[Show abstract][Hide abstract] ABSTRACT: Introduction: Viral protein R (Vpr) of human immunodeficiency virus type 1 (HIV-1) has been
described as being involved in the progression of AIDS, and specific mutations are associated
with long-term non-progressor patients.
Case presentation: We describe the case of a child with repeated ear infections who was
otherwise healthy. The patient, a 5-year-old boy, was HIV-1 positive and the viral load at
admission was 1 073 899 RNA copies ml21 and 0 % CD4+ lymphocytes. A detailed study of the
vpr gene sequence of the child revealed mutations leading to amino acid substitutions at
positions 3 and 77.
Conclusion: The case reported provides clinical support of previous findings that show that the
R77Q and Q3R HIV-1 Vpr variants are associated with patients with delayed disease
[Show abstract][Hide abstract] ABSTRACT: Abstract We conducted a four-year (2003-2006) retrospective study of yeasts recovered in a hospital laboratory in the centre of Portugal to evaluate the epidemiology of yeast infections. Clinical isolates and data were gathered from 751 patients corresponding to 906 episodes of yeast infection. The isolates were first identified using classical and commercial methods, routinely employed at the hospital laboratory. We then re-identified the same isolates using RFLP of the ITS 5.8S rRNA gene and sequence of the D1/D2 domain of the 26S rRNA gene. Candida parapsilosis isolates were re-identified using the Ban I digestion of the SADH gene. C. albicans was the most frequently isolated of the yeasts found in the analysed specimens, with an overall incidence of 69.6% and then in decreasing order, C. glabrata, C. tropicalis, C. parapsilosis and C. krusei. C. parapsilosis was most frequently recovered from younger patients, decreasing with age, while C. glabrata occurrence increased with age. We found an increased number of cases of fungemia per 100,000 people per year, reaching a maximum of 4.4 during 2006.
Medical mycology: official publication of the International Society for Human and Animal Mycology 03/2009; 47(8):836-44. DOI:10.3109/13693780802709081 · 2.26 Impact Factor