Biochemistry (Moscow) 05/2012; 76(9):1075-1077. · 1.06 Impact Factor
ABSTRACT: This review covers data on composition and structure of lipid A, core, and O-polysaccharide of the known lipopolysaccharides from Gram-negative bacteria. The relationship between the structure and biological activity of lipid A is discussed. The data on roles of core and O-polysaccharide in biological activities of lipopolysaccharides are presented. The structural homology of some oligosaccharide sequences of lipopolysaccharides to gangliosides of human cell membranes is considered.
Biochemistry (Moscow) 04/2010; 75(4):383-404. · 1.06 Impact Factor
ABSTRACT: The role of erythrocytic surface membrane proteins and membrane charge in the interactions of the erythrocytes with lipopolysaccharides
(LPS) isolated from Escherichia coli O55:B5 (LPS
, S-form) has been examined by two independent methods, flow cytometry and cell electrophoresis. Treatment of erythrocytes
with trypsin that modifies stereochemical properties of cell surface resulted in a 16% increase in the level of the erythrocyte
fluorescence measured after their incubation with fluorescently labeled LPS
. Electrophoretic mobility (EM) of the trypsin-treated erythrocytes was reduced by 16%. The removal of sialic acids from the
erythrocyte surface with neuraminidase had no considerable effect either on the relative EM values or fluorescence intensity
after the incubation of cells with LPS. The results suggest that the major role in the incorporation of the S-form LPS into
the membrane of human erythrocytes is played by stereochemical factors, whereas the cell surface charge is less significant.
Biochemistry (Moscow) Supplement Series A Membrane and Cell Biology 05/2008; 2(2):128-132.
Doklady Biological Sciences 402:189-91.