Adolfo Cárdenas

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Publications (2)0 Total impact

  • Article: panD, a new chromosomal locus of Salmonella typhimurium for the biosynthesis of β-alanine
    Manuel V. Ortega, Adolfo Cárdenas, Dolores Ubiera
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    ABSTRACT: Three mutants of Salmonella typhimurium LT2, which required either pantothenate or -alanine for growth, were obtained after treatment with N-methyl-N-nitro-N-nitrosoguanidine. Their phenotype was: SM30 Pan-, SM31 Pan- Met-, SM32 Pan- Thi- (requirement for the thiazole-moiety of thiamine). Neither aspartate, dihydrouracil, nor -ureidopropionate replaced -alanine as growth factor. By conjugation it was found that the three genetic lesions (Pan-, Met-, Thi-) were located at about minute 128 of the bacterial chromosome. By transduction 63% linkage was found between the Pan and Met loci, and 84% between the Thi and Pan loci. Probably the thiazole auxotrophy was due to a lesion in the thiG locus. The new genetic locus responsible for the synthesis of -alanine was named panD.
    MGG - Molecular and General Genetics 01/1975; 140(2):159-164.
  • Article: [Larrea tridentata. Effect on a non-metabolic model of urolithiasis in rats].
    Eliseo Portilla-de Buen, Luisa Ramos, Abigail Aguilar, Alberto Ramos, David García-Martínez, Adolfo Cárdenas, Sergio Rodríguez-Reynoso, Caridad Leal
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    ABSTRACT: To evaluate the effect of L. tridentata infusion on the development of urinary calculi in a non-metabolic model. Male Wistar rats were divided into two groups (n = 10 each). The experimental group received 1 mL of oral L. tridentata infusion three times daily for 95 days. Control group received 1 mL tap water. Five days after initiating treatment, urolithiasis was induced inserting a series of 15 knots of 5-0 chromic catgut into the urinary bladder. Measurements included body weight and water intake, complete blood counts, glucose, BUN, creatinine, bacterial culture, and weight of urinary stones and sands. No statistically significant differences were found between groups for any variable. An infusion of L. tridentata was not effective in the prevention of urolith formation in the suture-induced rat model. It produced no alterations in body weight gain, blood counts, or water intake. Future work is needed to completely rule out any effect of the plant on urolith formation.
    Revista medica del Instituto Mexicano del Seguro Social 46(5):519-22.
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