Publications (2)0 Total impact
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ABSTRACT: Bone grafting procedures are widely used to repair large defects successfully in humans, but new surgical therapies can be designed to improve allograft integration. The objective of this study was to investigate the best surgical procedure to study bone graft integration in a large animal model. An osteoperiosteal defect of 3 cm in the tibia or in the metatarsal was made in 15 adult crossbreed sheep to investigate osteo-integration of a homologous bone graft in an intercalary critical defect. DCP plates, alone or in association with Scotchcast or external fixator were used as fixation devices. The Scotchcast as was applied after surgery and left for 2 months to avoid torsion stress of the limb during the stand up movement. Metatarsal defect fixed with 7-hole DCP plate and protected with Scotchcast was the best surgical approach to avoid early or late implant failures, and provided good radiographic results after 4 months.La Chirurgia degli Organi di Movimento 12/2005; 90(1):31-9.
Conference Proceeding: Growth factors contained in platelets enhance proliferation of human mesenchymal stem cells[show abstract] [hide abstract]
ABSTRACT: Mesenchymal stem cells (MSC) contained in the bone marrow provide an invaluable source for for bone reconstruction. Major limitations in their use are: the relative low number of MSC in the marrow cell population and the small volume of bone marrow available. There are emerging data demonstrating clinical applications of ex-vivo expanded cells, whose expansion is supported by several growth factors. We investigated whether growth factors (GFs) released by platelets stimulated MSC proliferation. MTT assays showed that the effect of GFs on MSC proliferation was dose dependent and was maximal at 10%. Also frozen GFs were able to stimulate proliferation. In conclusion we identified a method to promote ex-vivo expansion of MSC to be used in bone reconstruction.Molecular, Cellular and Tissue Engineering, 2002. Proceedings of the IEEE-EMBS Special Topic Conference on; 02/2002