Bettina Golecki

Landeslabor Schleswig-Holstein, Neumünster, Schleswig-Holstein, Germany

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Publications (6)20.21 Total impact

  • Angelika Ziegler, Bettina Golecki, Ute Kastirr
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    ABSTRACT: Sequence analysis has shown that diseased wheat plants in Northern Germany were infected with the New York strain of soil-borne wheat mosaic virus (SBWMV). This is in contrast to the only other confirmed site of SBWMV occurring in Germany, where a variant closely related to the Nebraska-type strain of SBWMV was found. The results indicate that there have been at least two separate introductions of SBWMV strains to Germany. A survey is required to study the actual distribution of SBWMV in Germany.
    Journal of Phytopathology 04/2013; 161(4):290-292. · 1.00 Impact Factor
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    ABSTRACT: Zusammenfassung Erhebungen zum Vorkommen bodenbürtiger Viren in Schleswig-Holstein zeigten, dass in diesem Bundesland das Soil-borne wheat mosaic virus (SBWMV) in Weizen-und Roggenanbaugebieten verbreitet ist. Während in Süddeutschland der Nebraska (N)-Stamm dieses Virus vorkommt, tritt in Norddeutschland der New York (NY)-Stamm auf. In den Infektionsherden der Getreidefelder werden verschiedene Winterweizensorten stark geschädigt. Um Anbauempfehlungen für die Kultivierung virusresistenten Weizens in Befallsregionen geben zu können, wurden ausgewählte Sorten unter Gewächshausbedingungen in infektiöser Erde eines betroffenen Standortes auf Resistenz getestet. Weiterhin erfolgte die Identifizierung des diagnostischen Markers Xgwm469-5D (153 bp-oder 155 bp-Allel) für das Sbm1-Resistenzgen gegen Furoviren im geprüften Weizenmaterials. Im Ergebnis dieser Untersuchungen können für den Weizenanbau in mit SBWMV-NY verseuchten Flächen die Sorten Farandole, Hyland, Hybery, Mirage und Carenius empfohlen werden. Abstract The study of soil-borne viruses in Schleswig-Holstein shows an increasing distribution of Soil-borne wheat mosaic virus (SBWMV) in wheat and rye growing areas in this federal state of Germany. Whereas the Nebraska (N) strain of this virus was found in Southern Germany, the New York (NY) strain is present in Northern Germany. Selective wheat cultivars were screened for resistance to this virus under greenhouse conditions in an infested soil sample with the aim to develop recommendations for growers. Furthermore, the identification of the diagnostic marker Xgwm469-5D (153 bp-or 155 bp-allel) for the Sbm1-resistance gene against furoviruses was carried out in the wheat material under investigation. As a result of our work, the cultivation of the wheat cultivars Farandole, Hyland, Hybery, Mirage and Carenius is recommended for SBWMV-NY affected fields.
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    ABSTRACT: Parasitism of the cyst nematode Heterodera schachtii is characterized by the formation of syncytial feeding structures in the host root. Syncytia are formed by the fusion of root cells, accompanied by local cell wall degradation, fusion of protoplasts and hypertrophy. Expansins are cell wall-loosening proteins involved in growth and cell wall disassembly. In this study, we analysed whether members of the expansin gene family are specifically and developmentally regulated during syncytium formation in the roots of Arabidopsis thaliana. We used PCR to screen a cDNA library of 5-7-day-old syncytia for expansin transcripts with primers differentiating between 26 alpha- and three beta-expansin cDNAs. AtEXPA1, AtEXPA3, AtEXPA4, AtEXPA6, AtEXPA8, AtEXPA10, AtEXPA15, AtEXPA16, AtEXPA20 and AtEXPB3 could be amplified from the library. In a semi-quantitative RT-PCR and a Genechip analysis AtEXPA3, AtEXPA6, AtEXPA8, AtEXPA10 and AtEXPA16 were found to be upregulated specifically in syncytia, but not to be transcribed in surrounding root tissue. Histological analyses were performed with the aid of promoter::GUS lines and in situ RT-PCR. Results from both approaches supported the specific expression pattern. Among the specifically expressed genes, AtEXPA3 and AtEXPA16 turned out to be of special interest as they are shoot-specific in uninfected plants. We conclude that syncytium formation involves the specific regulation of expansin genes, indicating that the encoded expansins take part in cell growth and cell wall disassembly during syncytium formation.
    The Plant Journal 11/2006; 48(1):98-112. · 6.58 Impact Factor
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    B Golecki, A Schulz, G A Thompson
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    ABSTRACT: Phloem-specific proteins (P proteins) are particularly useful markers to investigate long-distance trafficking of macromolecules in plants. In this study, genus-specific molecular probes were used in combination with intergeneric grafts to reveal the presence of a pool of translocatable P protein subunits. Immunoblot analyses demonstrated that Cucurbita spp P proteins PP1 and PP2 are translocated from Cucurbita maxima stocks and accumulate in Cucumis sativus scions. Cucurbita maxima or Cucurbita ficifolia PP1 and PP2 mRNAs were not detected in Cucumis sativus scions by either RNA gel blot analysis or reverse transcription-polymerase chain reaction, indicating that the proteins, rather than transcripts, are translocated. Tissue prints of the Cucumis sativus scion, using antibodies raised against Cucurbita maxima PP1 or PP2, detected both proteins in the fascicular phloem of the stem at points distal to the graft union and in the petiole of a developing leaf, suggesting that the proteins move within the assimilate stream toward sink tissues. Cucurbita maxima PP1 was immunolocalized by light microscopy in sieve elements of the extrafascicular phloem of Cucumis sativus scions, whereas Cucurbita maxima PP2 was detected in both sieve elements and companion cells.
    The Plant Cell 02/1999; 11(1):127-40. · 9.25 Impact Factor
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    ABSTRACT: Interspecific and intergeneric grafts of Cucurbitaceae were used to study the mobility of structural P-proteins in the phloem. When Cucumis sativus L. scions were grafted onto Cucurbita rootstocks, at least nine additional proteins appeared on sodium dodecyl sulfate-polyacrylamide electrophoresis gels of scion exudate, 9–11 d after grafting. These proteins corresponded exactly to those of the respective Cucurbita sp. rootstock, including the filament-forming phloem protein PP1 and the phloem lectin PP2, as shown by the apparent molecular weights and peptide maps. According to probing at three sites, the additional proteins were evenly distributed within the scion. The appearance of additional proteins was correlated with the establishment of phloem bridges across the graft union. The developmental coincidence establishes that the structural proteins or their precursors are translocated in the phloem. This translocation was a universal phenomenon in Cucurbitaceae as shown by a comparative screening for additional proteins in eleven graft combinations, using Benincasa hispida (Thunb.) Cogn., Citrullus colocynthis (L.) Schrad., Cucumis melo L, C. sativus, Cucurbita ficifolia Bouché, Cucurbita maxima Duchesne ex Lam., and Trichosanthes cucumerina var. lobata Roxb. According to this screening, the direction of transmission of additional proteins depended upon the combination tested. While some graft partners failed to show exchange, some behaved as “donor” for additional proteins and still others could be both “donor” or “acceptor”. However, whether used as scion or stock, C. sativus was consistently identified as an acceptor. The occurrence of additional proteins in heterografts is discussed with regard to the transport mechanism of structural P-proteins in the phloem and its relationship to assimilate transport.
    Planta 09/1998; 206(4):630-640. · 3.38 Impact Factor
  • Bettina. Golecki
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    ABSTRACT: Kiel, Universiẗat, Diss., 1998 (Nicht für den Austausch).

Publication Stats

203 Citations
20.21 Total Impact Points

Institutions

  • 2013
    • Landeslabor Schleswig-Holstein
      Neumünster, Schleswig-Holstein, Germany
  • 1998–2006
    • Christian-Albrechts-Universität zu Kiel
      • • Institute of Phytopathology
      • • Botanisches Institut und Botanischer Garten
      Kiel, Schleswig-Holstein, Germany