Hugo Enrique Orsini Beserra

Publications (2)0.96 Total impact

• Article: Experimental canine model for sentinel lymph node biopsy in the vulva using technetium and patent blue dye.

  José Ulcijara Aquino, Luiz Gonzaga Porto Pinheiro, Paulo Henrique Diógenes Vasques, João Ivo Xavier Rocha, Diego Alves Cruz, Hugo Enrique Orsini Beserra, Raissa Vasconcelos Cavalcante
  [show abstract] [hide abstract]
  ABSTRACT: This paper aims to study and define the experimental model of sentinel lymph node biopsy of the vulva in bitches. 0.2 ml of 99mTc phytate was injected intradermally, using a fine gauge insulin needle in the anterior commissure of the vulva. Thirty minutes after 99mTc injection, the inguinal mapping was performed using a gamma probe. After this, 0.5 ml of blue dye (bleu patenté V Guerbet 2.5%) was injected in the same place. After 15 minutes, a 3 cm long inguinal incision was made at point maximum uptake followed by careful dissection, guided by visualization of a bluish afferent lymphatic system that points to the sentinel lymph node (SLN). It was observed that 88% of SLN were identified. It wasn't found a significant difference among the presence or not of sentinel lymph node in the sides, which is an indication of a good consistency. It was observed a high (88%) and significant (χ2=12.89 and p=0.0003) intercession between both methods (blue dye and radiation). The experimental model adopted is feasible, becoming advantageous in applying the association of Patent blue and 99mTc.
  Acta cirurgica brasileira / Sociedade Brasileira para Desenvolvimento Pesquisa em Cirurgia 02/2012; 27(2):102-8. · 0.48 Impact Factor
• Article: Hemosiderin: a new marker for sentinel lymph node identification.

  Luiz Gonzaga Porto Pinheiro, Renato Santos de Oliveira Filho, Paulo Henrique Diógenes Vasques, Pedro Henrique de Oliveira Filgueira, Douglas Henning Pinheiro Aragão, Pedro Macedo Esmeraldo Barbosa, Hugo Enrique Orsini Beserra, Raissa Vasconcelos Cavalcante
  [show abstract] [hide abstract]
  ABSTRACT: To evaluate and present our initial results of a new marker (hemosiderin) for mammary sentinel lymph node identification in an experimental model. Skins mapped like a lymphatic duct draining to the axilla in patients submitted to breast biopsy, in our mastology service, stimulated us to try it in an animal model (female dogs). Our theory was that some blood derivate (hemosiderin) was captured by macrophages and accessed the lymphatic ducts in direction to the axilla. Six female dogs of no defined race were studied. We injected 0,2 ml of technetium on both superior mammary glands. After ten minutes, a 2,5 ml solution of hemolized blood (hemosiderin) from the own animal was injected in the subareolar lymphatic plexus on the left superior mammary gland and 2,5 ml of patent blue concomitantly and equally on the contralateral gland. Ten minutes after, incisions on both axillae were made to search, through the lymphatic mapping and a gamma probe, the sentinel lymph nodes. Seven brown sentinel lymph nodes were identified and also radiomarked on the left axilla. Six blue sentinel lymph nodes were identified and also radiomarked on the right axilla. Preliminary studies of a potential new dye for sentinel lymph node identification are presented. It may be the change of the current use of the blue dyes and their severe side-effects on patients submitted to sentinel lymph node biopsies.
  Acta cirurgica brasileira / Sociedade Brasileira para Desenvolvimento Pesquisa em Cirurgia 24(6):432-6. · 0.48 Impact Factor