Suman Ramteke

Publications of Suman Ramteke

• Preparation and evaluation of tubular micelles of pluronic lecithin organogel for transdermal delivery of sumatriptan.

  Authors: Varsha Agrawal, Vandana Gupta, Suman Ramteke, Piyush Trivedi

  AAPS PharmSciTech. 12/2010; 11(4):1718-25.

  The present work focuses on the preparation and evaluation of lecithin organogel system of thermoreversible polymer pluronic F127, which would enhance the stability and absorption of sumatriptanThe present work focuses on the preparation and evaluation of lecithin organogel system of thermoreversible polymer pluronic F127, which would enhance the stability and absorption of sumatriptan succinate across the skin. Formulations were developed with and without co-surfactant (pluronic F127). The prepared organogels were evaluated for its appearance, organoleptic characteristics, and feel upon application, homogeneity, occlusivenes, washability, pH, viscosity, spreadability, gel transition temperature of formulations. The formulations were also evaluated for drug content, in vitro drug diffusion properties and skin irritation testing. In vivo evaluation of formulations was carried out by hot plate and writhing test method, and finally the optimized formulation was subjected to stability studies. The developed formulations were easily washable, smooth in feel, and showed no clogging which indicate superior texture of system. Formulation, containing pluronic showed greater spreadability and higher drug diffusion rate as compared to pluronic free organogel. Drug content of organogel formulations was in the range of 94-97%. The pH of the formulations was 6.48 ± 0.5 and 6.98 ± 0.1, reflecting no risk of skin irritation. Pluronic not only enhances the stability of organogel by increasing the viscosity (from 6,541 ± 234.76 to 7,826 ± 155.65 poise) but also increases the release of drug from 67.39 ± 1.53% to 74.21 ± 1.7%. The sumatriptan exhibits higher and long lasting antinociceptive effect as indicated by the persistent increase in reaction time in hot plate and inhibited abdominal contraction in acetic acid-induced writhing test (p < 0.05). The prepared optimized formulation was found to be stable without any significant changes at room temperature.

• Enhancement of bioavailability of cefpodoxime proxetil using different polymeric microparticles.

  Authors: Fahim Khan, Rajesh Katara, Suman Ramteke

  AAPS PharmSciTech. 09/2010; 11(3):1368-75.

  Poorly water-soluble drugs such as cefpodoxime proxetil (400 μg/ml) offer a challenging problem in drug formulation as poor solubility is generally associated with poor dissolution characteristicsPoorly water-soluble drugs such as cefpodoxime proxetil (400 μg/ml) offer a challenging problem in drug formulation as poor solubility is generally associated with poor dissolution characteristics and thus poor oral bioavailability. According to these characteristics, preparation of cefpodoxime proxetil microparticle has been achieved using high-speed homogenization. Polymers (methylcellulose, sodium alginate, and chitosan) were precipitated on the surface of cefpodoxime proxetil using sodium citrate and calcium chloride as salting-out agents. The pure drug and the prepared microparticles with different concentrations of polymer (0.05-1.0%) were characterized in terms of solubility, drug content, particle size, thermal behavior (differential scanning calorimeter), surface morphology (scanning electron microscopy), in vitro drug release, and stability studies. The in vivo performance was assessed by pharmacokinetic study. The dissolution studies demonstrate a marked increase in the dissolution rate in comparison with pure drug. The considerable improvement in the dissolution rate of cefpodoxime proxetil from optimized microparticle was attributed to the wetting effect of polymers, altered surface morphology, and micronization of drug particles. The optimized microparticles exhibited excellent stability on storage at accelerated condition. The in vivo studies revealed that the optimized formulations provided improved pharmacokinetic parameter in rats as compared with pure drug. The particle size of drug was drastically reduced during formulation process of microparticles.

• Preparation and Characterization of Ethosomes for Topical delivery of Aceclofenac.

  Authors: A K Barupal, Vandana Gupta, Suman Ramteke

  Indian journal of pharmaceutical sciences. 09/2010; 72(5):582-6.

  The aim of present study was to prepare and characterized ethosomes of aceclofenac which may deliver the drug to targeted site more efficiently than marketed gel preparation and also overcome theThe aim of present study was to prepare and characterized ethosomes of aceclofenac which may deliver the drug to targeted site more efficiently than marketed gel preparation and also overcome the problems related with oral administration of drug. The formulations were prepared with varying the quantity of ethanol 10-50% (v/v), lecithin 1-4% (w/v), propylene glycol 5-20% (v/v) and evaluated for their vesicle size, shape and surface morphology, entrapment efficiency and in vitro drug permeation study. Ethosomes of average size of 1.112 μm with a spherical shape bearing smooth surface were observed by transmission electron microscopy and surface electron microscopy. The maximum entrapment of ethosomes was 91.06±0.79%. Cumulative amount of drug permeated through the biological membrane was found to be in the range of 0.26±0.014 to 0.49±0.032 mg/cm(2). Stability profile of prepared system was assessed for 45 days and the results revealed that very less degradation of drug was observed during storage condition.

• A new approach for PEGylation of dendrimers.

  Authors: Hemant Khambete, Surya P Gautam, C Karthikeyan, Suman Ramteke, N S Hari Narayana Moorthy, Piyush Trivedi

  Bioorganic & medicinal chemistry letters. 07/2010; 20(14):4279-81.

  Dendrimers have emerged as one of the most interesting themes for researchers as a result of unique functional architecture and macromolecular characteristics. The reported methods of PEGylation areDendrimers have emerged as one of the most interesting themes for researchers as a result of unique functional architecture and macromolecular characteristics. The reported methods of PEGylation are very time consuming and required multisteps for synthesis. In present work we have synthesized PEGylated polyamidoamine (PAMAM) dendrimers using epichlorohydrin as a linker. The PEGylated dendrimers were evaluated for color reaction UV, IR and NMR studies and compared with standard data.

• Novel Vesicular Approach For Topical Delivery of Baclofen Via Niosomes

  Authors: Raj K. KESERVANI , Anil K. SHARMA, Suman RAMTEKE

  Latin American Journal of Pharmacy. 01/2010; 29:1364-1370.

• Amoxicillin, clarithromycin, and omeprazole based targeted nanoparticles for the treatment of H. pylori.

  Authors: Suman Ramteke, N Ganesh, S Bhattacharya, Narendra Jain

  Journal of drug targeting. 03/2009;

  The aim of the present study was to develop and characterize targeted sustained release nanoparticles of chitosan-glutamic acid conjugates containing triple therapy for Helicobacter pylori to improveThe aim of the present study was to develop and characterize targeted sustained release nanoparticles of chitosan-glutamic acid conjugates containing triple therapy for Helicobacter pylori to improve its therapeutic effect and reduce its dose-related side effect. The chitosan-glutamate nanoparticles were prepared by using the ionotropic gelation method. The particle sizes of systems ranged between 550 nm and 900 nm. Percent drug entrapment and release profiles of amoxicillin, clarithromycin, and omeprazole in simulated gastric fluid (pH 1.2) were determined using high-performance liquid chromatography. The maximum % drug entrapment and % yield of formulations were about 60-90% and 73-88%, respectively. The stability of the drugs was assessed in simulated gastric fluid (pH 1.2). In vitro antibacterial efficacy of optimized formulations containing monotherapy and triple therapy on isolated culture of H. pylori was assessed. In vivo clearance study and histopathological study were also carried out on Swiss albino mice to evaluate the efficacy of triple therapy containing targeted nanosystem for the treatment of H. pylori. The proposed delivery system holds promise at optimizing the treatment of H. pylori.

• Triple therapy-based targeted nanoparticles for the treatment of Helicobacter pylori.

  Authors: Suman Ramteke, N Ganesh, S Bhattacharya, Narendra K Jain

  Journal of drug targeting. 11/2008; 16(9):694-705.

  The main aim of drug targeting was to increase the selectivity and efficacy of drugs at the infected site and to reduce the toxicity of biotechnological entities on other parts of the body. In theThe main aim of drug targeting was to increase the selectivity and efficacy of drugs at the infected site and to reduce the toxicity of biotechnological entities on other parts of the body. In the present study, we selected the triple therapy (amoxicillin, clarithromycin and omeprazole) due to its synergic and additive effects, which could drastically reduce the effective dose required for the complete eradication of Helicobacter pylori and prevent the development of antibiotic resistance. The gliadin nanoparticles (GNP) vis-à-vis lectin-conjugated gliadin nanoparticles (LGNP) of triple therapy were prepared by a desolvation method and evaluated for percent entrapment efficiency, percent yield, in vitro drug release study and mucoadhesion properties of formulations. The maximum percent entrapment efficiency of formulations was in the range of 65-85%. The percent yield of formulation was about 84 +/- 2.68%. The maximum percent cumulative release of all the three drugs from formulations was in the range of 22-76%. The specificity of lectin-conjugated particles was assessed by an in vitro agglutination assay method. The developed formulation was also assessed for in vitro antibacterial study, in vivo clearance efficacy and histopathological evaluation of formulations. In the in vitro antibacterial study, the LGNP with triple therapy showed a 94.83% eradication rate that is 7.4% more than that of GNP with triple therapy (88.28%) and about 16% more than that of the plain triple therapy (P < 0.001). The targeted lectin-conjugated formulations exhibited a superior in vivo clearance efficacy when compared to the non-conjugated formulations and plain drugs.

• Formulation Development and in vitro Characterization of Proliposomes for Topical Delivery of Aceclofenac.

  Authors: Vandana Gupta, Ashok K Barupal, Suman Ramteke

  Indian journal of pharmaceutical sciences. 11/2008; 70(6):768-75.

  Non-steroidal antiinflammatory drugs are routinely prescribed for the patients with rheumatic disease and such patients are at increased risk of serious gastrointestinal complications, whenNon-steroidal antiinflammatory drugs are routinely prescribed for the patients with rheumatic disease and such patients are at increased risk of serious gastrointestinal complications, when non-steroidal antiinflammatory drugs administered by oral route. The aim of the present study was to develop and characterized a vesicular drug carrier system (proliposome) for topical delivery of aceclofenac to overcome the problems related with oral route. Aceclofenac proliposome were prepared by the film-deposition on carriers method and characterized for size and surface morphology, drug content in both proliposomes and liposomal system, percent yield, in vitro drug release studies and drug permeation studies. The prepared system was also characterized for drug-excipients interaction by Fourier transform infrared spectrophotometer and stability studies. The size and surface morphology were studied using optical microscopy, scanning electron microscopy and transmission electron microscopy. A spherical shape of reconstituted aceclofenac liposome with an average vesicular size of about 500 nm was observed in photomicrographs. The maximum entrapment efficiency of reconstituted liposomes was 80.31% whereas the drug content in proliposomes was found to be more than 90%. In vitro release of drug was significantly retarded indicating sustained release of aceclofenac from proliposomes. Stability study was performed at various temperatures indicating that aceclofenac proliposomes are stable at lower temperature.

• Clarithromycin- and omeprazole-containing gliadin nanoparticles for the treatment of Helicobacter pylori.

  Authors: Suman Ramteke, Narendra K Jain

  Journal of drug targeting. 02/2008; 16(1):65-72.

  The aim of the present work was to prepare and evaluate the oral mucoadhesive sustained release nanoparticles of clarithromycin and omeprazole in order to improve patient compliance by improving itsThe aim of the present work was to prepare and evaluate the oral mucoadhesive sustained release nanoparticles of clarithromycin and omeprazole in order to improve patient compliance by improving its therapeutic effect and reducing its dose-related side effects. The clarithromycin- and omeprazole-containing gliadin nanoparticles were prepared by the desolvation method using Pluronic F-68 as a stabilizing agent. The results showed that this method is reproducible, easy, and leads to the efficient entrapment of drug as well as formation of spherical particles ranging from 400 to 650 nm. The maximum percentage of drug entrapment for clarithromycin and omeprazole was 81.7 +/- 2.2 and 73.7 +/- 3.9%, respectively, whereas the percentage of yield of the system was 85.1 +/- 1.9%. The sustained release behavior of gliadin nanoparticles was evaluated in phosphate-buffered saline (pH 7.4) and simulated gastric fluid (pH 1.2), at 37 +/- 1 degrees C. Their mucoadhesive properties were determined by in vitro and in vivo methods. In vitro antibacterial activity of the formulations was performed on isolated culture of Helicobacter pylori, which showed greater eradication effect of dual therapy entrapped formulations when compared with single therapy containing formulations and plain drugs.

• Formulation development and in vitro characterization of proliposomes for topical Delivery of aceclofenac

  Authors: Vandana Gupta, Ashok Barupal, Suman Ramteke

  Indian Journal of Pharmaceutical Sciences. 01/2008;

  Non-steroidal antiinflammatory drugs are routinely prescribed for the patients with rheumatic disease and such patients are at increased risk of serious gastrointestinal complications, whenNon-steroidal antiinflammatory drugs are routinely prescribed for the patients with rheumatic disease and such patients are at increased risk of serious gastrointestinal complications, when non-steroidal antiinflammatory drugs administered by oral route. The aim of the present study was to develop and characterized a vesicular drug carrier system (proliposome) for topical delivery of aceclofenac to overcome the problems related with oral route. Aceclofenac proliposome were prepared by the film-deposition on carriers method and characterized for size and surface morphology, drug content in both proliposomes and liposomal system, percent yield, in vitro drug release studies and drug permeation studies. The prepared system was also characterized for drug-excipients interaction by Fourier transform infrared spectrophotometer and stability studies. The size and surface morphology were studied using optical microscopy, scanning electron microscopy and transmission electron microscopy. A spherical shape of reconstituted aceclofenac liposome with an average vesicular size of about 500 nm was observed in photomicrographs. The maximum entrapment efficiency of reconstituted liposomes was 80.31% whereas the drug content in proliposomes was found to be more than 90%. In vitro release of drug was significantly retarded indicating sustained release of aceclofenac from proliposomes. Stability study was performed at various temperatures indicating that aceclofenac proliposomes are stable at lower temperature.

• Clarithromycin based oral sustained release nanoparticulate drug delivery system

  Authors: Suman Ramteke, Uma Maheshwari R, N Jain

  Indian Journal of Pharmaceutical Sciences. 01/2006;

  The aim of the present work is to prepare and evaluate the oral mucoadhesive sustained release nanoparticles of clarithromycin in order to improve patient compliance by simplifing its administration,The aim of the present work is to prepare and evaluate the oral mucoadhesive sustained release nanoparticles of clarithromycin in order to improve patient compliance by simplifing its administration, improving its therapeutic effect and reducing its dose related side effect. Clarithromycin containing gliadin nanoparticles were prepared by desolvation method using pluronic F-68 ® as a stabilizing agent. The results showed that this method is reproducible, very easy and led to the efficient entrapment of drug as well as formation of spherical particles ranging from 250-500 nm. Some process variables like effect of gliadin concentration and effect of surfactant were also evaluated with respect to their % drug entrapment and % yields. The maximum % drug entrapment and % yield were about 73 and 88%, respectively. The sustained release behavior of gliadin nanoparticles were evaluated both in phosphate buffer saline (pH 7.4) and simulated gastric fluid (pH 1.2), respectively, at 37±1°. Their mucoadhesive properties were determined by in vitro and in vivo methods. The shelf life of prepared nanoparticles was determined by storage at various temperatures while assessed in simulated gastric fluid (pH 1.2) with and without enzyme.

• Anti-Helicobacter pylori effect of mucoadhesive nanoparticles bearing amoxicillin in experimental gerbils model.

  Authors: R B Umamaheshwari, Suman Ramteke, Narendra Kumar Jain

  AAPS PharmSciTech. 02/2004; 5(2):e32.

  The purpose of the present study was to design mucoadhesive gliadin nanoparticles (GNP) containing amoxicillin and to evaluate their effectiveness in eradicating Helicobacter pylori. GNP-bearingThe purpose of the present study was to design mucoadhesive gliadin nanoparticles (GNP) containing amoxicillin and to evaluate their effectiveness in eradicating Helicobacter pylori. GNP-bearing amoxicillin (AGNP) was prepared by desolvation method. The effect of process variables such as gliadin concentration and initial drug loading on particle size, shape, percent payload, percent entrapment efficiency, in vitro release profile, and mucoadhesive property of GNP was assessed. Rhodamine isothiocyanate-entrapped GNP formulations were prepared to evaluate their in vivo gastric mucoadhesive property in albino rats. With increasing gliadin concentration, the mucoadhesive property of GNP increased. Typically, the maximum amount of nanoparticles remaining was 82 +/- 4%, which represented a stronger mucoadhesive propensity and specificity of GNP toward the stomach. In vitro antimicrobial activity of AGNP was evaluated by growth inhibition studies on an isolated H pylori strain. The time required for complete eradication was higher in AGNP than in amoxicillin because of the controlled drug delivery of amoxicillin from AGNP. In vivo clearance of H pylori following oral administration of AGNP to infected Mongolian gerbils was examined. Amoxicillin and AGNP both showed anti-H pylori effects in this experimental model of infection, but the required dose for complete eradication was less in AGNP than in amoxicillin. In conclusion, AGNP eradicated H pylori from the gastrointestinal tract more effectively than amoxicillin because of the prolonged gastrointestinal residence time attributed to mucoadhesion. A dosage form containing mucoadhesive nanoparticles bearing a potential antibiotic should be useful for the complete eradication of H pylori.