Are you Cheng-Chih P Fan-Chiang?

Claim your profile

Publications (2)12.11 Total impact

  • Source
    John H Miller · Cheng-Chih P Fan-Chiang · T P Straatsma · Michael A Kennedy ·
    [Show abstract] [Hide abstract]
    ABSTRACT: Molecular dynamics (MD) simulations were carried out on the DNA oligonucleotide GGGAACAACTAG:CTAGTTGTTCCC in its native form and with guanine in the central G(19):C(6) base pair replaced by 8-oxoguanine (8oxoG). A box of explicit water molecules was used for solvation, and Na(+) counterions were added to neutralize the system. The direction and magnitude of global bending were assessed by a technique used previously to analyze simulations of DNA containing a thymine dimer. The presence of 8oxoG did not greatly affect the magnitude of DNA bending; however, bending into the major groove was significantly more probable when 8oxoG replaced G(19). Crystal structures of glycosylases bound to damaged-DNA substrates consistently show a sharp bend into the major groove at the damage site. We conclude that changes in bending dynamics that assist the formation of this kink are a part of the mechanism by which glycosylases of the base excision repair pathway recognize the presence of 8oxoG in DNA.
    Journal of the American Chemical Society 06/2003; 125(20):6331-6. DOI:10.1021/ja029312n · 12.11 Impact Factor
  • Source
    Cheng-Chih Fan-Chiang · Robert R Lewis ·