M L Leal

Publications (3)5.91 Total impact

• Article: Characterization and immunogenicity of meningococcal group C conjugate vaccine prepared using hydrazide-activated tetanus toxoid.

  I A F B Silveira, R C Bastos, M S Neto, A P Laranjeira, E F Assis, S A R Fernandes, M L Leal, W C Silva, C-H Lee, C E Frasch, J M Peralta, E Jessouroun
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  ABSTRACT: The steps to produce, purify and control an immunogenic Brazilian conjugate vaccine against group C meningococcus (MenCPS-TT) using hydrazide-activated tetanus toxoid were developed. The conjugation methodology reduced the reaction time easily allowing scale-up. One freeze-dried pilot vaccine lot purified by tangential filtration, showed satisfactory quality control results including safety and stability. The pilot vaccine was immunogenic in mice in a dose-dependent fashion generating a 10-20-fold rise in IgG response in mice. The vaccine also induced high bactericidal titers. Vaccine concentrations of 1 and 0.1 microg showed higher avidity indices, suggesting induction of immunologic memory. These results support initiation of Phase I clinical studies with the MenCPS-TT conjugate vaccine.
  Vaccine 11/2007; 25(41):7261-70. · 3.77 Impact Factor
• Article: Characterization and immunogenicity of meningococcal group C conjugate vaccine prepared using hydrazide-activated tetanus toxoid

  I.A.F.B. Silveira, R.C. Bastos, M.S. Neto, A.P. Laranjeira, E.F. Assis, S.A.R. Fernandes, M.L. Leal, W.C. Silva, C.-H. Lee, C.E. Frasch, J.M. Peralta, E. Jessouroun
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  ABSTRACT: The steps to produce, purify and control an immunogenic Brazilian conjugate vaccine against group C meningococcus (MenCPS–TT) using hydrazide-activated tetanus toxoid were developed. The conjugation methodology reduced the reaction time easily allowing scale-up. One freeze-dried pilot vaccine lot purified by tangential filtration, showed satisfactory quality control results including safety and stability. The pilot vaccine was immunogenic in mice in a dose-dependent fashion generating a 10–20-fold rise in IgG response in mice. The vaccine also induced high bactericidal titers. Vaccine concentrations of 1 and 0.1 μg showed higher avidity indices, suggesting induction of immunologic memory. These results support initiation of Phase I clinical studies with the MenCPS–TT conjugate vaccine.
  Vaccine.
• Source

  Available from: scielo.br

  Article: Study on thermostabilizers for trivalent oral poliomyelitis vaccine.

  M L Leal, F J Lopes, M H Carvalho, H A Moura, S A Soares
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  ABSTRACT: Different formulations of Trivalent Oral Poliomyelitis Vaccine were tested, in order to obtain better thermostability, reduce corrosion of machinery and improve production costs. Magnesium chloride, sucrose, arginine and 199-Hank's medium were used in the formulations. The most appropriate formulation was a mixture of MgCl2 and arginine, which was highly thermostable, and had low production costs.
  Memórias do Instituto Oswaldo Cruz 85(3):329-38. · 2.15 Impact Factor