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ABSTRACT: The gene encoding NADH dehydrogenase from an alkalophile, Bacillus sp., was cloned and sequenced. The cloned DNA fragment contained an open reading frame of 1,657 nucleotides which encodes a polypeptide composed of 519 amino acid residues ( M r 55,830). The predicted amino acid sequence was consistent with the partial amino acid sequences including the N-terminal and C-terminal sequences determined in a previous study. Sequence comparison with other navoenzymes revealed high homology between the present dehydrogenase and Escherichia coli thioredoxin reductase.