J. Borrego

Instituto Madrileño de Investigación y Desarrollo Rural, Agrario y Alimentario, Madrid, Madrid, Spain

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Publications (34)31.96 Total impact

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    ABSTRACT: Background and Aims The identification of the genes involved in traits of interest is one of the main aims of current plant genetic studies. Although berry texture is a quality trait of great importance in table grapes, no quantitative trait loci or related gene has been described for this trait so far. Methods and Results In this work, the gene VvPel, which codes for a pectate lyase in the grapevine, was selected as a candidate gene for an association study. This gene was sequenced in a core collection of 96 table grape accessions, which was also characterised for several bunch and berry morphological traits, including diverse texture parameters measured by means of a texture meter. The population structure, the nucleotide and haplotype diversity, the protein structure, the existence of selection, and the linkage disequilibrium (LD) were evaluated. Thirty-two single nucleotide polymorphisms (SNPs) and 15 haplotypes were identified in the VvPel sequence, and LD was low enough to provide a high-resolution power. Some of the polymorphisms associated significantly with texture parameters and with bunch size, explaining part of the variation found for those traits. Conclusions Among the polymorphisms found in the gene VvPel, S1027 and S405/S441 in relation to berry texture, and S48/S1030 in relation to bunch size, are the most promising and would need to be investigated further as putative causal polymorphisms or markers for the respective traits. Significance of the Study The present work is the first genetic study on berry texture in the grapevine, and constitutes a starting point for a deeper understanding of the genetic basis of the natural variation for berry texture and bunch size.
    Australian Journal of Grape and Wine Research 01/2013; 19(2):193-207. · 2.96 Impact Factor
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    ABSTRACT: Distinctness, uniformity and stability (DUS) testing of varieties is usually required to apply for Plant Breeders' Rights. This exam is currently carried out using morphological traits, where the establishment of distinctness through a minimum distance is the key issue. In this study, the possibility of using microsatellite markers for establishing the minimum distance in a vegetatively propagated crop (grapevine) has been evaluated. A collection of 991 accessions have been studied with nine microsatellite markers and pair-wise compared, and the highest intra-variety distance and the lowest inter-variety distance determined. The collection included 489 different genotypes, and synonyms and sports. Average values for number of alleles per locus (19), Polymorphic Information Content (0.764) and heterozygosities observed (0.773) and expected (0.785) indicated the high level of polymorphism existing in grapevine. The maximum intra-variety variability found was one allele between two accessions of the same variety, of a total of 3,171 pair-wise comparisons. The minimum inter-variety variability found was two alleles between two pairs of varieties, of a total of 119,316 pair-wise comparisons. In base to these results, the minimum distance required to set distinctness in grapevine with the nine microsatellite markers used could be established in two alleles. General rules for the use of the system as a support for establishing distinctness in vegetatively propagated crops are discussed.
    Theoretical and Applied Genetics 09/2009; 119(7):1213-22. · 3.66 Impact Factor
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    ABSTRACT: "Malvasia" is a common name for different grape cultivars that have long been grown in Spain. In many cases, these cultivars are noted as being aromatic, sweet, and similar to Muscat in flavour. However, not all grapes that share this name exhibit these characteristics. This study compares the Malvasia cultivars in the Spanish Denominations of Origin with those grape cultivars grown in the grapevine collection of El Encin (Alcala de Henares, Spain) using morphological, isoenzymatic, and microsatellite analysis as well as a large bibliographic search of the studied cultivars. Despite their Malvasia denomination, some cultivars have been identified as synonyms of 'Macabeo', 'Alarije', 'Doha Blanca', 'Chasselas', or 'Planta Nova', all included on the official Spanish list of commercial grape cultivars. 'Malvasia de Sitges' and 'Malvasia de Lanzarote' have the characteristic flavour of Malvasia grapes and no synonyms were found among the cultivars grown in Spain, whereas 'Malvasia Rosada' resulted from a colour mutation in 'Malvasia de Sitges'.
    SPANISH JOURNAL OF AGRICULTURAL RESEARCH 01/2009; 7(3):563-571. · 0.66 Impact Factor
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    ABSTRACT: Malvasía es un nombre usado para designar diferentes variedades de vid que, desde antiguo, se cultivan en España. En muchos casos corresponde al patrón de variedad aromática, dulce y con sabor amoscatelado, pero en algunas ocasiones no supone más que un nombre que no asegura dichas características.
    ACE: Revista de enología, ISSN 1697-4123, Nº. 111, 2009. 01/2009;
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    ABSTRACT: A nuclear microsatellite analysis has been done using the collection of seedless and seeded table grape cultivars available at the Finca El Encín (IMIDRA). A total of 398 accessions have been genotyped using 25 microsatellite loci. The average of alleles per locus was of 10.64 and the PI was of 1.26 x 10-21. A comparison between genotypes, and morphology when needed, has allowed defining synonyms, homonyms, sports, and mistakes among the plant material. In this study, some light has been brought to groups of varieties like ‘Chasselas’, ‘Alphonse Lavallée’, and ‘Roseti’. Also a parentage analysis with table grape cultivars has been carried out using the unique genotype table, which included 309 accessions, accompanied by a thorough search in literature. In most cases, the information available about the crosses originating these cultivars was correct, particularly in seedless cultivars. Nevertheless, some cultivars like ‘Cardinal’ or ‘Ohanes’ could not arise from the crosses described or suggested in the literature, and alternative parents have been proposed. In other cases, where previous information could not be found or was incomplete, some light has been brought on the genetic origin of some cultivars; for instance, the ancestors for ‘Admirable de Courtiller’ or ‘Calmeria’ have been suggested. A mathematical analysis, in the form of likelihood ratios has been done to determine the reliability of the suggested crosses.
    01/2009: pages 115-122; ISHS.
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    American Journal of Enology and Viticulture 01/2009; 60(4):525-532. · 1.86 Impact Factor
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    ABSTRACT: Genetic relationships among important table-grape varieties were studied through nuclear and chloroplast microsatellite analysis. A total of 376 accessions were genotyped with 25 nuclear microsatellite loci using three multiplex PCRs. The average alleles per locus was 9.96, while the probability of identity was 1.66 x 10-21. A comparison among genotypes, morphology when needed, and literature data has defined synonyms, homonyms, sports, and mistakes among the plant material. In this study, groups of varieties such as Afus Ali, Ahmeur bou Ahmeur, Chasselas, and Muscat of Alexandria were clarified. A parentage analysis of table-grape cultivars was also carried out using a nonredundant genotype table, which included 273 accessions, accompanied by a thorough search in the literature and chloroplast haplotypes to define the direction of the crosses found. In most cases, the information available about the crosses originating these cultivars was correct, particularly in seedless cultivars. Nevertheless, some cultivars such as Cardinal could not originate from the crosses described or suggested in the literature and alternative parents have been proposed. In other cases, where previous information could not be found or was incomplete, some light has been shed on the genetic origin of some cultivars; for instance, the ancestors of Alphonse Lavallée, Admirable de Courtiller, and Calmeria have been suggested. A mathematical analysis, in the form of likelihood ratios, was done to determine the reliability of the suggested crosses.
    American Journal of Enology and Viticulture 01/2009; 60(1):35-42. · 1.86 Impact Factor
  • VinoTeq. 01/2008; 39:32-36.
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    ABSTRACT: The cultivated variety Pedro Ximenes is a seeded winegrape and Corinto bianco is a parthenocarpic seedless variety maintained in collection. To date, the two varieties have not been considered as related. In this study, identical DNA profiles were observed in both varieties for 25 microsatellite loci. Among morphological characteristics, they only differed in the presence or absence of seeds and in seed-related characteristics such as berry size, cluster density, and cluster size. These results suggest that both varieties arose from the same original embryo and one is a mutation of the other. Parentage analysis revealed the cultivar Gibi as a probable parent of Pedro Ximenes.
    American Journal of Enology and Viticulture 01/2007; 58(4):540-543. · 1.86 Impact Factor
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    ABSTRACT: More than 6,000 plants have been analyzed to evaluate a system based on 9 microsatellites that could be useful for most of the issues related to the genotyping of grapevine varieties: variety identification, DUS testing, and identification of Essentially Derived Varieties (EDVs). The 9 microsatellite markers selected are: VVS2, VVMDS, VVMD27, VVMD28, ssrVrZAG29, ssrVrZAG62, ssrVrZAG67, ssrVrZAG83 and ssrVrZAG112. The selection of these markers was based on different criteria: availability (public), map position (genetically independent), polymorphism (high), allele size range (that allows multiplexing), and 'quality' (ease of amplification, absence of known null alleles, absence of alleles differing only in 1 bp). A genotyping system was optimized, including the design of a multiplex PCR with the 9 markers, and capillary electrophoresis and fluorescence analysis in an automatic sequencer. More than 1,300 accessions (2,600 plants) of Vitis vinifera L. from the collection of grapevine varieties at the "EI Endn" state have been analyzed with this system. Until now, the conclusion reached for all those accessions studied that presented the same genotype at the 9 microsatellite Ioci is that they arise from the same embryo. Once the genotype table has been built up, any plant belonging to any of these varieties can be easily and unequivocally identified, with the exception of the sports and EDVs, which require a morphological description. Regarding the technical exams for legal protection of new varieties (DUS test), the lower number of different alleles between different varieties, and the higher number of different alleles within a variety (mutations) have been used to determine a minimum distance that allows the establishing of Distinction. Uniformity and Stability have been evaluated by studying about 4,000 plants of 19 different varieties.
    Bulletin de l'OIV. 01/2007; 80(914-915-916):177-185.
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    ABSTRACT: Grapevine rootstocks are a complex group of plants, most of them hybrids derived from native North American Vitis species that are used to provide resistance against phylloxera and soilborne problems. A representative group of rootstock accessions and cultivars of the Vitis species commonly used in rootstock breeding (V. vinifera, V. berlandieri, V. riparia, and V. rupestris) and conserved in the largest European germplasm banks of Vitis were analyzed using sequence tagged microsatellite sites (STMS) and amplified fragment length polymorphism (AFLP) markers. The STMS analysis allowed assigning a microsatellite genotype to most of rootstock cultivars, although it revealed numerous misclassified accessions in the studied collections. Genetic similarity among the different genotypes was analyzed using AFLP, which provided information on the genetic relationships within and between hybrid groups.
    American Journal of Enology and Viticulture 01/2007; 58(1):75-86. · 1.86 Impact Factor
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    BMT 10 SessionBMT 10 Session; 01/2006
  • Rivista di Viticoltura e di Enologia. 01/2006; 59(2-3):57-62.
  • Viticultura y Enología Profesional. 01/2006; 102:43-52.
  • 01/2006: pages 224; Instituto Canario de Calidad Agroalimentaria., ISBN: 8460639770
  • Tecnología del vino. 01/2005; 28:43-53.
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    ABSTRACT: In order to investigate the comparability of microsatellite profiles obtained in different laboratories, ten partners in seven countries analyzed 46 grape cultivars at six loci (VVMD5, VVMD7, VVMD27, VVS2, VrZAG62, and VrZAG79). No effort was made to standardize equipment or protocols. Although some partners obtained very similar results, in other cases different absolute allele sizes and, sometimes, different relative allele sizes were obtained. A strategy for data comparison by means of reference to the alleles detected in well-known cultivars was proposed. For each marker, each allele was designated by a code based on the name of the reference cultivar carrying that allele. Thirty-three cultivars, representing from 13 to 23 alleles per marker, were chosen as references. After the raw data obtained by the different partners were coded, more than 97% of the data were in agreement. Minor discrepancies were attributed to errors, suboptimal amplification and visualization, and misscoring of heterozygous versus homozygous allele pairs. We have shown that coded microsatellite data produced in different laboratories with different protocols and conditions can be compared, and that it is suitable for the identification and SSR allele characterization of cultivars. It is proposed that the six markers employed here, already widely used, be adopted as a minimal standard marker set for future grapevine cultivar analyses, and that additional cultivars be characterized by means of the coded reference alleles presented here. The complete database is available at http://www.genres.de/eccdb/vitis/ Cuttings of the 33 reference cultivars are available on request from the Institut National de la Recherche Agronomique Vassal collection (didier.vares@ensam.inra.fr).
    Theoretical and Applied Genetics 12/2004; 109(7):1448-58. · 3.66 Impact Factor
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    ABSTRACT: A total of 621 accessions of Vitis vinifera L. from the Spanish gene bank at Alcal de Henares (Madrid) were characterized using morphological descriptors, isoenzymes and microsatellites. Ampelographic evaluations included 27 morphometric plus 50 morphological characters. Of these twelve measurements or ratios were considered the most discriminant and 32 OIV descriptors were the most adequate for characterization because of their stability and objectiveness. Four isoenzyme systems provided 80 different patterns in these accessions. Analysis of six microsatellites in the studied samples resulted in 163 genotypes. The same microsatellite genotype was obtained for closely related varieties as in the case of mutations of the berry color, like Garnacha Blanca, Garnacha Gris and Garnacha Negra. Application of these characterization methods resulted in 177 accessions, which should be kept as a base collection, that represents less than 30% of the initial number.
    Genetic Resources and Crop Evolution 05/2004; 51(4):403-409. · 1.59 Impact Factor
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    ABSTRACT: A broad germplasm bank collection containing most of the autochthonous Spanish grapevine cultivars was analyzed using six sequence-tagged microsatellite site (STMS) loci: VVS2, VVMD5, VVMD7, ssrVrZAG47, ssrVrZAG62, and ssrVrZAG79. The number of alleles obtained ranged from 9 in ssrVrZAG47 to 13 in VVS2, and the observed genotypes per locus varied between 24 (ssrVrZAG47) and 41 (VVSS2). A total of 57 unique genotypes were obtained considering all 6 loci, and 40 varieties presented at least 1 of these specific genotypes. The genotypic combinations for the 6 loci have generated 163 different profiles in the 176 cultivars. Ten pairs of accessions and one group of four Garnacha's cultivars can not be differentiated. The observed heterozygosity varied between 75.6 (VVMD7) and 90.9% (VVMD5), without significant differences from the expected values for any loci. The VVMD5 locus was the most informative, and also showed the highest discrimination power. The cumulative discrimination power for all six loci was practically 1; however, in fact, these STMS loci have differentiated only about 93% of the accessions, probably owing to high relatedness of the plant material. Usefulness of this STMS set for characterization of a Spanish grapevine collection is emphasized, as well as the elaboration of databases with these molecular markers.
    Genome 03/2003; 46(1):10-8. · 1.67 Impact Factor
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    ABSTRACT: One hundred eleven Spanish accessions of Vitis vinifera L. that had been previously differentiated by a morphological and isoenzymatic survey have been analyzed in order to resolve existing doubts and to build an easily accessible database for comparison with existing databases. Ninety-six different genotypes were observed through the analysis of 13 microsatellite loci (VVMD5, VVMD7, VVMD27, VVMD28, VVS2, VVS5, VVS29, ssrVrZAG 29, ssrVrZAG 62, ssrVrZAG 67, ssrVrZAG 83, ssrVrZAG 79, and ssrVrZAG 112). Some of the coincidences found correspond to sports. Some other cases may be viewed as authentic synonymies, while others are errors. The analysis of genetic similarity does not allow for the drawing of any general conclusions about the origin of Spanish variety groupings, although the high proportion of alleles shared among certain varieties may suggest a common origin.
    American Journal of Enology and Viticulture 01/2003; 54(1):22-30. · 1.86 Impact Factor