Carlos Gil-Turnes

Publications (6)10.57 Total impact

• Article: Production and characterization of Clostridium perfringens recombinant β toxoid.

  Adalgisa Milach, João Rodrigo Gil de los Santos, Carlos Gil Turnes, Angela Nunes Moreira, Ronnie Antunes de Assis, Felipe Masiero Salvarani, Francisco Carlos Faria Lobato, Fabricio Rochedo Conceição
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  ABSTRACT: In this work, we produced and evaluated a vaccine based on a β toxoid of Clostridium perfringens type C produced in Escherichia coli (rBT). The non-toxic rBT was innocuous for mice and induced 14 IU mL(-1) of β antitoxin in rabbits, complying with the European Pharmacopeia and CFR9 - USDA guidelines.
  Anaerobe 01/2012; 18(3):363-5. · 2.41 Impact Factor
• Article: Bioremediation of Parboiled Rice Effluent Supplemented with Biodiesel-Derived Glycerol Using Pichia pastoris X-33.

  Diego Gil de Los Santos, Carlos Gil Turnes, Fabricio Rochedo Conceição
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  ABSTRACT: This paper describes the use of Pichia pastoris X-33 as a bioremediator to reduce the chemical oxygen demand (COD), total Kjeldahl nitrogen (TKN), and phosphorus (P-PO(4) (   3-)), after culture in parboiled rice effluent supplemented with p.a. glycerol or a glycerol by-product of the biodiesel industry. The greatest reduction in the COD (55%), TKN (45%), and P-PO(4) (   3-) (52%) of the effluent was observed in cultures of P. pastoris X-33 supplemented with 15 g ·L(-1) of biodiesel-derived glycerol. Furthermore, the overall biomass yield was 2.1 g ·L(-1). These data suggest that biodiesel-derived glycerol is an efficient carbon source for the bioremediation of parboiled rice effluent and biomass production.
  TheScientificWorldJOURNAL 01/2012; 2012:492925. · 1.66 Impact Factor
• Article: Evaluation in broilers of the probiotic properties of Pichia pastoris and a recombinant P. pastoris containing the Clostridium perfringens alpha toxin gene.

  João Rodrigo Gil de los Santos, Otávio Brod Storch, Cristina Gevehr Fernandes, Carlos Gil-Turnes
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  ABSTRACT: The probiotic properties of Pichia pastoris and of a recombinant P. pastoris containing the Clostridium perfringens alpha toxin gene were evaluated in broilers. One-day-old chicks randomly divided in four groups were fed with commercial feed devoid of antibacterials. The control group (1) received plain food, while the other groups were supplemented with either P. pastoris (2), the recombinant P. pastoris (3) or Bacillus cereus var. Toyoi (4). At day 49, live weights, feed efficiency and seroconversions were higher (P<0.05) in the supplemented groups than in the control groups. Group 3 showed the best results, while group 2 had lower weight gain than groups 3 and 4 although food conversion was better than in group 4. Seroconversions were not different (P>0.05) among the supplemented groups. Adverse reactions were not observed in histopathologic evaluation. We concluded that P. pastoris and the recombinant P. pastoris could be used as probiotics in broilers.
  Veterinary Microbiology 12/2011; 156(3-4):448-51. · 3.33 Impact Factor
• Article: Molecular diversity of Moraxella bovis isolated from Brazil, Argentina and Uruguay over a period of three decades.

  Fabricio Rochedo Conceição, Odir Antônio Dellagostin, Fernando Paolichi, Analia Cobo Leturia, Carlos Gil Turnes
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  ABSTRACT: The molecular profile of 30 Moraxella bovis strains, recovered from outbreaks of infectious bovine keratoconjunctivitis in Argentina, Brazil and Uruguay between 1974 and 2001, was determined through randomly applied polymorphic DNA (RAPD) analysis. Molecular profiles of nine strains recovered after 1990 varied from those recovered before 1990. The profiles of 13 strains (48%) differed from those of three vaccinal strains extensively used since 1984 in Argentina and Uruguay. Eight Argentinean strains, one from Brazil and two from Uruguay had identical RAPD profiles. Strains belonging to different serogroups had identical RAPD profiles, demonstrating that this technique was not able to discriminate among strains with low cross-reactivity indices. RAPD may be helpful in the primary characterization of M. bovis strains, but it does not replace serological characterization.
  The Veterinary Journal 02/2004; 167(1):53-8. · 2.24 Impact Factor
• Article: Antigenic relationships of Moraxella bovis isolates recovered from outbreaks of infectious bovine keratoconjunctivitis in Argentina, Brazil, and Uruguay between 1983 and 2000.

  Fabrício Rochedo Conceição, Fernando Paolicchi, Ana Lia Cobo, Carlos Gil-Turnes
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  ABSTRACT: Cross-reactivity indices (CRIs) of 28 isolates of Moraxella bovis recovered from outbreaks of infectious bovine keratoconjunctivitis in Argentina (A, 11 isolates), Brazil (B, 7), and Uruguay (U, 10) between 1983 and 2000 were estimated. Hyperimmune sera were produced in rabbits and antibody titres determined with each isolate. Isolates showing CRIs3 70 were placed in the same group. Group I had 13 isolates (A, 1; B, 6; U, 6); group II had 6 isolates (A, 4; U, 2); groups III, IV, and V had 2 isolates each, recovered in Argentina; group VI had 2 isolates, from Uruguay; and group VII had 1 isolate, from Brazil. The CRIs3 70 between vaccine strains and isolates recovered before and after 1990 were 58% and 42%, 50% and 50%, and 33% and 67% with vaccine strains 2419, 2358, and 2439, respectively. Isolate 273, from Uruguay, showed CRIs > 70 with 78% of the isolates and is recommended as the vaccine strain.
  Canadian journal of veterinary research = Revue canadienne de recherche vétérinaire 11/2003; 67(4):315-8. · 0.94 Impact Factor
• Source

  Available from: unirioja.es

  Article: Enterite necrótica aviária

  João Rodrigo Gil de Los Santos, Fabricio Rochedo Conceição, Carlos Gil-Turnes
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  ABSTRACT: A Enterite Necrótica Aviária (ENA) é uma enterotoxemia aguda que aparece subitamente e provoca morte rápida, afetando principalmente animais jovens. Embora seu impacto negativo na produção, devido ao aumento da conversão alimentar e da condenação de carcaças seja já conhecido, questões relacionadas à etiologia, à patogenia e ao controle desta importante enfermidade necessitam de maiores esclarecimentos. Nos últimos anos, o controle da ENA baseou-se na aplicação de antibióticos na ração animal, prática banida pelo mercado consumidor, que exigiu o desenvolvimento de novas estratégias de controle. Esta revisão aborda informações sobre a etiologia, a epizootiologia, a patogenia, o diagnóstico e o controle da doença, em especial a utilização de probióticos e vacinas como alternativas de controle da ENA
  Ciencia rural, ISSN 0103-8478, Vol. 38, Nº. 7, 2008, pags. 2076-2082.