A X Cao

Publications (3)6.29 Total impact

• Article: Acrodictys (Hyphomycetes) and related genera from China

  G. Z. Zhao, A. X. Cao, T. Y. Zhang, X. Z. Liu
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  ABSTRACT: Taxonomic studies on Acrodictys and related genera in China has yielded 13 taxa including a new species, Acrodictys porosiseptata sp. nov., characterized by indeterminate conidiophores with successive lageniform proliferations and muriform conidia usually with 4–5 parallel transverse septa, several longitudinal or oblique septa, and the presence of conspicuous septal pores. Acrodictys atroapicula, A. irregularis, A. micheliae, Pseudoacrodictys deightonii, and Rhexoacrodictys fuliginosa are newly recorded for China. Descriptions and illustrations of all taxa are provided, and similarities or differences with morphologically close taxa are discussed. KeywordsAnamorphic ascomycota–Dictyosporous fungi–Taxonomy
  Mycological Progress 04/2012; 10(1):67-83. · 1.55 Impact Factor
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  Available from: Cheng Yi

  Article: Identification of WA-type three-line hybrid rice with real-time polymerase chain reaction (PCR) method.

  Y Cheng, B D Gao, H Y Chen, J J Mao, A X Cao, J G Zhu, S F Zhu
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  ABSTRACT: A real-time fluorescent PCR (RTF-PCR) was developed to detect and quantify wild abortive (WA)-type three-line hybrid rice (Oryza sativa L.). The mitochondrial R₂₋₆₃₀ WA gene was reported to be closely related to male sterility in plants, and developed as a molecular maker to identify the cytoplasmic male sterility system of hybrid rice. First, we got the DNA sequence of R₂₋₆₃₀ WA gene in 17 rice species with traditional PCR. Then, a pair of specific primers (P₃, P₄) and TaqMan fluorescence probe (P₃₋₁₄) were designed based on the R₂₋₆₃₀ DNA sequence. The following RTF-PCR was performed on the 17 rice species finally. The results indicate that the probes used here are specific for three-line hybrid rice F₁ and male sterile lines. We can even identify a single hybrid seed using the probes, which confirmed that the probes can be applied to the identification and quantification of the WA-type three-line hybrid rice. In addition, the RFT-PCR system can be optimized when the annealing temperature is 60 °C and the Mg²⁺ concentration is 3.5 mmol/L.
  Applied biochemistry and biotechnology 12/2011; 166(4):819-29. · 1.94 Impact Factor
• Article: Detection of the Pinewood Nematode, Bursaphelenchus xylophilus, Using a Real-Time Polymerase Chain Reaction Assay.

  A X Cao, X Z Liu, S F Zhu, B S Lu
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  ABSTRACT: ABSTRACT The pinewood nematode, Bursaphelenchus xylophilus, has caused significant damage to pine plantations both in East Asia and North America and is an important quarantine organism. A real-time polymerase chain reaction (PCR) assay was developed to detect B. xylophilus. A set of primers and probe specific for B. xylophilus was designed to target the ribosomal DNA internal transcribed spacer region. Optimal primer concentration, Mg(2+) concentration, and extension temperature were 400 nM, 3.0 mM, and 60 degrees C, respectively. The assay was highly specific and sensitive, detecting as little as 0.01 ng of B. xylophilus DNA. The real-time PCR assay also successfully detected B. xylophilus in field samples, and it should be very useful for quarantine purposes.
  Phytopathology 06/2005; 95(5):566-71. · 2.80 Impact Factor