reduction of p120ctn isoform 1 mRNA is negatively associated-whereas p120ctn isoform 3 is positively associated-with lymphatic metastasis (P=0.01, P=0.029). BE1 cells with 94% metastatic frequency has lower levels of p120ctn isoforms than LH7 cells with low metastatic potential. Conclusion Reductions of p120ctn isoform 1 and 3 mRNA is a common phenomenon in lung cancer tissues and may play a role in metastasis progression of human lung cancer.
  Chinese Journal of Lung Cancer. 01/2008;