[Show abstract][Hide abstract] ABSTRACT: Ischemia and reperfusion (I/R) leads to oxidative stress with free radical formation. With respect to liver surgery and transplantation this can lead to deleterious clinical effects. Protection of the liver against I/R injury is of major concern. Thus, in this study, we examined the effect of an antioxidant vitamin solution (vitamin E, C and ß-carotene) on warm I/R injury. Twelve pigs of the German landrace (7 animals in the vitamin group and 5 untreated controls) were examined in this animal model. Twenty-four hours before laparotomy, the vitamin group was initiated with a single intravenous infusion of the vitamin cocktail. The duration of complete warm ischemia of the liver was 4 hours. Serum liver enzyme levels (AST and ALT) and with thiobarbituric acid reacting substances (TBARS) in liver tissue were measured. Furthermore, immunohistochemical staining of oxidative products (oxidized proteins and 4-hydroxy-nonenal = 4-HNE) in liver tissue was made. The maximum accumulation of oxidized proteins was seen six days postoperatively in the controls whereas in the vitamin group only small amounts were seen. 4-HNE showed a marked accumulation in the controls but was almost not detectable in the vitamin group. TBARS were lower in the vitamin group compared to controls. Although the emulsifier necessary for the vitamin solution leads to increased liver enzyme levels in the vitamin group, the values returned to normal more rapidly. Antioxidant vitamins are able to improve warm I/R liver injury. Oxidative stress is directly verifiable at the tissue level. Future animal experiments as well as clinical trials are necessary to explore the optimization of the combination of antioxidative vitamins for the maximum protection from I/R injury. Key words: Ischemia and reperfusion -liver – oxidative stress – antioxidative vitamins.
[Show abstract][Hide abstract] ABSTRACT: A unique feature of human soft tissue liposarcoma is a stable (12;16)(q13;p11) translocation observed mainly in myxoid and roundcell liposarcomas. This translocation results in FUS/CHOP fusion transcripts with a corresponding oncogenic protein. We hypothesised that genes downstream of FUS/CHOP might serve as attractive candidates for novel tumour associated antigens. Among a panel of analysed genes, only pentraxin related gene (PTX3) demonstrated high expression in liposarcomas as compared to normal tissues. The analysis of RNA and protein expression demonstrated concordant results. However, the level of RNA and protein overexpression did not correlate in all cases. Finally, PTX3 expression was not related to presence of a FUS/CHOP fusion transcript within the liposarcoma tissues. PTX3 has been associated with adipocyte differentiation and now, additionally, is characterised by a markedly increased expression in human soft tissue liposarcoma. This finding mandates further research efforts to clarify the exact role of PTX3 in liposarcoma oncogenesis.
European Journal of Cancer 11/2006; 42(15):2639-46. DOI:10.1016/j.ejca.2006.05.035 · 5.42 Impact Factor