M Melzer

Publications (2)0 Total impact

• Article: Effects of surface proteins of human erythrocyte membrane on the interaction with lipopolysaccharides from Escherichia coli O55:B5

  D. S. Kabanov, A. Yu. Ivanov, M. Melzer, I. R. Prokhorenko
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  ABSTRACT: The role of erythrocytic surface membrane proteins and membrane charge in the interactions of the erythrocytes with lipopolysaccharides (LPS) isolated from Escherichia coli O55:B5 (LPS E. coli , S-form) has been examined by two independent methods, flow cytometry and cell electrophoresis. Treatment of erythrocytes with trypsin that modifies stereochemical properties of cell surface resulted in a 16% increase in the level of the erythrocyte fluorescence measured after their incubation with fluorescently labeled LPS E. coli . Electrophoretic mobility (EM) of the trypsin-treated erythrocytes was reduced by 16%. The removal of sialic acids from the erythrocyte surface with neuraminidase had no considerable effect either on the relative EM values or fluorescence intensity after the incubation of cells with LPS. The results suggest that the major role in the incorporation of the S-form LPS into the membrane of human erythrocytes is played by stereochemical factors, whereas the cell surface charge is less significant.
  Biochemistry (Moscow) Supplement Series A Membrane and Cell Biology 05/2008; 2(2):128-132.
• Article: [Effect of environmental factors on the composition of lipopolysaccharides released from the Rhodobacter capsulatus cell wall].

  S V Zubova, M Melzer, I R Prokhorenko
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  ABSTRACT: The goal of this study was to compare the environmental factors that determine viability of the gram-negative photosynthesizing bacteria Rhodobacter capsulatus (specifically, light and growth medium composition) and to assess the effect of these factors on the synthesis and composition of lipopolysaccharides released from the cell wall. Depletion of medium resulted in the release of lipopolysaccharides with a truncated polysaccharide fragment. It is concluded that, due to high viability of these bacteria, the main factor that induced lipopolysaccharide release from the cell wall, irrespective of culturing conditions, is cell division rather than cell death.
  Izvestiia Akademii nauk. Seriia biologicheskaia / Rossiiskaia akademiia nauk