ABSTRACT: To apply the bioinformatics tools for analyzing the differentially expressed genes in oral submucous fibrosis (OSF) to obtain the implied biological significance.
By using DAVID and Onto-express bioinformatic tools, 865 differentially expressed genes in OSF were analyzed and the analysis of chromosome location, gene ontology (GO) and genetic-association diseases were performed.
A majority of the differentially expressed genes were located on chromosome 1,2,5,6,7,11,12 (P < 0.01). GO classification of the differentially expressed genes identified the biological process subgroups, including genes involved in immune response, defense response and so on. The cellular component subgroups were associated with extracellular matrix, cytoskeleton and membrane, molecular function subgroups related to protein binding, extracellular matrix structural constituent and signal transducer activity. The diseases genetically associated with these genes included infection, immune and cardiovascular diseases.
Bioinformatics can provide the quick and parallel analysis of massive data got from gene microarrays and enable the function classification of the differentially expressed genes, which provides new ideas on the research of pathogenesis and epidemiology of OSF.
Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology 04/2008; 43(3):168-71.
ABSTRACT: To culture human ginginval epithelia in vitro, and to construct the tissue engineered gingiva with the acellular dermal matrix (ADM).
Human ginginval epithelia were isolated from the gingival tissue, and the cells were cultured and seeded onto the surface of ADM. After 7 days of submerged incubation, an air-liquid interface culture was performed for 7, 14, and 21 days. The complex constructed above was taken for histological examination.
Human ginginval epithelia could proliferate well on the surface of ADM, and form multilayer structure. But the superficial epithelium was partially keratinized.
Tissue engineered gingiva may be constructed with human ginginval epithelia and ADM in vitro.
Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences 11/2007; 32(5):796-9.
ABSTRACT: To evaluate the clinical effects of garlic plaster on the recurrent oral ulcer (ROU).
The garlic powder was made by special method and made into garlic plaster including 0.1% garlicin. The plaster was painted on the surface of ROU to examine its clinical effect. The patients were followed up for 1 to 4 years.
Of the 30 patients treated with garlic plaster, the complete effective rate was 83.3%, the partial effective rate was 16.7%, and the total effective rate was 100%. Fourteen patients did not recur 4 years after the treatment (46.7%).
Garlic has a broad clinical prospect due to its effective treatment on ROU.
Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences 07/2004; 29(3):330-1.