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Publications (4)1.73 Total impact

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    ABSTRACT: A study was undertaken to identify Marek’s disease virus (MDV) antigen by PCR and AGID and to test the significance of PCR and AGID by McNemar’s test in detection of MDV antigen in outbreak in layer flocks. A total of twelve different MD outbreak flocks with varying flock size were selected in this study. Feather follicles were collected from 10 apparently healthy birds, 10 clinically affected birds and 10 dead birds separately in each outbreak. All the samples were subjected to PCR and AGID. In PCR, 42 (35.00%), 68 (56.67%) and 106 (88.33%) samples were positive to MDV in apparently healthy birds, clinically affected birds and dead birds respectively and in AGID 28 (23.33%), 56 (46.67%) and 98 (81.67%) samples were positive to MDV in apparently healthy birds, clinically affected birds and dead birds respectively. In testing the significance of PCR and AGID in detecting MDV, significant difference existed between the two tests in feather tips of apparently healthy birds (P < 0.05), whereas there was no significant difference between PCR and AGID in detection of MDV in feather tips of clinically affected and dead birds (P > 0.05). Hence, PCR can be used to screen MDV in apparently healthy birds and AGID can be used to screen MDV in clinically affected and dead birds keeping feasibility and economic consideration. [Vet. World 2010; 3(5.000): 212-214]
    Veterinary World. 01/2010;
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    gkmurthy_in@yahoo.com, Natarajan Dorairajan, Gurusamypalayam Amirthalingam Balasubramanium, Arunachalapillai Manicavasaka Dinakaran, Kulandaivelu Saravanabava
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    ABSTRACT: Eighteen isolates of Ornithobacterium rhinotracheale isolated from laying hens in India were tested for their susceptibility to various antibiotic agents. Antibiogram pattern of all the 18 O. rhinotracheale isolates were determined in Mueller Hinton agar enriched with 10 per cent sheep blood, with antibiotic discs. All the isolates were resistant to amikacin, cloxacillin, co-trimoxazole, gentamicin, metronidazole and triple sulpha. Susceptibility of O. rhinotracheale isolates to cephalexin, norfloxacin, pefloxacin, streptomycin and furazolidone was variable. The isolates were sensitive to amoxycillin, ampicillin, chloramphenicol, ciprofloxacin, doxycycline, enrofloxacin, erythromycin, oxytetracycline, and penicillin-G.
    Veterinary Archives (veterinarski.arhiv@vef.hr); Vol.78 No.1. 01/2008;
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    ABSTRACT: The effect of vaccination of chickens with different inactivated vaccines against experimental Ornithobacterium rhinotracheale challenge was investigated. Eight different vaccines, with different inactivating substances (Formalin and thiomersal) and with or without adjuvant (mineral oil, alum and aluminium hydroxide gel), were produced. Following vaccination of experimental chickens at week 8 with formalin-inactivated mineral oil adjuvant bacterin, the mean O. rhinotracheale antibody titres rose to 5.88 2log 21 days after primary vaccination and enhanced to a titre of 6.59 2log 21 days after booster vaccination. The bacterin in mineral oil adjuvant induced the highest serologic response and a significant decrease of lesions such as air sacculitis and pneumonia in vaccinated birds compared with the unvaccinated challenge control birds. The bacterin in either alum or aluminium hydroxide gel adjuvant induced a moderate serologic response and a decrease of lesions compared with the unvaccinated challenge controls. The study showed that vaccination of layer chicken at the eighth week followed by a booster dose at the 12th week of age can effectively protect against O. rhinotracheale infections.
    Avian Pathology 01/2008; 36(6):481-5. · 1.73 Impact Factor
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    M. Geetha, S. Malmarugan, A. Manicavasaka Dinakaran, Vishal Kumar Sharma, Rohit Kumar