I J Sinclair

Le ministère de l'Agriculture, des Pêcheries et de l'Alimentation du Québec, Québec, Quebec, Canada

Are you I J Sinclair?

Claim your profile

Publications (14)27.07 Total impact

  • I J Sinclair, D W Tarry
    The Veterinary record 07/1993; 132(24):613. DOI:10.1136/vr.132.24.613 · 1.63 Impact Factor
  • D W Tarry, I J Sinclair, D A Wassall
    [Show abstract] [Hide abstract]
    ABSTRACT: The British warble fly eradication programme has resulted in the numbers of infested cattle falling from an estimated four million in 1978 to zero in 1991. In 1982, when about 705 herds were infested the disease was made notifiable, and serological surveillance was begun in 1988. In spring 1991, 227,000 blood samples were tested by using the ELISA technique and no warbled animals were found. In addition no clinical cases were reported during 1991.
    The Veterinary record 11/1992; 131(14):310-2. DOI:10.1136/vr.131.14.310 · 1.63 Impact Factor
  • G C Coles, D W Tarry, I.J.B. Sinclair
    Parasitology Today 08/1992; 8(7):236; author reply 236. DOI:10.1016/0169-4758(92)90122-I · 5.51 Impact Factor
  • I J Sinclair, D W Tarry, D A Wassall
    The Veterinary record 10/1990; 127(11):285. DOI:10.1136/vr.127.11.285 · 1.63 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Sera from 152,434 cattle held on 6175 farms in England and Wales were tested for the presence of antibodies to Hypoderma bovis during the 10 weeks starting on February 6, 1989. Thirty-nine positive animals (25.6 animals/100,000 tested) were identified on 21 of the farms (0.34 per cent). In comparison, the survey in 1988 examined 74,502 cattle on 3087 farms and found 29 positive animals (38.9/100,000 tested) on 18 farms (0.58 per cent).
    The Veterinary record 05/1990; 126(14):327-9. DOI:10.1136/vr.126.14.327 · 1.63 Impact Factor
  • I J Sinclair, D W Tarry, D A Wassall
    [Show abstract] [Hide abstract]
    ABSTRACT: Sera from 74,502 cattle from 3087 farms in England and Wales were tested for the presence of antibodies against Hypoderma bovis in the spring of 1988. Twenty-nine positive sera were identified on 18 premises and these animals were treated; an examination of 6030 sera taken from 108 neighbouring herds identified another 17 seropositive animals on 10 farms in Devon, Cornwall, Lancashire, Shropshire and Powys, indicating that these counties still harbour populations of warble fly.
    The Veterinary record 04/1989; 124(10):243-4. DOI:10.1136/vr.124.10.243 · 1.63 Impact Factor
  • I J Sinclair, D A Wassall
    [Show abstract] [Hide abstract]
    ABSTRACT: An antigen was prepared from metabolic products which were produced by maintaining Fasciola hepatica adults in RPMI tissue culture medium for 24 h. The antigen compared favourably with a soluble extract of adult flukes when used in an enzyme-linked immunosorbent assay (ELISA). Experimentally infected calves were used to evaluate the sensitivity of the test and a survey from areas of both traditionally high and low incidence of the disease was carried out. Evidence is presented which suggests that this metabolic antigen could be used for the sero diagnosis of naturally occurring infections. In addition the use of a microcomputer to read, file and analyse the results enabled a very large number of samples to be processed daily.
    Veterinary Parasitology 04/1988; 27(3-4):283-90. DOI:10.1016/0304-4017(88)90043-X · 2.55 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: An enzyme-linked immunosorbent assay has been shown to detect antibodies in the circulation of sheep infected with Psoroptes ovis. Strong positive reactions were obtained from 19 sheep with four-month-old infections. No cross reaction was observed with sera obtained from sheep infected with either Fasciola hepatica, Nematodirus battus, Ostertagia circumcincta or Damalinia ovis.
    Research in Veterinary Science 08/1987; 43(1):34-5. · 1.51 Impact Factor
  • D A Wassall, I J Sinclair
    [Show abstract] [Hide abstract]
    ABSTRACT: Electrofocusing of F. hepatica adult antigen in granulated gels separated the material into 22 arbitrary fractions. Polyacrylamide gel analysis demonstrated groups of proteins with similar iso-electric points in 19 of the fractions. A microplate ELISA detected antigens in all 22 fractions and was used to test the serum antibody response in infected rats, rabbits, lambs and calves to these antigen fractions. The results indicated that rat and calf sera gave a much stronger response than rabbit and lamb sera to the antigens which separated above pH 7.0. It is possible that the greater efficiency of the rat and bovine immune systems in combating re-infection with F. hepatica may be related to this response.
    Parasite Immunology 08/1985; 7(4):359-66. · 1.85 Impact Factor
  • D. A. WASSALL, I. J. SINCLAIR
    [Show abstract] [Hide abstract]
    ABSTRACT: Electrofocussing of F. hepatica adult antigen in granulated gels separated the material into 22 arbitrary fractions. Polyacrylamide gel analysis demonstrated groups of proteins with similar iso-electric points in 19 of the fractions. A microplate ELISA detected antigens in all 22 fractions and was used to test the serum antibody response in infected rats, rabbits, lambs and calves to these antigen fractions. The results indicated that rat and calf sera gave a much stronger response than rabbit and lamb sera to the antigens which separated above pH 7·0. It is possible that the greater efficiency of the rat and bovine immune systems in combating re-infection with F. hepatica may be related to this response.
    Parasite Immunology 07/1985; 7(4):359-366. DOI:10.1111/j.1365-3024.1985.tb00082.x · 1.85 Impact Factor
  • I J Sinclair, D A Wassall, R J Cawthorne
    [Show abstract] [Hide abstract]
    ABSTRACT: A peroxidase anti-peroxidase immunohistochemical technique was used to demonstrate IgA-containing cells in the small intestine of lambs infected with Nematodirus battus. These cells were more numerous in the infected lambs than in the uninfected animals. The difference was greatest for the first three sites, 1 to 3 m distal to the pylorus, where the preponderance of the N battus infection is found. It is suggested that future immunohistochemical studies on the relationships between IgA and resistance to N battus should be directed to this area.
    Research in Veterinary Science 06/1985; 38(3):373-6. · 1.51 Impact Factor
  • I J Sinclair, D W Tarry, D A Wassall
    [Show abstract] [Hide abstract]
    ABSTRACT: The antibody titre of seven calves, naturally infected with Hypoderma bovis, was estimated by an enzyme-linked immunosorbent assay (ELISA) on sera collected from June to early December 1983 following infection in 1982. It was found that the antibody was lost from the circulation of these animals about 14 weeks after the disappearance of the infection. It is suggested that the presence of antibody in the sera of calves sampled in the late autumn and winter indicated that a new infection had been acquired.
    Research in Veterinary Science 12/1984; 37(3):383-4. · 1.51 Impact Factor
  • I J Sinclair, D A Wassall
    [Show abstract] [Hide abstract]
    ABSTRACT: An adaptation of the enzyme-linked immunosorbent assay has been shown to detect antibodies in the circulation of cattle infected with Hypoderma bovis. Strong positive reactions were obtained from all infected cattle, even those which harboured only one larva. A strong cross reaction was observed in sera taken from cattle infected with H lineatum, but not in cattle infected with Fasciola hepatica or Ostertagia ostertagi.
    Research in Veterinary Science 04/1983; 34(2):251-2. · 1.51 Impact Factor
  • I J Sinclair, D A Wassall
    [Show abstract] [Hide abstract]
    ABSTRACT: Rats were given either one or two infections of F. hepatica and were killed between 0 and 58 days after the final infection. Antibody to F. hepatica was demonstrated in the serum by the Enzyme-linked Immunosorbent Assay (ELISA) and the Ouchterlony double-diffusion precipitin test in all rats which received two infections, but only ELISA detected antibody in rats before the 15th day of a single infection. Three to nine days after a second infection, the area round the liver capsule was shown by peroxidase anti-peroxidase staining (PAP) to contain an increased concentration of IgG. It is suggested that this IgG may play a role in the immune mechanism of the rat.
    Journal of Comparative Pathology 11/1981; 91(4):573-9. DOI:10.1016/0021-9975(81)90086-4 · 1.10 Impact Factor