C Jamet-Vierny

Université Paris-Sud 11, Paris, Ile-de-France, France

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Publications (9)54.33 Total impact

  • Corinne Jamet-Vierny, Robert Debuchy, Magali Prigent, Philippe Silar
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    ABSTRACT: Components involved in the activation of the MAPK cascades in filamentous fungi are not well known. Here, we provide evidence that IDC1, a pezizomycotina-specific gene is involved along with the PaNox1 NADPH oxidase in the nuclear localization of the PaMpk1 MAP kinase, a prerequisite for MAPK activity. Mutants of IDC1 display the same phenotypes as mutants in PaNox1 and PaMpk1, i.e., lack of pigment and of aerial hyphae, female sterility, impairment in hyphal interference and inability to develop Crippled Growth cell degeneration. As observed for the PaNox1 mutant, IDC1 mutants are hypostatic to PaMpk1 mutants. IDC1 seems to play a key role in sexual reproduction. Indeed, fertility is diminished in strains with lower level of IDC1. In strains over-expressing IDC1, protoperithecia reach a later stage of development towards perithecia without fertilization; however, upon fertilization maturation of fertile perithecia is diminished and delayed. In addition, heterokaryon construction shows that IDC1 is necessary together with PaNox1 in the perithecial envelope but not in the dikaryon resulting from fertilization.
    Fungal Genetics and Biology 01/2008; 44(12):1219-30. · 3.26 Impact Factor
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    ABSTRACT: Senescence of Podospora anserina is triggered by a cytoplasmic and infectious factor (the determinant of senescence) and is always correlated with mitochondrial DNA modifications, especially with the accumulation of small circular subgenomic DNA molecules, the senDNAs. Several observations have suggested that the senDNAs could be the cytoplasmic and infectious determinant. However, we show here (1) that senDNA molecules can be transferred to a young culture without the cotransmission of the determinant of senescence and (2) that the determinant of senescence does not segregate as a mitochondrial DNA mutation. Overall, our data strongly argue that amplification of senDNA molecules in the mitochondria is not an intrinsic property of these small DNA molecules. They question the nature of the actual determinant of senescence.
    Fungal Genetics and Biology 07/1999; 27(1):26-35. · 3.26 Impact Factor
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    C Jamet-Vierny, V Contamine, J Boulay, D Zickler, M Picard
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    ABSTRACT: Tom70 and Mdm10 are mitochondrial outer membrane proteins. Tom70 is implicated in the import of proteins from the cytosol into the mitochondria in Saccharomyces cerevisiae and Neurospora crassa. Mdm10 is involved in the morphology and distribution of mitochondria in S. cerevisiae. Here we report on the characterization of the genes encoding these proteins in the filamentous fungus Podospora anserina. The two genes were previously genetically identified through a systematic search for nuclear suppressors of a degenerative process displayed by the AS1-4 mutant. The PaTom70 protein shows 80% identity with its N. crassa homolog. The PaMdm10 protein displays 35.9% identity with its S. cerevisiae homolog, and cytological analyses show that the PaMDM10-1 mutant exhibits giant mitochondria, as does the S. cerevisiae mdm10-1 mutant. Mutations in PaTOM70 and PaMDM10 result in the accumulation of specific deleted mitochondrial genomes during the senescence process of the fungus. The phenotypic properties of the single- and double-mutant strains suggest a functional relationship between the Tom70 and Mdm10 proteins. These data emphasize the role of the mitochondrial outer membrane in the stability of the mitochondrial genome in an obligate aerobe, probably through the import process.
    Molecular and Cellular Biology 12/1997; 17(11):6359-66. · 5.04 Impact Factor
  • C Jamet-Vierny, J Boulay, J F Briand
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    ABSTRACT: The unavoidable senescence process that limits the vegetative growth of Podospora anserina is always associated with an accumulation of various classes of circular, tandemly arranged, defective mitochondrial DNA molecules (senDNAs). The monomers of the senDNAs belonging to the so-called beta class share a common core, but differ in both their length and termini. To understand the mechanism leading to their formation, we have determined the junction sequence of 36 senDNA beta monomers present in various senescent cultures. In most cases, we observe that: (1) short direct repeats precisely bound the senDNA beta termini and (2) one copy of the repeats is retained in the senDNA sequence. Moreover, PCR analysis of the mitochondrial DNA of some of the senescent cultures, has allowed us to detect another genome which is exactly lacking the sequence of the senDNA beta found in the culture. These results demonstrate that an intramolecular unequal cross-over occurring between short direct repeats can generate deleted mtDNA molecules in P. anserina. In addition, the polymorphism displayed by one pair of repeats allows us to establish that this cross-over may be associated with a short conversion tract spanning a few (about 15) nucleotides.
    Current Genetics 03/1997; 31(2):162-70. · 2.41 Impact Factor
  • C Jamet-Vierny, J Boulay, O Begel, P Silar
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    ABSTRACT: The unavoidable arrest of vegetative growth in Podospora anserina (senescence process) is always correlated with rearrangements of the mitochondrial chromosome, mainly consisting in the amplification of particular regions as tandemly repeated circular molecules (senDNAs). One sequence systematically amplified in senescent cultures corresponds precisely to the first intron (intron alpha) of the cox1 gene; nevertheless, other regions (called beta and gamma) are also frequently amplified. The experiments presented in this paper show that cellular death is in some cases associated with the sole presence of large amounts of senDNA beta. In addition, we provide evidence that senDNA beta and senDNA alpha accumulate by different mechanisms, as previously proposed. This suggests that beta senDNAs have a lethal effect on the mycelium on their own and most likely have replicative properties independent of the presence of sequence alpha. These data do not fit well with the current opinion that gives an essential role to intron alpha in the senescence of P. anserina.
    Current Genetics 03/1997; 31(2):171-8. · 2.41 Impact Factor
  • C Jamet-Vierny, E Shechter
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    ABSTRACT: In Podospora anserina the phenomenon of senescence was previously shown to be correlated with the presence of senescence-specific circular DNAs (senDNAs), resulting from the amplification of distinct regions (alpha, beta, gamma and epsilon) of the mitochondrial chromosome. The beta region gives rise to senDNAs with variable sizes, but sharing a 1-kb common sequence. Here, we present a molecular analysis of five beta senDNAs. We have determined the nucleotide sequence around the circularization site of each senDNA monomer. In two cases, the presence of a tRNA gene, very close to the 3' end of the monomer, has been observed. This suggests that some beta senDNAs could be generated via a reverse transcription step. We have furthermore shown that the beta senDNAs produce specific transcripts which undergo normal processing of their introns. We propose that a transcription start site, located in the beta common region, is involved in mitochondrial replication allowing the amplification of the beta senDNAs.
    Current Genetics 07/1994; 25(6):538-44. · 2.41 Impact Factor
  • C Jamet-Vierny, O Begel, L Belcour
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    ABSTRACT: By DNA sequencing and hybridization experiments we have localized the genes cob and col on the mitochondrial chromosome of Podospora anserina. The positions we have determined for these two genes are different from those previously attributed to them. The presence in the gene col of at least two introns, belonging respectively to class I and II, has been demonstrated. This gene, with a size of about 20 X 10(3) bases, appears to be the longest known mitochondrial mosaic gene.
    European Journal of Biochemistry 10/1984; 143(2):389-94. · 3.58 Impact Factor
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    Corinne JAMET-VIERNY, Odile BEGEL, Leon BELCOUR
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    ABSTRACT: By DNA sequencing and hybridization experiments we have localized the genes cob and col on the mitochondrial chromosome of Podospora anserina. The positions we have determined for these two genes are different from those previously attributed to them. The presence in the gene col of at least two introns, belonging respectively to class I and II, has been demonstrated. This gene, with a size of about 20 × 103 bases, appears to be the longest known mitochondrial mosaic gene.
    European Journal of Biochemistry - EUR J BIOCHEM. 09/1984; 143(2):389-394.
  • C Jamet-Vierny, O Begel, L Belcour
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    ABSTRACT: Senescence in Podospora anserina has long been shown to be under cytoplasmic control. Comparison of DNAs isolated from young and senescent cultures made it possible to detect the presence, in senescent cultures only, of a specific DNA (SEN-DNA). This DNA consists of repeated sequences arranged in a multimeric set of circular molecules. In this study we have examined one particular SEN-DNA whose monomer unit is 6300 bp long. Using the Southern hybridization technique, we have demonstrated that this SEN-DNA results from the amplication of a sequence of the mitochondrial chromosome. This amplification, which resembles the process leading to rho- ("petite") mutations in yeast, is discussed in relation to the determinism of senescence.
    Cell 09/1980; 21(1):189-94. · 31.96 Impact Factor

Publication Stats

182 Citations
54.33 Total Impact Points

Institutions

  • 1999–2008
    • Université Paris-Sud 11
      • Institut de Génétique et Microbiologie (IGMORS)
      Paris, Ile-de-France, France
  • 1994–1997
    • French National Centre for Scientific Research
      • Centre de génétique moléculaire
      Paris, Ile-de-France, France