Shaoping Deng,
Daniel J Moore,
Xiaolun Huang,
Moh-Moh Lian,
Muhammad Mohiuddin,
Ergun Velededeoglu,
Major K Lee,
Samsher Sonawane,
James Kim,
Jing Wang,
Haiying Chen,
Steven A Corfe, Christopher Paige,
Mark Shlomchik,
Andrew Caton,
James F Markmann
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ABSTRACT: Selective interference with the CD45RB isoform by mAb (anti-CD45RB) reliably induces donor-specific tolerance. Although previous studies suggest participation of regulatory T cells, a mechanistic understanding of anti-CD45RB-induced tolerance is lacking. We report herein the unexpected finding that tolerance induced by this agent is not established in B cell-deficient mice but can be recovered by preemptive B lymphocyte transfer to B cell-deficient hosts. Using B cells from genetically modified donors to reconstitute B cell-deficient recipients, we evaluate the role of B lymphocyte-expressed CD45RB, T cell costimulatory molecules, and the production of Abs in this novel tolerance mechanism. Our data document an Ab-induced tolerance regimen that is uniquely B lymphocyte-dependent and suggest mechanistic contributions to tolerance development from the B cell compartment through interactions with T cells.
The Journal of Immunology 06/2007; 178(10):6028-32. · 5.79 Impact Factor
